Synthetic shuffling and in vitro selection reveal the rugged adaptive fitness landscape of a kinase ribozyme

RNA

Volumn 19, Issue 8, 2013, Pages 1116-1128

  Curtis, Edward A ^a,b,c   Bartel, David P ^a,b  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c HARVARD UNIVERSITY   (United States)

Author keywords

Fitness landscape; In vitro selection; Ribozyme; Synthetic recombination; Synthetic shuffling

Indexed keywords

NUCLEOTIDE; PHOSPHOTRANSFERASE; RIBOZYME;

ADAPTIVE FITNESS; ARTICLE; CHEMICAL PARAMETERS; GENE REARRANGEMENT; GENETIC RECOMBINATION; GENETIC VARIABILITY; IN VITRO SELECTION; IN VITRO STUDY; ISOLATION PROCEDURE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; PROTEIN SYNTHESIS; SYNTHESIS; SYNTHETIC SHUFFLING;

FITNESS LANDSCAPE; IN VITRO SELECTION; RIBOZYME; SYNTHETIC RECOMBINATION; SYNTHETIC SHUFFLING;

BASE SEQUENCE; DIRECTED MOLECULAR EVOLUTION; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID CONFORMATION; RECOMBINATION, GENETIC; RNA, CATALYTIC; THERMODYNAMICS;

EID: 84880673487     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.037572.112     Document Type: Article

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