Simultaneous surface display and secretion of proteins from mammalian cells facilitate efficient in vitro selection and maturation of antibodies

Journal of Biological Chemistry

Volumn 288, Issue 27, 2013, Pages 19861-19869

  Horlick, Robert A ^a   MacOmber, John L ^a   Bowers, Peter M ^a   Neben, Tamlyn Y ^a   Tomlinson, Geoffery L ^a   Krapf, Irina P ^a   Dalton, Jennifer L ^a   Verdino, Petra ^a   King, David J ^a  

^a AnaptysBio Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE DISPLAYS; EXPRESSION SYSTEM; FUNCTIONAL ASSAYS; PROTEIN EVOLUTION; SECRETED PROTEIN; SOLUBLE PROTEINS; SOMATIC HYPERMUTATION; SURFACE DISPLAYS;

CELL MEMBRANES; PHYSIOLOGY;

ANTIBODIES;

HYBRID PROTEIN; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G ANTIBODY; MONOMER;

ALTERNATIVE RNA SPLICING; ANTIBODY PRODUCTION; ANTIGEN EXPRESSION; ARTICLE; BINDING AFFINITY; CELL MATURATION; CELL SELECTION; CELL SURFACE DISPLAY; COMPLEMENT ALTERNATIVE PATHWAY; CONTROLLED STUDY; DNA RECOMBINATION; HUMAN; HUMAN CELL; IN VITRO STUDY; MAMMAL CELL; PRIORITY JOURNAL; PROTEIN SECRETION; RNA BINDING; RNA SPLICING;

AFFINITY MATURATION; ALTERNATIVE SPLICING; ANTIBODIES; ANTIBODY ENGINEERING; MUTAGENESIS; PROTEIN DISPLAY; PROTEIN EVOLUTION; SOMATIC HYPERMUTATION;

ALTERNATIVE SPLICING; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; DIRECTED MOLECULAR EVOLUTION; GENE EXPRESSION; HEK293 CELLS; HUMANS; IMMUNOGLOBULIN G; RNA PRECURSORS;

EID: 84879997146     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M113.452482     Document Type: Article

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