High yield purification of nanobodies from the periplasm of E. coli as fusions with the maltose binding protein

Protein Expression and Purification

Volumn 91, Issue 1, 2013, Pages 42-48

  Salema, Valencio ^a   Fernández, Luis Ángel ^a  

^a CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

Affinity chromatography; E. coli; MBP; Nanobodies; Recombinant antibodies

Indexed keywords

ANTIGEN; ESCHERICHIA COLI PROTEIN; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; MALE PROTEIN, E COLI; NANOBODY; OLIGOPEPTIDE; PERIPLASMIC BINDING PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; MBP; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN BINDING; RECOMBINANT ANTIBODIES;

AFFINITY CHROMATOGRAPHY; E. COLI; MBP; NANOBODIES; RECOMBINANT ANTIBODIES;

AMINO ACID SEQUENCE; ANTIGENS; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HISTIDINE; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; PERIPLASMIC BINDING PROTEINS; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SINGLE-DOMAIN ANTIBODIES;

EID: 84880897111     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.07.001     Document Type: Article

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