Functional expression of single-chain variable fragment antibody against c-Met in the cytoplasm of Escherichia coli

Protein Expression and Purification

Volumn 47, Issue 1, 2006, Pages 203-209

  Heo, Mi Ae ^a   Kim, Su Hyun ^a   Kim, So Yeon ^a   Kim, Yu Jin ^a   Chung, Junho ^b   Oh, Min Kyu ^c   Lee, Sun Gu ^a  

^a Pusan National University   (South Korea)

^b Seoul National University College of Medicine   (South Korea)

^c Korea University   (South Korea)

Author keywords

Anti c Met scFv; Folding; Molecular chaperones; Soluble expression

Indexed keywords

ANTIBODY; IMMUNOGLOBULIN FRAGMENT; SCATTER FACTOR RECEPTOR;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; CYTOPLASM; ESCHERICHIA COLI; GENETICS; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; MOLECULAR CLONING; PHYSIOLOGY;

ANTIBODIES; CLONING, MOLECULAR; CYTOPLASM; ESCHERICHIA COLI; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; PROTO-ONCOGENE PROTEINS C-MET;

ESCHERICHIA COLI;

EID: 33646077190     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.12.003     Document Type: Article

References (21)

1. Maulik G., Shrikhande A., Kijima T., Ma P.C., Morrison P.T., and Salgia R. Role of the hepatocyte growth factor, c-Met, in oncogenesis and potential for therapeutic inhibition. Cytokine Growth Factor Rev. 13 (2002) 41-59
2. Jiang W.G., Martin T.A., Parr C., Davies G., Matsumoto K., and Nakamura T. Hepatocyte growth factor, its receptor, and their potential value in cancer therapies. Crit. Rev. Oncol. Hematol. 53 (2005) 35-69
3. Anderson D.C., and Reily D.E. Production technologies for monoclonal antibodies and their fragments. Curr. Opin. Biotechnol. 15 (2004) 456-462
4. Fernandez L.A. Prokaryotic expression of antibodies and affibodies. Curr. Opin. Biotechnol. 15 (2004) 364-373
5. Yi K.S., Chung J., Park K., Kim K., Im S., Choi C., Im M., and Kim U. Expression system for enhanced green fluorescence protein conjugated recombinant antibody fragment. Hybrid. Hybridomics 23 (2004) 279-286
6. Jurado P., Ritz D., Beckwith J., Lorenzo V., and Fernandez L.A. Production of functional single-chain Fv antibodies in the cytoplasm of E. coli. J. Mol. Biol. 320 (2002) 1-10
7. Choi G., Lee D., Min W., Cho Y., Kweon D., Son D., Park K., and Seo J. Cloning, expression, and characterization of single-chain variable fragment antibody against mycotoxin deoxynivalenol in recombinant E. coli. Protein Expr. Purif. 35 (2004) 84-92
8. Tavladoraki P., Girotti A., Donini M., Arias F.J., Mancini C., Morea V., Chiaraluce R., Consalvi V., and Benvenuto E. A single-chain antibody fragment is functionally expressed in the cytoplasm of both Escherichia coli and transgenic plants. Eur. J. Biochem. 262 (1999) 617-624
9. Baneyx F., and Mujacic M. Recombinant protein folding and misfolding in E. coli. Nat. Biotechnol. 22 (2004) 1399-1408
10. Lee S., Lee J., Yi J., and Kim B. Production of cytidine 5-monophosphate N-acetylneuraminic acid using recombinant E. coli as a biocatalyst. Biotechnol. Bioeng. 80 (2002) 516-524
11. Sambrook J., and Russell D.W. Molecular Cloning. third ed. (2001), Cold Spring Harbor Laboratory Press, New York
12. Bessette P.H., Aslund F., Beckwith J., and Georgiou G. Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm. Proc. Natl. Acad. Sci. USA 96 (1999) 13703-13708
13. Lee K., Kim H., Jeong H., and Lee Y. Chaperonin GroELS mediates the protein folding of human liver mitochondrial aldehyde dehydrogenase in Escherichia coli. Biochem. Biophys. Res. Commun. 298 (2002) 216-224
14. Nishihara K., Kanemori M., Kitakawa M., Yanaki H., and Yura T. Chaperone coexpression plasmids: differential and synergenic roles of DnaK-DnaJ-GrpE and GroEL-GroES in assisting folding of an allergen of Japanese cedar pollen, Cryj2, in E. coli. Appl. Environ. Microbiol. 64 (1998) 1694-1699
15. Nishihara K., Kanemori M., Kitakawa M., Yanaki H., and Yura T. Overexpression of trigger factor prevents aggregation of recombinant proteins in E. coli. Appl. Environ. Microbiol. 66 (2000) 884-889
16. Levy R., Weiss R., Chen G., Iverson B.L., and Georgiou G. Production of correctly folded Fab antibody fragment in the cytoplasm of Escherichia coli trxB gor mutants via the coexpression of molecular chaperones. Protein Expr. Purif. 23 (2001) 338-347
17. Baneyx F. Recombinant protein expression in Escherichia coli. Curr. Opin. Biotechnol. 10 (1999) 411-421
18. Vasina J.A., and Baneyx F. Expression of aggregation-prone recombinant proteins at low temperatures: a comparative study of the Escherichia coli cspA and tac promoter systems. Protein Expr. Purif. 9 (1997) 211-218
19. Barth S., Huhn M., Matthey B., Klimka A., Galinski E.A., and Engert A. Compatible-solute-supported periplasmic expression of functional recombinant proteins under stress conditions. Appl. Environ. Microbiol. 66 (2000) 1572-1579
20. Sandee D., Tungpradabkul S., Kurokawa Y., Fukui K., and Takagi M. Combination of DsbC coexpression and an addition of sorbitol markedly enhanced soluble expression of single-chain Fv in Escherichia coli. Biotechnol. Bioeng. 91 (2005) 418-424
21. Diamant S., Eliahu N., Rosenthal D., and Goloubinoff P. Chemical chaperones regulate molecular chaperones in vitro and in cells under combined salt and heat stresses. J. Biol. Chem. 276 (2001) 39586-39591