A single method for recovery and concentration of enteric viruses and bacteria from fresh-cut vegetables

International Journal of Food Microbiology

Volumn 152, Issue 1-2, 2012, Pages 9-13

  Sanchez G ^a   Elizaquivel P ^b   Aznar, R ^a,b  

^a INSTITUTO DE AGROQUÍMICA Y TECNOLOGÍA DE ALIMENTOS CSIC   (Spain)

^b UNIVERSITY OF VALENCIA   (Spain)

Author keywords

Foodborne pathogens; Quantification; Real time PCR; Vegetables

Indexed keywords

MACROGOL; PEPTONE;

ARTICLE; BACTERIUM CONTAMINATION; COLONY FORMING UNIT; ESCHERICHIA COLI O157; FOOD CONTAMINATION; HEPATITIS A VIRUS; LIMIT OF DETECTION; LISTERIA MONOCYTOGENES; NONHUMAN; NOROVIRUS; POLYMERASE CHAIN REACTION; PRECIPITATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SALMONELLA ENTERICA; VEGETABLE; VIRAL CONTAMINATION; VIRUS DETECTION; ANIMAL; CLASSIFICATION; ENTEROBACTERIACEAE; ENTEROVIRUS; EVALUATION; FOOD CONTROL; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MOUSE; REAL TIME POLYMERASE CHAIN REACTION; VIROLOGY;

ESCHERICHIA COLI; HEPATITIS A VIRUS; LISTERIA MONOCYTOGENES; MURINE NOROVIRUS; NOROVIRUS; PETROSELINUM CRISPUM; SALMONELLA; SALMONELLA ENTERICA; SPINACIA OLERACEA;

ANIMALS; ENTEROBACTERIACEAE; ENTEROVIRUS; FOOD MICROBIOLOGY; HUMANS; LISTERIA MONOCYTOGENES; MICE; REAL-TIME POLYMERASE CHAIN REACTION; VEGETABLES;

EID: 84857130915     PISSN: 01681605     EISSN: 18793460     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2011.10.002     Document Type: Article

References (33)

1. Anonymous The community summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in the European Union in 2008. The EFSA Journal 2010, 1496.
2. Baert L., Uyttendaele M., Debevere J. Evaluation of viral extraction methods on a broad range of ready-to-eat foods with conventional and real-time RT-PCR for Norovirus GII detection. International Journal of Food Microbiology 2008, 123:101-108.
3. Berger C.N., Sodha S.V., Shaw R.K., Griffin P.M., Pink D., Hand P., Frankel G. Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environmental Microbiology 2010, 12:2385-2397.
4. Brassard J., Guevremont E., Gagne M.J., Lamoureux L. Simultaneous recovery of bacteria and viruses from contaminated water and spinach by a filtration method. International Journal of Food Microbiology 2011, 144:565-568.
5. Brehm-Stecher B., Young C., Jaykus L.A., Tortorello M.L. Sample preparation: the forgotten beginning. Journal of Food Protection 2009, 72:1774-1789.
6. Butot S., Putallaz T., Sanchez G. Procedure for rapid concentration and detection of enteric viruses from berries and vegetables. Applied and Environmental Microbiology 2007, 73:186-192.
7. Butot S., Putallaz T., Amoroso R., Sanchez G. Inactivation of enteric viruses in minimally processed berries and herbs. Applied and Environmental Microbiology 2009, 75:4155-4161.
8. Butot S., Le Guyader F.S., Krol J., Putallaz T., Amoroso R., Sanchez G. Evaluation of various real-time RT-PCR assays for the detection and quantitation of human norovirus. Journal of Virological Methods 2010, 167:90-94.
9. Cannon J.L., Papafragkou E., Park G.W., Osborne J., Jaykus L.A., Vinje J. Surrogates for the study of norovirus stability and inactivation in the environment: a comparison of murine norovirus and feline calicivirus. Journal of Food Protection 2006, 69:2761-2765.
10. Cheong S., Lee C., Choi W.C., Lee C.H., Kim S.J. Concentration method for the detection of enteric viruses from large volumes of foods. Journal of Food Protection 2009, 72:2001-2005.
11. Costafreda M.I., Bosch A., Pinto R.M. Development, evaluation and standardization of a real-time TaqMan reverse transcription-PCR assay for the quantification of hepatitis A virus in clinical and shellfish samples. Applied and Environmental Microbiology 2006, 72:3846-3855.
12. Croci L., Dubois E., Cook N., de Medici D., Schulz A.C., China B., Rutjes S.A., Hoofar J., van der Poel W.H. Current methods for extraction and concentration of enteric viruses from fresh fruit and vegetables: towards international standards. Food Analytical Methods 2008, 1:73-84.
13. Doyle M.P., Erickson M.C. Summer meeting 2007 - the problems with fresh produce: an overview. Journal of Applied Microbiology 2008, 105:317-330.
14. Elizaquível P., Aznar R. A multiplex RTi-PCR reaction for simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Staphylococcus aureus on fresh, minimally processed vegetables. Food Microbiology 2008, 25:705-713.
15. Elizaquível P., Gabaldón J.A., Aznar R. Quantification of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 in non-spiked food products and evaluation of real-time PCR as a diagnostic tool in routine food analysis. Food Control 2011, 22:158-164.
16. Ethelberg S., Lisby M., Bottiger B., Schultz A.C., Villif A., Jensen T., Olsen K.E., Scheutz F., Kjelso C., Muller L. Outbreaks of gastroenteritis linked to lettuce, Denmark, January 2010. Euro Surveillance 2010, 15.
17. Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couvé E., de Daruvar A., Dehoux P., Domann E., Domínguez-Bernal G., Duchaud E., Durant L., Dussurget O., Entian K.D., Fsihi H., García-del Portillo F., Garrido P., Gautier L., Goebel W., Gómez-López N., Hain T., Hauf J., Jackson D., Jones L.M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Vicente J.M., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Pérez-Diaz J.C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vázquez-Boland J.A., Voss H., Wehland J., Cossart P. Comparative genomics of Listeria species. Science 2001, 294:849-852.
18. Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S., Shiba T., Hattori M., Shinagawa H. Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with laboratory strain K-12. DNA Research 2001, 28:11-22.
19. Hoorfar J., Ahrens P., Radström P. Automated 5'nuclease PCR assay for identification of Salmonella enterica. Journal of Clinical Microbiology 2000, 38:3429-3435.
20. - serotype. Applied and Environmental Microbiology 2003, 69:6327-6333.
21. Johnston L.M., Elhanafi D., Drake M., Jaykus L.A. A simple method for the direct detection of Salmonella and Escherichia coli O157:H7 from raw alfalfa sprouts and spent irrigation water using PCR. Journal of Food Protection 2005, 68:2256-2263.
22. Kang D.H., Dougherty R.H., Fung D.Y.C. Comparison of pulsifier and stomacher to detach microorganisms from lean meat tissues. Journal of Rapid Methods and Automation in Microbiology 2001, 9:27-32.
23. Kim H.Y., Park H.J., Ko G. Hollow-fiber ultrafiltration for the concentration and simultaneous recovery of multiple pathogens in contaminated foods. Journal of Food Protection 2009, 72:2547-2552.
24. Lynch M.F., Tauxe R.V., Hedberg C.W. The growing burden of foodborne outbreaks due to contaminated fresh produce: risks and opportunities. Epidemiology and Infection 2009, 137:307-315.
25. Martínez-Blanch J.F., Sanchez G., Garay E., Aznar R. Development of a Real-Time PCR assay for detection and quantification of enterotoxigenic members of Bacillus cereus group in food samples. International Journal of Food Microbiology 2009, 135:15-21.
26. Mattison K., Harlow J., Morton V., Cook A., Pollari F., Bidawid S., Farber J.M. Enteric viruses in ready-to-eat packaged leafy greens. Emerging Infectious Diseases 2010, 16:1815-1817.
27. Maurer J.J. The mythology of PCR: a warning to the wise. PCR methods in foods 2006, 27-40. Springer, New York. J.J. Maurer (Ed.).
28. Mayrl E., Roeder B., Mester P., Wagner M., Rossmanith P. Broad range evaluation of the matrix solubilization (matrix lysis) strategy for direct enumeration of foodborne pathogens by nucleic acids technologies. Journal of Food Protection 2009, 72:1225-1233.
29. McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E., Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature 2001, 413:852-856.
30. Rodríguez-Lázaro D., Hernandez M., Scortti M., Esteve T., Boland-Vazquez J.A., Pla M. Quantitative detection of Listeria monocytogenes and Listeria innocua by Real-Time PCR: assessment of hly, iap, and lin02483: Targets and AmpliFluor Technology. Applied and Environmental Microbiology 2003, 70:1366-1377.
31. Rossmanith P., Suss B., Wagner M., Hein I. Development of matrix lysis for concentration of gram positive bacteria from food and blood. Journal of Microbiological Methods 2007, 69:504-511.
32. Shan X.C., Wolffs P., Griffiths M.W. Rapid and quantitative detection of hepatitis A virus from green onion and strawberry rinses by use of real-time reverse transcription-PCR. Applied and Environmental Microbiology 2005, 71:5624-5626.
33. Wu V., Jitareerat P., Fung D.Y.C. Comparison of the pulsifier and the stomacher for recovering microorganisms in vegetables. Journal of Rapid Methods and Automation in Microbiology 2003, 11:145-152.