Sulforaphane inhibits the engagement of LPS with TLR4/MD2 complex by preferential binding to Cys133 in MD2

Biochemical and Biophysical Research Communications

Volumn 434, Issue 3, 2013, Pages 600-605

  Koo, Jung Eun ^a   Park, Zee Yong ^b   Kim, Nam Doo ^c   Lee, Joo Young ^a  

^a The Catholic University of Korea   (South Korea)

^b Gwangju Institute of Science and Technology (GIST)   (South Korea)

^c Daegu Gyeongbuk Medical Innovation Foundation   (South Korea)

Author keywords

MD2; Phytochemical; Thiol; Toll like receptor 4

Indexed keywords

CYSTEINE; LIPOPOLYSACCHARIDE; PROTEIN MD 2; SULFORAPHANE; TOLL LIKE RECEPTOR 4; LY96 PROTEIN, MOUSE; SULFORAFAN; THIOCYANIC ACID DERIVATIVE; TLR4 PROTEIN, MOUSE;

ANIMAL CELL; ANTIINFLAMMATORY ACTIVITY; ARTICLE; BONE MARROW CELL; CONTROLLED STUDY; COVALENT BOND; DRUG MECHANISM; DRUG PROTEIN BINDING; LIQUID CHROMATOGRAPHY; MACROPHAGE; MOLECULAR MODEL; MOUSE; NONHUMAN; PRIORITY JOURNAL; TANDEM MASS SPECTROMETRY; AMINO ACID SEQUENCE; ANIMAL; C57BL MOUSE; CELL CULTURE; CHEMICAL STRUCTURE; CHEMISTRY; DRUG EFFECT; IMMUNOPRECIPITATION; METABOLISM; MOLECULAR GENETICS; PROTEIN BINDING; WESTERN BLOTTING;

IVA; NEGIBACTERIA;

AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CELLS, CULTURED; CHROMATOGRAPHY, LIQUID; IMMUNOPRECIPITATION; LIPOPOLYSACCHARIDES; LYMPHOCYTE ANTIGEN 96; MICE; MICE, INBRED C57BL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; TANDEM MASS SPECTROMETRY; THIOCYANATES; TOLL-LIKE RECEPTOR 4;

MLCS; MLOWN;

EID: 84880470388     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.03.123     Document Type: Article

References (18)

1. U. Ohto, K. Fukase, K. Miyake, Y. Satow, Crystal structures of human MD-2 and its complex with antiendotoxic lipid IVa, Science 316 (2007) 1632-1634.
2. R. Shimazu, S. Akashi, H. Ogata, Y. Nagai, K. Fukudome, K. Miyake, M. Kimoto, MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4, Jounal of Experimental Medicie 189 (1999) 1777-1782.
3. B.S. Park, D.H. Song, H.M. Kim, B.S. Choi, H. Lee, J.O. Lee, The structural basis of lipopolysaccharide recognition by the TLR4-MD-2 complex, Nature 458 (2009) 1191-1195.
4. T. Kawai, S. Akira, The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors, Nature Immunology 11 (2010) 373-384.
5. J.Y. Lee, L. Zhao, D.H. Hwang, Modulation of pattern recognition receptor-mediated inflammation and risk of chronic diseases by dietary fatty acids, Nutrition Reviews 68 (2010) 38-61.
6. D.S. Im, A promising anti-inflammatory and anti-thrombotic drug for sepsis treatment, Archives of Pharmacal Research 34 (2011) 339-342.
7. H.S. Youn, Y.S. Kim, Z.Y. Park, S.Y. Kim, N.Y. Choi, S.M. Joung, J.A. Seo, K.M. Lim, M.K. Kwak, D.H. Hwang, J.Y. Lee, Sulforaphane suppresses oligomerization of TLR4 in a thiol-dependent manner, Journal of Immunology 184 (2010) 411-419.
8. E. Heiss, C. Herhaus, K. Klimo, H. Bartsch, C. Gerhauser, Nuclear factor kappa B is a molecular target for sulforaphane-mediated anti-inflammatory mechanisms, Journal of Biological Chemistry 276 (2001) 32008-32015.
9. Y.C. Liu, C.W. Hsieh, Y.C. Weng, S.H. Chuang, C.Y. Hsieh, B.S. Wung, Sulforaphane inhibition of monocyte adhesion via the suppression of ICAM-1 and NF-kappaB is dependent upon glutathione depletion in endothelial cells, Vascular Pharmacology 48 (2008) 54-61.
10. W. Lin, R.T. Wu, T. Wu, T.O. Khor, H. Wang, A.N. Kong, Sulforaphane suppressed LPS-induced inflammation in mouse peritoneal macrophages through Nrf2 dependent pathway, Biochemical Pharmacology 76 (2008) 967-973.
11. S.Y. Kim, J.E. Koo, Y.J. Seo, N. Tyagi, E. Jeong, J. Choi, K.M. Lim, Z.Y. Park, J.Y. Lee, Suppression of Toll-like receptor 4 activation by caffeic acid phenethyl ester is mediated by interference of LPS binding to MD2, British Journal of Pharmacology (2012), http://dx.doi.org/10.1111/bph.12091.
12. Y. Nagai, S. Akashi, M. Nagafuku, M. Ogata, Y. Iwakura, S. Akira, T. Kitamura, A. Kosugi, M. Kimoto, K. Miyake, Essential role of MD-2 in LPS responsiveness and TLR4 distribution, Nature Immunology 3 (2002) 667-672.
13. S. Hadina, J.P. Weiss, P.B. McCray Jr., K. Kulhankova, P.S. Thorne, MD-2-dependent pulmonary immune responses to inhaled lipooligosaccharides: effect of acylation state, American Journal of Respiratory Cell Molecular Biology 38 (2008) 647-654.
14. H.M. Kim, B.S. Park, J.I. Kim, S.E. Kim, J. Lee, S.C. Oh, P. Enkhbayar, N. Matsushima, H. Lee, O.J. Yoo, J.O. Lee, Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran, Cell 130 (2007) 906-917.
15. S.H. Park, N.D. Kim, J.K. Jung, C.K. Lee, S.B. Han, Y. Kim, Myeloid differentiation 2 as a therapeutic target of inflammatory disorders, Pharmacology Therapeutics 133 (2012) 291-298.
16. H. Gradisar, M.M. Keber, P. Pristovsek, R. Jerala, MD-2 as the target of curcumin in the inhibition of response to LPS, Journal of Leukocyte Biology 82 (2007) 968-974.
17. F. Re, J.L. Strominger, Separate functional domains of human MD-2 mediate Toll-like receptor 4-binding and lipopolysaccharide responsiveness, Journal of Immunology 171 (2003) 5272-5276.
18. G.E. Mullen, M.N. Kennedy, A. Visintin, A. Mazzoni, C.A. Leifer, D.R. Davies, D.M. Segal, The role of disulfide bonds in the assembly and function of MD-2, Proceedings of National Academy of Sciences USA 100 (2003) 3919-3924.