Enhancement of antibody fragment secretion into the Escherichia coli periplasm by co-expression with the peptidyl prolyl isomerase, FkpA, in the cytoplasm

Journal of Immunological Methods

Volumn 394, Issue 1-2, 2013, Pages 10-21

  Levy, Raphael ^a   Ahluwalia, Kiran ^a   Bohmann, David J ^a   Giang, Hoa M ^a   Schwimmer, Lauren J ^a   Issafras, Hassan ^a   Reddy, Nithin B ^a   Chan, Chung ^a   Horwitz, Arnold H ^a   Takeuchi, Toshihiko ^a  

^a XOMA Corporation   (United States)

Author keywords

Bacteria; Chaperones; Cytoplasm; Phage display

Indexed keywords

EPITHELIAL CELL ADHESION MOLECULE; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN KAPPA CHAIN; IMMUNOGLOBULIN LAMBDA CHAIN; PEPTIDYL PROLYL ISOMERASE FKPA; PEPTIDYLPROLYL ISOMERASE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TIE 1 RECEPTOR; UNCLASSIFIED DRUG;

ANTIBODY PRODUCTION; ANTIBODY SCREENING; ANTIGEN BINDING; ARTICLE; BACTERIOPHAGE; CONTROLLED STUDY; CYTOPLASM; CYTOSOL; ESCHERICHIA COLI; ISOMERIZATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION;

3,3′,5,5′-TETRAMETHYLBENZIDINE; BACTERIA; BOVINE SERUM ALBUMIN; BSA; CFU; CHAPERONES; COLONY-FORMING UNITS; CYTOPLASM; DISSOCIATION CONSTANT; EC(50); ELISA; ENZYME-LINKED IMMUNOSORBENT ASSAY; FAB; FC; FD; FRAGMENT CRYSTALLIZABLE; FRAGMENT OF ANTIBODY CONSISTING OF CH1 AND VH; FRAGMENT-ANTIGEN-BINDING; HALF MAXIMAL EFFECTIVE CONCENTRATION; HORSERADISH PEROXIDASE; HRP; HUINSR; HUMAN INSULIN RECEPTOR; IPTG; ISOPROPYL Β-D-1-THIOGALACTOPYRANOSIDE; KD; M.O.I.; MULTIPLICITY OF INFECTION; OD; OPTICAL DENSITY; PBS; PCR; PEG; PEPTIDYL PROLYL CIS-TRANS ISOMERASE; PHAGE DISPLAY; PHOSPHATE BUFFER SALINE; POLYETHYLENE GLYCOL; POLYMERASE CHAIN REACTION; POLYVINYLIDENE FLUORIDE; PPIASE; PVDF; ROOM TEMPERATURE; RT; SCFV; SD; SDS; SEM; SHINE-DALGARNO SEQUENCE; SINGLE-CHAIN VARIABLE FRAGMENT; SODIUM DODECYL SULFATE; SPR; STANDARD ERROR OF THE MEAN; SURFACE PLASMON RESONANCE; TMB; VARIABLE HEAVY; VARIABLE LIGHT; VH; VL;

ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; IMMUNOGLOBULIN FAB FRAGMENTS; MEMBRANE PROTEINS; PEPTIDE LIBRARY; PEPTIDYLPROLYL ISOMERASE; PERIPLASM; PROTEIN FOLDING;

ESCHERICHIA COLI;

EID: 84880046031     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.04.010     Document Type: Article

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