A multiplex degenerate PCR analytical approach targeting to eight genes for screening GMOs

Food Chemistry

Volumn 132, Issue 3, 2012, Pages 1566-1573

  Guo, Jinchao ^a   Chen, Lili ^b   Liu, Xin ^c   Gao, Ying ^a   Zhang, Dabing ^a   Yang, Litao ^a  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

^b SHANGHAI NORMAL UNIVERSITY   (China)

^c AGRO ENVIRONMENTAL PROTECTION INSTITUTE   (China)

Author keywords

Genetically modified organisms; Quadruplex degenerate PCR; Trait gene

Indexed keywords

ACETYL TRANSFERASE; ANALYTICAL APPROACH; BACILLUS THURINGIENSIS; DETECTION METHODS; GENETICALLY MODIFIED FOOD; GENETICALLY MODIFIED ORGANISMS; HIGH SPECIFICITY; LIMIT OF DETECTION; LOWER COST; PCR ASSAY; PHOSPHINOTHRICIN; QUADRUPLEX DEGENERATE PCR; SCREENING APPROACHES; STREPTOMYCES HYGROSCOPICUS; TUMEFACIENS;

BACTERIA; BACTERIOLOGY; DNA SEQUENCES; DRIERS (MATERIALS); GENES; SCREENING;

POLYMERASE CHAIN REACTION;

ACYLTRANSFERASE; CP4 EPSPS PROTEIN; CRY1AB TOXIN; CRY1AC TOXIN; CRY3BB1 PROTEIN; MCRY3A PROTEIN; PHOSPHINOTHRICIN ACETYLTRANSFERASE; PROTEIN; UNCLASSIFIED DRUG;

ACETOBACTERIUM; ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL STRAIN; CONTROLLED STUDY; DNA SEQUENCE; GENE; LIMIT OF DETECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STREPTOMYCES; STREPTOMYCES HYGROSCOPICUS;

ACETOBACTERIUM; BACILLUS THURINGIENSIS; STREPTOMYCES HYGROSCOPICUS; STREPTOMYCES VIRIDOCHROMOGENES;

EID: 84856235106     PISSN: 03088146     EISSN: 18737072     Source Type: Journal    
DOI: 10.1016/j.foodchem.2011.11.096     Document Type: Article

References (29)

1. AGBIOS GM Crop Database. Available from: http://www.cera-gmc.org/?action= gm-crop-database [accessed 15.08.10].
2. K. Arumuganathan, and E.D. Earle Nuclear DNA content of some important plant species Plant Molecular Biology Reporter 9 1991 208 218
3. C. Bahrdt, A.B. Krech, A. Wurz, and D. Wulff Validation of a newly developed hexaplex real-time PCR assay for screening for presence of GMOs in food, feed and seed Analytical and Bioanalytical Chemistry 396 2010 2103 2112
4. F. Dahl, M. Gullberg, J. Stenberg, U. Landegren, and M. Nilsson Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments Nucleic Acids Research 33 2005 e71
5. M. Digiaro, T. Elbeaino, and G.P. Martelli Development of degenerate and species-specific primers for the differential and simultaneous RT-PCR detection of grapevine-infecting nepoviruses of subgroups A, B and C Journal of Virological Methods 141 2007 34 40
6. W. Dong, L. Yang, K. Shen, B. Kim, G.A. Kleter, H.J. Marvin, R. Guo, W. Liang, and D. Zhang GMDD: A database of GMO detection methods BMC Bioinformatics 9 2008 260
7. H.H. Dörries, I. Remus, A. Grönewald, C. Grönewald, and K. Berghof-Jäger Development of a qualitative, multiplex real-time PCR kit for screening of genetically modified organisms (GMOs) Analytical and Bioanalytical Chemistry 396 2010 2043 2054
8. EC decision (2005/317/EC) on Bt10 (2005). Available from: http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2005:101:0014:0016: EN:PDF.
9. EC decision (2006/601/EC) on LL601 (2006). Available from: http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2006:244:0027:0029: EN:PDF.
10. GMO Detection method Database (GMDD). Available from: http://gmdd.shgmo.org/. [accessed 10.08.10].
11. L. Grohmann, C. Brünen-Nieweler, A. Nemeth, and H.U. Waiblinger Collaborative trial validation studies of real-time PCR-based GMO screening methods for detection of the bar gene and the ctp2-cp4epsps construct Journal of Agricultural and Food Chemistry 57 2009 8913 8920
12. J. Guo, L. Yang, X. Liu, X. Guan, L. Jiang, and D. Zhang Characterization of the exogenous insert and development of event-specific PCR detection methods for genetically modified Huanong No. 1 papaya Journal of Agricultural and Food Chemistry 57 2009 7205 7212
13. A. Holst-Jensen Testing for genetically modified organisms (GMOs): Past, present and future perspectives Biotechnology Advances 27 2009 1071 1082
14. A. Holst-Jensen, S. Ronning, A. Lovseth, and K. Berdal PCR technology for screening and quantification of genetically modified organisms Analytical and Bioanalytical Chemistry 375 2003 985 993
15. C. James Global status of commercialized biotech/GM crops: 2009 ISAAA Briefs 2009 2010 41
16. J. Liu, J. Guo, H. Zhang, N. Li, L. Yang, and D. Zhang Development and in-house validation of the event-specific polymerase chain reaction detection methods for genetically modified soybean MON89788 based on the cloned integration flanking sequence Journal of Agricultural and Food Chemistry 57 2009 10524 10530
17. I.J. Lu, C.H. Lin, and T.M. Pan Establishment of a system based on universal multiplex-PCR for screening genetically modified crops Analytical and Bioanalytical Chemistry 396 2010 2055 2064
18. J. Mano, T. Oguchi, H. Akiyama, R. Teshima, A. Hino, S. Furui, and K. Kitta Simultaneous detection of recombinant DNA segments introduced into genetically modified crops with multiplex ligase chain reaction coupled with multiplex polymerase chain reaction Journal of Agricultural and Food Chemistry 57 2009 2640 2646
19. J. Mano, N. Shigemitsu, S. Futo, H. Akiyama, R. Teshima, A. Hino, S. Furui, and K. Kitta Real-time PCR array as a universal platform for the detection of genetically modified crops and its application in identifying unapproved genetically modified crops in Japan Journal of Agricultural and Food Chemistry 57 2009 26 37
20. D. Morisset, D. Dobnik, S. Hamels, J. Zel, and K. Gruden NAIMA: Target amplification strategy allowing quantitative on-chip detection of GMOs Nucleic Acids Research 36 2008 e118
21. A. Nadal, A. Coll, J.L. La Paz, T. Esteve, and M. Pla A new PCR-CGE (size and color) method for simultaneous detection of genetically modified maize events Electrophoresis 27 2006 3879 3888
22. H.S. Najafabadi, N. Torabi, and M. Chamankhah Designing multiple degenerate primers via consecutive pairwise alignments BMC Bioinformatics 9 2008 55
23. NCBI Entrez Nucleotide database. Available from: http://www.ncbi.nlm.nih. gov/nucleotide/ [accessed 15.05.10].
24. T.W. Prins, J.P. van Dijk, H.G. Beenen, A.A. Van Hoef, M.M. Voorhuijzen, C.D. Schoen, H.J. Aarts, and E.J. Kok Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction BMC Genomics 9 2008 584
25. M. Querci, M. Van den Bulcke, J. Zel, G. Van den Eede, and H. Broll New approaches in GMO detection Analytical and Bioanalytical Chemistry 396 2010 1991 2002
26. G.J. Randhawa, R. Chhabra, and M. Singh Multiplex PCR-based simultaneous amplification of selectable marker and reporter genes for the screening of genetically modified crops Journal of Agricultural and Food Chemistry 57 2009 5167 5172
27. T. Ruttink, R. Demeyer, E. Van Gulck, B. Van Droogenbroeck, M. Querci, I. Taverniers, and M. De Loose Molecular toolbox for the identification of unknown genetically modified organisms Analytical and Bioanalytical Chemistry 396 2010 2073 2089
28. H.K. Shrestha, K.K. Hwu, S.J. Wang, L.F. Liu, and M.C. Chang Simultaneous detection of eight genetically modified maize lines using a combination of event- and construct-specific multiplex-PCR technique Journal of Agricultural and Food Chemistry 56 2008 8962 8968
29. L. Yang, A. Pan, K. Zhang, C. Yin, B. Qian, J. Chen, C. Huang, and D. Zhang Qualitative and quantitative PCR methods for event-specific detection of genetically modified cotton Mon1445 and Mon531 Transgenic Research 14 2005 817 831