Chapter 23 Measuring Mitochondrial Bioenergetics in INS-1E Insulinoma Cells

Methods in Enzymology

Volumn 457, Issue B, 2009, Pages 405-424

  Affourtit, Charles ^a   Brand, Martin D ^a,b  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b BUCK INSTITUTE FOR RESEARCH ON AGING   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBONYL CYANIDE 4 (TRIFLUOROMETHOXY)PHENYLHYDRAZONE; OLIGOMYCIN; TRYPSIN; UNCOUPLING PROTEIN 2; ION CHANNEL; MITOCHONDRIAL PROTEIN; MITOCHONDRIAL UNCOUPLING PROTEIN 2;

ANIMAL CELL; BIOENERGY; CELL LYSATE; CELL STRAIN INS 1E; CONTROLLED STUDY; GRANULE CELL; INSULINOMA CELL; LIVER CELL; MITOCHONDRIAL ENERGY TRANSFER; MITOCHONDRIAL RESPIRATION; MYOBLAST; NONHUMAN; OXIDATIVE PHOSPHORYLATION; OXYGEN CONSUMPTION; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTON MOTIVE FORCE; RAT; REVIEW; RNA INTERFERENCE; THYMOCYTE; TISSUE CULTURE; TUMOR CELL LINE; WESTERN BLOTTING; ANIMAL; ARTICLE; CHEMISTRY; ENERGY METABOLISM; GENE SILENCING; GENETICS; HUMAN; INSULINOMA; KINETICS; METABOLISM; MITOCHONDRION;

ANIMALS; CELL LINE, TUMOR; ENERGY METABOLISM; GENE KNOCKDOWN TECHNIQUES; HUMANS; INSULIN-SECRETING CELLS; INSULINOMA; ION CHANNELS; KINETICS; MITOCHONDRIA; MITOCHONDRIAL PROTEINS; OXYGEN CONSUMPTION; RNA INTERFERENCE;

EID: 65449174289     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(09)05023-X     Document Type: Review

References (33)

1. Affourtit C., and Brand M.D. Stronger control of ATP/ADP by proton leak in pancreatic beta-cells than skeletal muscle mitochondria. Biochem. J. 393 (2006) 151-159
2. Affourtit C., and Brand M. On the role of uncoupling protein-2 in pancreatic beta cells. Biochim. Biophys. Acta 1777 (2008) 973-979
3. Affourtit C., and Brand M.D. Uncoupling protein-2 contributes significantly to high mitochondrial proton leak in INS-1E insulinoma cells and attenuates glucose-stimulated insulin secretion. Biochem. J. 409 (2008) 199-204
4. Amo T., Yadava N., Oh R., Nicholls D.G., and Brand M.D. Experimental assessment of bioenergetic differences caused by the common European mitochondrial DNA haplogroups H and T. Gene 411 (2008) 69-76
5. Asfari M., Janjic D., Meda P., Li G., Halban P.A., and Wollheim C.B. Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines. Endocrinology 130 (1992) 167-178
6. Azzu V., Affourtit C., Breen E., Parker N., and Brand M.D. Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells. Biochim. Biophys. Acta 1770 (2008) 1378-1383
7. Bishop T., and Brand M.D. Processes contributing to metabolic depression in hepatopancreas cells from the snail Helix aspersa. J. Exp. Biol. 203 (2000) 3603-3612
8. Brand M.D. The proton leak across the mitochondrial inner membrane. Biochim. Biophys. Acta 1018 (1990) 128-133
9. Brand M.D. Top-down elasticity analysis and its application to energy metabolism in isolated mitochondria and intact cells. Mol. Cell. Biochem. 184 (1998) 13-20
10. Brand M.D., Affourtit C., Amo T., Choi S.W., Cornwall E.J., Gerencser A.A., Nicholls D.G., Oh R., Parker N., and Yadava N. The mechanisms, regulation and functions of mitochondrial uncoupling. In: Morris S., and Vosloo A. (Eds). 4th CPB Meeting in Africa: Mara 2008. Molecules to migration: The pressures of life (2008), Medimond publishing Co, Bologna, Italy 331-338 via Maserati 6/2, 40124
11. Brand M.D., Bishop T., Boutilier R.G., and St-Pierre J. Mitochondrial proton conductance, standard metabolic rate and metabolic depression. In: Heldmaier G., and Klingenspor M. (Eds). Life in the Cold (2000), Springer, Berlin 413-430
12. Brand M.D., Couture P., Else P.L., Withers K.W., and Hulbert A.J. Evolution of energy metabolism. Proton permeability of the inner membrane of liver mitochondria is greater in a mammal than in a reptile. Biochem. J. 275 (1991) 81-86
13. Brown G.C., Lakin-Thomas P.L., and Brand M.D. Control of respiration and oxidative phosphorylation in isolated rat liver cells. Eur. J. Biochem. 192 (1990) 355-362
14. Buttgereit F., Grant A., Müller M., and Brand M.D. The effects of methylprednisolone on oxidative phosphorylation in Concanavalin-A-stimulated thymocytes. Top-down elasticity analysis and control analysis. Eur. J. Biochem. 223 (1994) 513-519
15. Else P.L., Brand M.D., Turner N., and Hulbert A.J. Respiration rate of hepatocytes varies with body mass in birds. J. Exp. Biol. 207 (2004) 2305-2311
16. Harper M.E., and Brand M.D. The quantitative contributions of mitochondrial proton leak and ATP turnover reactions to the changed respiration rates of hepatocytes from rats of different thyroid status. J. Biol. Chem. 268 (1993) 14850-14860
17. Harper M.E., and Brand M.D. Use of top-down elasticity analysis to identify sites of thyroid hormone-induced thermogenesis. Proc. Soc. Exp. Biol. Med. 208 (1995) 228-237
18. Harrison R. Human xanthine oxidoreductase: In search of a function. Biochem. Soc. Trans. 25 (1997) 786-791
19. Hulbert A.J., Else P.L., Manolis S.C., and Brand M.D. Proton leak in hepatocytes and liver mitochondria from archosaurs (crocodiles) and allometric relationships for ectotherms. J. Comp. Physiol. B. Biochem. Syst. Environ. Physiol. 172 (2002) 387-397
20. Janjic D., Maechler P., Sekine N., Bartley C., Annen A.S., and Wolheim C.B. Free radical modulation of insulin release in INS-1 cells exposed to alloxan. Biochem. Pharmacol. 57 (1999) 639-648
21. Jekabsons M.B., and Nicholls D.G. In situ respiration and bioenergetic status of mitochondria in primary cerebellar granule neuronal cultures exposed continuously to glutamate. J. Biol. Chem. 279 (2004) 32989-33000
22. Merglen A., Theander S., Rubi B., Chaffard G., Wollheim C.B., and Maechler P. Glucose sensitivity and metabolism-secretion coupling studied during two-year continuous culture in INS-1E insulinoma cells. Endocrinology 145 (2004) 667-678
23. Nicholls D.G. Simultaneous monitoring of ionophore- and inhibitor-mediated plasma and mitochondrial membrane potential changes in cultured neurons. J. Biol. Chem. 281 (2006) 14864-14874
24. Nobes C.D., Brown G.C., Olive P.N., and Brand M.D. Non-ohmic proton conductance of the mitochondrial inner membrane in hepatocytes. J. Biol. Chem. 265 (1990) 12903-12909
25. Porter R.K., and Brand M.D. Causes of differences in respiration rate of hepatocytes from mammals of different body mass. Am. J. Physiol. 269 (1995) R1213-R1224
26. 2 solubility in aqueous media determined by a kinetic method. Anal. Biochem. 145 (1985) 406-418
27. Rolfe D.F.S., Newman J.M., Buckingham J.A., Clark M.G., and Brand M.D. Contribution of mitochondrial proton leak to respiration rate in working skeletal muscle and liver and to SMR. Am. J. Physiol. 276 (1999) C692-C699
28. Rutter G.A. Nutrient-secretion coupling in the pancreatic islet beta-cell: Recent advances. Mol. Aspects Med. 22 (2001) 247-284
29. Savina M.V., and Gamper N.L. Respiration and adenine nucleotides of Baltic lamprey (Lampetra fluviatilis l.) hepatocytes during spawning migration. Comp. Biochem. Phys. B. Biochem. Mol. Biol. 120 (1998) 375-383
30. Sweet I.R., Li G., Najafi H., Berner D., and Matschinsky F.M. Effect of a glucokinase inhibitor on energy production and insulin release in pancreatic islets. Am. J. Physiol. 271 (1996) E606-E625
31. van den Bosch H., Schutgens R.B., Wanders R.J., and Tager J.M. Biochemistry of peroxisomes. Annu. Rev. Biochem. 61 (1992) 157-197
32. Wu M., Neilson A., Swift A.L., Moran R., Tamagnine J., Parslow D., Armistead S., Lemire K., Orrell J., Teich J., Chomicz S., and Ferrick D.A. Multiparameter metabolic analysis reveals a close link between attenuated mitochondrial bioenergetic function and enhanced glycolysis dependency in human tumor cells. Am. J. Physiol. Cell Physiol. 292 (2007) C125-C136
33. Zhang C.Y., Baffy G., Perret P., Krauss S., Peroni O., Grujic D., Hagen T., Vidal-Puig A.J., Boss O., Kim Y.B., Zheng X.X., Wheeler M.B., et al. Uncoupling protein-2 negatively regulates insulin secretion and is a major link between obesity, beta cell dysfunction, and type 2 diabetes. Cell 105 (2001) 745-755