A Dual-Mode Surface Display System for the Maturation and Production of Monoclonal Antibodies in Glyco-Engineered Pichia pastoris

PLoS ONE

Volumn 8, Issue 7, 2013, Pages

  Shaheen, Hussam H ^a   Prinz, Bianka ^a,b   Chen, Ming Tang ^a   Pavoor, Tej ^a,c   Lin, Song ^a   Houston Cummings, Nga Rewa ^a   Moore, Renee ^a   Stadheim, Terrance A ^a   Zha, Dongxing ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b Adimab   (United States)

^c NONE

Author keywords

[No Author keywords available]

Indexed keywords

GLYCAN DERIVATIVE; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; MONOCLONAL ANTIBODY;

ARTICLE; BIOENGINEERING; BIOTECHNOLOGICAL PRODUCTION; CELL SURFACE DISPLAY; CELLULAR SECRETION; CONTROLLED STUDY; DIMERIZATION; DRUG RESEARCH; DRUG SYNTHESIS; FUNGAL CELL WALL; FUNGAL REPRODUCTION; GLYCOENGINEERING; HOMODIMERIZATION; MOLECULAR LIBRARY; NONHUMAN; PICHIA PASTORIS; YEAST CELL;

ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; ANTIBODY FORMATION; CELL SEPARATION; GLYCOSYLATION; HUMANS; ORGANISMS, GENETICALLY MODIFIED; PEPTIDE LIBRARY; PICHIA; PROTEIN ENGINEERING; PROTEIN MULTIMERIZATION; PROTEIN PROCESSING, POST-TRANSLATIONAL;

EUKARYOTA; PICHIA PASTORIS; PROKARYOTA;

EID: 84880020525     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0070190     Document Type: Article

References (31)

1. Rakestraw JA, Aird D, Aha PM, Baynes BM, Lipovsek D, (2011) Secretion-and-capture cell-surface display for selection of target-binding proteins. Protein Eng Des Sel 24: 525-530. doi:10.1093/protein/gzr008. PubMed: 21402751.
2. McCafferty J, Griffiths AD, Winter G, Chiswell DJ, (1990) Phage antibodies: filamentous phage displaying antibody variable domains. Nature 348: 552-554. doi:10.1038/348552a0. PubMed: 2247164.
3. Lin S, Houston-Cummings NR, Prinz B, Moore R, Bobrowicz B, et al. (2011) A novel fragment of antigen binding (Fab) surface display platform using glycoengineered Pichia pastoris. J Immunol Methods 31;375: 1-2:159-65.
4. Lee CV, Sidhu SS, Fuh G, (2004) Bivalent antibody phage display mimics natural immunoglobulin. J Immunol Methods 284: 119-132. doi:10.1016/j.jim.2003.11.001. PubMed: 14736422.
5. Hoogenboom HR, Griffiths AD, Johnson KS, Chiswell DJ, Hudson P, et al. (1991) Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res 19: 4133-4137. doi:10.1093/nar/19.15.4133. PubMed: 1908075.
6. Bender E, Woof JM, Atkin JD, Barker MD, Bebbington CR, et al. (1993) Recombinant human antibodies: linkage of an Fab fragment from a combinatorial library to an Fc fragment for expression in mammalian cell culture. Hum Antibodies Hybridomas 4: 74-79.
7. Liang M, Dübel S, Li D, Queitsch I, Li W, et al. (2001) Baculovirus expression cassette vectors for rapid production of complete human IgG from phage display selected antibody fragments. J Immunol Methods 247: 119-130. doi:10.1016/S0022-1759(00)00322-7. PubMed: 11150543.
8. Mazor Y, Van Blarcom T, Mabry R, Iverson BL, Georgiou G, (2007) Isolation of engineered, full-length antibodies from libraries expressed in Escherichia coli. Nat Biotechnol 25: 563-565. doi:10.1038/nbt1296. PubMed: 17435747.
9. Zhou C, Jacobsen FW, Cai L, Chen Q, Shen WD, (2010) Development of a novel mammalian cell surface antibody display platform. MAbs 2: 508-518. doi:10.4161/mabs.2.5.12970. PubMed: 20716968.
10. Harvey BR, Georgiou G, Hayhurst A, Jeong KJ, Iverson BL, et al. (2004) Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries. Proc Natl Acad Sci U S A 101: 9193-9198. doi:10.1073/pnas.0400187101. PubMed: 15197275.
11. Harvey BR, Shanafelt AB, Baburina I, Hui R, Vitone S, et al. (2006) Engineering of recombinant antibody fragments to methamphetamine by anchored periplasmic expression. J Immunol Methods 308: 43-52. doi:10.1016/j.jim.2005.09.017. PubMed: 16337958.
12. Rani M, Bolles M, Donaldson EF, Van Blarcom T, Baric R, et al. (2012) Increased antibody affinity confers broad in vitro protection against escape mutants of severe acute respiratory syndrome coronavirus. J Virol 86: 9113-9121. doi:10.1128/JVI.00233-12. PubMed: 22696652.
13. Ryckaert S, Pardon E, Steyaert J, Callewaert N, (2010) Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris. J Biotechnol 145: 93-98. doi:10.1016/j.jbiotec.2009.10.010. PubMed: 19861136.
14. Shibasaki S, Kawabata A, Ishii J, Yagi S, Kadonosono T, et al. (2007) Construction of a novel synergistic system for production and recovery of secreted recombinant proteins by the cell surface engineering. Appl Microbiol Biotechnol 75: 821-828. doi:10.1007/s00253-007-0868-1. PubMed: 17345082.
15. Rakestraw JA, Baskaran AR, Wittrup KD, (2006) A flow cytometric assay for screening improved heterologous protein secretion in yeast. Biotechnol Prog 22: 1200-1208. doi:10.1021/bp0600233. PubMed: 16889399.
16. Horlick RA, Macomber JL, Bowers PM, Neben TY, Tomlinson GL, et al. (2013) Simultaneous Surface Display and Secretion of Proteins from Mammalian Cells Facilitates Efficient in vitro Selection and Maturation of Antibodies. J Biol Chem. PubMed: 23689374.
17. Li H, Sethuraman N, Stadheim TA, Zha D, Prinz B, et al. (2006) Optimization of humanized IgGs in glycoengineered Pichia pastoris. Nat Biotechnol 24: 210-215. doi:10.1038/nbt1178. PubMed: 16429149.
18. Zhang N, Liu L, Dumitru CD, Cummings NR, Cukan M, et al. (2011) Glycoengineered Pichia produced anti-HER2 is comparable to trastuzumab in preclinical study. MAbs 3: 289-298. doi:10.4161/mabs.3.3.15532. PubMed: 21487242.
19. Ye JX, Ly J, Watts K, Hsu A, Walker A, et al. (2011) Optimization of a glycoengineered Pichia pastoris cultivation process for commercial antibody production. Biotechnol Prog 27: 1744-1750. doi:10.1002/btpr.695. PubMed: 22002933.
20. Berdichevsky M, d'Anjou M, Mallem MR, Shaikh SS, Potgieter TI, (2011) Improved production of monoclonal antibodies through oxygen-limited cultivation of glycoengineered yeast. J Biotechnol 155: 217-224. doi:10.1016/j.jbiotec.2011.06.021. PubMed: 21723345.
21. Choi BK, Bobrowicz P, Davidson RC, Hamilton SR, Kung DH, et al. (2003) Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris. Proc Natl Acad Sci U S A 100: 5022-5027. doi:10.1073/pnas.0931263100. PubMed: 12702754.
22. Hamilton SR, Davidson RC, Sethuraman N, Nett JH, Jiang Y, Rios S, et al. (2006) Humanization of yeast to produce complex terminally sialylated glycoproteins. Science 313: 1441-1443. doi:10.1126/science.1130256. PubMed: 16960007.
23. Nett JH, Hodel N, Rausch S, Wildt S, (2005) Cloning and disruption of the Pichia pastoris ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and their use as auxotrophic markers. Yeast 22: 295-304. doi:10.1002/yea.1202. PubMed: 15789348.
24. Nett JH, Stadheim TA, Li H, Bobrowicz P, Hamilton SR, et al. (2011) A combinatorial genetic library approach to target heterologous glycosylation enzymes to the endoplasmic reticulum or the Golgi apparatus of Pichia pastoris. Yeast 28: 237-252. doi:10.1002/yea.1835. PubMed: 21360735.
25. Chen MT, Lin S, Shandil I, Andrews D, Stadheim TA, et al. (2012) Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production. Microb Cell Factories 11: 91. doi:10.1186/1475-2859-11-91. PubMed: 22748191.
26. Jiang YW, Li F, Zha DX, Potgieter TI, Mitchell T, et al. (2011) Purification process development of a recombinant monoclonal antibody expressed in glycoengineered Pichia pastoris. Protein Expr Purif 76: 7-14. doi:10.1016/j.pep.2010.11.004. PubMed: 21074617.
27. Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, et al. (2006) Isolating and engineering human antibodies using yeast surface display. Nat Protoc 1: 755-768. doi:10.1038/nprot.2006.94. PubMed: 17406305.
28. Kayser V, Chennamsetty N, Voynov V, Forrer K, Helk B, et al. (2011) Glycosylation influences on the aggregation propensity of therapeutic monoclonal antibodies. Biotechnol J 6: 38-44. doi:10.1002/biot.201000091. PubMed: 20949542.
29. Houde D, Peng YC, Berkowitz SA, Engen JR, (2010) Post-translational Modifications Differentially Affect IgG1 Conformation and Receptor Binding. Mol Cell Proteomics 9: 1716-1728. doi:10.1074/mcp.M900540-MCP200. PubMed: 20103567.
30. Jefferis R, (2010) The antibody paradigm: present and future development as a scaffold for biopharmaceutical drugs. Biotechnol Genet Eng Rev, Vol 2626:: 1-41. PubMed: 21415874.
31. Liu HC, Bulseco GG, Sun JA, (2006) Effect of posttranslational modifications on the thermal stability of a recombinant monoclonal antibody. Immunol Lett 106: 144-153. doi:10.1016/j.imlet.2006.05.011. PubMed: 16831470.