Assessments of Total and Viable Escherichia coli O157:H7 on Field and Laboratory Grown Lettuce

PLoS ONE

Volumn 8, Issue 7, 2013, Pages

  Moyne, Anne Laure ^a   Harris, Linda J ^a   Marco, Maria L ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DYE; PROPIDIUM MONOAZIDE; UNCLASSIFIED DRUG;

AGRICULTURAL INOCULATION; ARTICLE; BACTERIAL CELL; BACTERIAL COUNT; BACTERIAL STRAIN; BACTERIAL SURVIVAL; BACTERIUM COLONY; BACTERIUM DETECTION; CELL VIABILITY; CONTROLLED STUDY; ESCHERICHIA COLI; HUMIDITY; LETTUCE; LIMIT OF DETECTION; LIMIT OF QUANTITATION; NONHUMAN; PLANT GROWTH; PLANT PATHOGEN INTERACTION; REAL TIME POLYMERASE CHAIN REACTION;

BACTERIAL LOAD; ESCHERICHIA COLI O157; FOOD MICROBIOLOGY; LETTUCE; REAL-TIME POLYMERASE CHAIN REACTION; SHIGA TOXINS;

EID: 84880990838     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0070643     Document Type: Article

References (42)

1. Painter JA, Hoekstra RM, Ayers T, Tauxe RV, Braden CR, et al. (2013) Attribution of foodborne illnesses, hospitalizations, and deaths to food commodities by using outbreak data, United States, 1998-2008. Emerg Infect Dis 19: 407-415.
2. (CDC) Centers for Disease Control and Prevention (2006) Ongoing multistate outbreak of Escherichia coli serotype O157:H7 infections associated with consumption of fresh spinach-United States, September 2006. MMWR Morb Mortal Wkly Rep 55: 1045-1046.
3. (CDC) Centers for Disease Control and Prevention (2008) Outbreak of Salmonella serotype Saintpaul infections associated with multiple raw produce items-United States, 2008. MMWR Morb Mortal Wkly Rep 57: 929-934.
4. Jablasone J, Warriner K, Griffiths M, (2005) Interactions of Escherichia coli O157:H7, Salmonella typhimurium and Listeria monocytogenes plants cultivated in a gnotobiotic system. Int J Food Microbiol 99: 7-18.
5. Aruscavage D, Lee K, Miller S, LeJeune JT, (2006) Interactions affecting the proliferation and control of human pathogens on edible plants. J Food Sci 71: R89-R99.
6. Brandl MT, (2006) Fitness of human enteric pathogens on plants and implications for food safety. Annu Rev Phytopathol 44: 367-392.
7. Delaquis P, Bach S, Dinu LD, (2007) Behavior of Escherichia coli O157:H7 in leafy vegetables. J Food Prot 70: 1966-1974.
8. Franz E, van Bruggen AHC, (2008) Ecology of E. coli O157:H7 and Salmonella enterica in the primary vegetable production chain. Crit Rev Microbiol 34: 143-161.
9. Tyler HL, Triplett EW, (2008) Plants as a habitat for beneficial and/or human pathogenic bacteria. Annu Rev Phytopathol 46: 53-73.
10. Hirneisen KA, Sharma M, Kniel KE, (2012) Human enteric pathogen internalization by root uptake into food crops. Foodborne Pathog Dis 9: 396-405.
11. Brandl MT, Amundson R, (2008) Leaf age as a risk factor in contamination of lettuce with Escherichia coli O157:H7 and Salmonella enterica. Appl Environ Microbiol 74: 2298-2306.
12. Erickson MC, Webb CC, Diaz-Perez JC, Phatak SC, Silvoy JJ, et al. (2010) Surface and internalized Escherichia coli O157:H7 on field-grown spinach and lettuce treated with spray-contaminated irrigation water. J Food Prot 73: 1023-1029.
13. Bezanson G, Delaquis P, Bach S, McKellar R, Topp E, et al. (2012) Comparative Examination of Escherichia coli O157:H7 survival on romaine lettuce and in soil at two independent experimental sites. J Food Prot 75: 480-487.
14. Moyne AL, Sudarshana MR, Blessington T, Koike ST, Cahn MD, et al. (2011) Fate of Escherichia coli O157:H7 in field-inoculated lettuce. Food Microbiol 28: 1417-1425.
15. Islam M, Doyle MP, Phatak SC, Millner P, Jiang X, (2004) Persistence of enterohemorrhagic Escherichia coli O157:H7 in soil and on leaf lettuce and parsley grown in fields treated with contaminated manure composts or irrigation water. J Food Prot 67: 1365-1370.
16. Lindow SE, Brandl MT, (2003) Microbiology of the phyllosphere. Appl Environ Microbiol 69: 1875-1883.
17. Nocker A, Camper AK, (2009) Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques. FEMS Microbiol Lett 291: 137-142.
18. Kotewicz ML, Mammel MK, LeClerc JE, Cebula TA, et al. (2008) Optical mapping and 454 sequencing of Escherichia coli O157:H7 isolates linked to the US 2006 spinach-associated outbreak. Microbiology 154: 3518-3528.
19. Bettelheim KA, (1998) Reliability of CHROMagar O157 for the detection of enterohaemorrhagic Escherichia coli (EHEC) O157 but not EHEC belonging to other serogroups. J Appl Microbiol 85: 425-428.
20. Sharma VK, Bearson SMD, Bearson BL, (2010) Evaluation of the effects of sdiA, a luxR homologue, on adherence and motility of Escherichia coli O157:H7. Microbiology 156: 1303-1312.
21. Wang G, Clark CG, Rodgers FG, (2002) Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 Shiga toxin family by multiplex PCR. J Clin Microbiol 40: 3613-3619.
22. Chang B, Taguri T, Sugiyama K, Amemura-Maekawa J, Kura F, et al. (2010) Comparison of ethidium monoazide and propidium monoazide for the selective detection of viable Legionella cells. Jpn J Infec Dis 63: 119-123.
23. Varma M, Field R, Stinson M, Rukovets B, Wymer L, et al. (2009) Quantitative real-time PCR analysis of total and propidium monoazide-resistant fecal indicator bacteria in wastewater. Water Research 43: 4790-4801.
24. Van Frankenhuyzen JK, Trevors JT, Lee H, Flemming CA, Habash MB, (2011) Molecular pathogen detection in biosolids with a focus on quantitative PCR using propidium monoazide for viable cell enumeration. J Microbiol Meth 87: 263-272.
25. Dreux N, Albagnac C, Federighi M, Carlin F, Morris CE, et al. (2007) Viable but non-culturable Listeria monocytogenes on parsley leaves and absence of recovery to a culturable state. J Appl Microbiol 103: 1272-1281.
26. Liang N, Dong J, Luo L, Li Y, (2011) Detection of viable Salmonella in lettuce by propidium monoazide real-time PCR. J Food Sci 76: M234-M237.
27. Dinu LD, Bach S, (2011) Induction of viable but non-culturable Escherichia coli O157:H7 in the phyllosphere of lettuce: a food safety risk factor. Appl Environ Microbiol 77: 8295-8302.
28. Pan Y, Breidt F, (2007) Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells. Appl Environ Microbiol 73: 8028-8031.
29. Løvdal T, Hovda MB, Björkblom B, Møller SG, (2011) Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua. J Microbiol Meth 85: 164-169.
30. Contreras PJ, Urrutia H, Sossa K, Nocker A, (2011) Effect of PCR amplicon length on suppressing signals from membrane-compromised cells by propidium monoazide treatment. J Microbiol Meth 87: 89-95.
31. Nocker A, Cheung CY, Camper AK, (2006) Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J Microbiol Meth 67: 310-320.
32. Luo JF, Lin WT, Guo Y, (2010) Method to detect only viable cells in microbial ecology. Appl Microbiol Biotechnol 86: 377-384.
33. Sánchez G, Elizaquível P, Aznar R, (2012) A single method for recovery and concentration of enteric viruses and bacteria from fresh-cut vegetables. Int J Food Microbiol 152: 9-13.
34. Elizaquível P, Aznar R, (2008) A multiplex RTi-PCR reaction for simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Staphylococcus aureus on fresh, minimally processed vegetables. Food Microbiol 25: 705-713.
35. Maurer JJ (2006) The mythology of PCR: A warning to the wise. In: Maurer JJ, editor. PCR methods in foods. New York: Springer US. 27-40.
36. Brandl MT, (2008) Plant lesions promote the rapid multiplication of Escherichia coli O157:H7 on postharvest lettuce. Appl Environ Microbiol 74: 5285-5289.
37. Cooley MB, Chao D, Mandrell RE, (2006) Escherichia coli O157:H7 survival and growth on lettuce is altered by the presence of epiphytic bacteria. J Food Prot 69: 2329-2335.
38. Aurass P, Prager R, Flieger A, (2011) EHEC/EAEC O104:H4 strain linked with the 2011 German outbreak of haemolytic uremic syndrome enters into the viable but non-culturable state in response to various stresses and resuscitates upon stress relief. Environ Microbiol 13: 3139-3148.
39. Grey B, Steck TR, (2001) Concentrations of copper thought to be toxic to Escherichia coli can induce the viable but nonculturable condition. Appl Environ Microbiol 67: 5325-5327.
40. Méric G, Kemsley EK, Falush D, Saggers EJ, Lucchini S, (2013) Phylogenetic distribution of traits associated with plant colonization in Escherichia coli. Environ Microbiol 15: 487-501.
41. Gourabathini P, Brandl MT, Redding KS, Gunderson JH, Berk SG, (2008) Interactions between food-borne pathogens and protozoa isolated from lettuce and spinach. Appl Environ Microbiol 74: 2518-2525.
42. Quilliam RS, Williams AP, Jones DL, (2012) Lettuce cultivar mediates both phyllosphere and rhizosphere activity of Escherichia coli O157:H7. PLOS ONE 7: e33842.