Measuring mitochondrial uncoupling protein-2 level and activity in insulinoma cells

Methods in Enzymology

Volumn 528, Issue , 2013, Pages 257-267

  Barlow, Jonathan ^a   Hirschberg, Verena ^a   Brand, Martin D ^b   Affourtit, Charles ^a  

^a UNIVERSITY OF PLYMOUTH   (United Kingdom)

^b BUCK INSTITUTE FOR RESEARCH ON AGING   (United States)

Author keywords

(UCP2) UCP2 detection and knockdown; Cellular bioenergetics; Coupling efficiency of oxidative phosphorylation; Glucose stimulated insulin secretion; Insulinoma cells; Mitochondrial uncoupling protein 2; Pancreatic beta cells; Reactive oxygen species; Type 2 diabetes; UCP2 activity

Indexed keywords

GLUCOSE; INSULIN; REACTIVE OXYGEN METABOLITE; UNCOUPLING PROTEIN 2;

ANIMAL CELL; ARTICLE; CELL DENSITY; CELL RESPIRATION; CONTROLLED STUDY; GENE SILENCING; INSULIN RELEASE; INSULINOMA; MITOCHONDRION; MOUSE; NONHUMAN; OXIDATIVE PHOSPHORYLATION; PHENOTYPE; PRIORITY JOURNAL; RNA INTERFERENCE; TUMOR CELL;

EID: 84880291002     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-405881-1.00015-X     Document Type: Chapter

References (10)

1. C. Affourtit, and M.D. Brand Measuring mitochondrial bioenergetics in INS-1E insulinoma cells Methods in Enzymology 457 2009 405 424
2. C. Affourtit, M. Jastroch, and M.D. Brand Uncoupling protein-2 attenuates glucose-stimulated insulin secretion in INS-1E insulinoma cells by lowering mitochondrial reactive oxygen species Free Radical Biology & Medicine 50 2011 609 616
3. J. Barlow, V. Hirschberg, and C. Affourtit On the role of mitochondria in pancreatic beta cells Research on diabetes 2013 (edited by: i.Press) iConcept Press. ISBN: 978-14775550-1-9
4. T.C. Esteves, and M.D. Brand The reactions catalysed by the mitochondrial uncoupling proteins UCP2 and UCP3 Biochimica et Biophysica Acta 1709 2005 35 44
5. A.A. Gerencser, C. Chinopoulos, M.J. Birket, M. Jastroch, C. Vitelli, and D.G. Nicholls Quantitative measurement of mitochondrial membrane potential in cultured cells: Calcium-induced de- and hyperpolarization of neuronal mitochondria The Journal of Physiology 590 2012 2845 2871
6. M.J. MacDonald Perspective: Emerging evidence for signaling roles of mitochondrial anaplerotic products in insulin secretion American Journal of Physiology. Endocrinology and Metabolism 288 2004 E1 E15
7. J. Pi, Y. Bai, Q. Zhang, V. Wong, L.M. Floering, and K. Daniel Reactive oxygen species as a signal in glucose-stimulated insulin secretion Diabetes 56 2007 1783 1791
8. C.A. Robson-Doucette, S. Sultan, E.M. Allister, J.D. Wikstrom, V. Koshkin, and A. Bhattacharjee Uncoupling protein-2 regulates reactive oxygen species production, which influences both insulin and glucagon secretion Diabetes 60 2011 2710 2719
9. G.A. Rutter Nutrient-secretion coupling in the pancreatic islet beta-cell: Recent advances Molecular Aspects of Medicine 22 2001 247 284
10. C.-Y. Zhang, G. Baffy, P. Perret, S. Krauss, O. Peroni, and D. Grujic Uncoupling protein-2 negatively regulates insulin secretion and is a major link between obesity, beta cell dysfunction, and type 2 diabetes Cell 105 2001 745 755