Structure of the Ca 2+-dependent PP2A heterotrimer and insights into Cdc6 dephosphorylation

Cell Research

Volumn 23, Issue 7, 2013, Pages 931-946

  Wlodarchak, Nathan ^a   Guo, Feng ^a   Satyshur, Kenneth A ^a   Jiang, Li ^a   Jeffrey, Philip D ^b   Sun, Tingwan ^c   Stanevich, Vitali ^a   Mumby, Marc C ^c   Xing, Yongna ^a  

^a UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

^b PRINCETON UNIVERSITY   (United States)

^c UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

calcium binding motif; Cdc6; cell cycle; protein phosphatase 2A; regulatory subunit

Indexed keywords

CALCIUM; CELL CYCLE PROTEIN; HOLOENZYME; PHOSPHOPROTEIN PHOSPHATASE 2; PROTEIN SUBUNIT;

ANIMAL; CHEMISTRY; ENZYME SPECIFICITY; HUMAN; METABOLISM; PHOSPHORYLATION; PROTEIN SUBUNIT; X RAY CRYSTALLOGRAPHY; ARTICLE;

ANIMALS; CALCIUM; CELL CYCLE PROTEINS; CRYSTALLOGRAPHY, X-RAY; HOLOENZYMES; HUMANS; PHOSPHORYLATION; PROTEIN PHOSPHATASE 2; PROTEIN SUBUNITS; SUBSTRATE SPECIFICITY;

EID: 84879888186     PISSN: 10010602     EISSN: 17487838     Source Type: Journal    
DOI: 10.1038/cr.2013.77     Document Type: Article

References (43)

1. Hunter T. Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling. Cell 1995; 80:225-236.
2. Wurzenberger C, Gerlich DW. Phosphatases: providing safe passage through mitotic exit Nat Rev Mol Cell Biol 2011; 12:469-482.
3. Janssens V, Goris J. Protein phosphatase 2A: a highly regulat-ed family of serine/threonine phosphatases implicated in cell growth and signalling. Biochem J 2001; 353(Part 3):417-439. (Pubitemid 32158309)
4. Virshup DM. Protein phosphatase 2A: a panoply of enzymes. Curr Opin Cell Biol 2000; 12:180-185. (Pubitemid 30142564)
5. Shi Y. Serine/threonine phosphatases: mechanism through structure. Cell 2009; 139:468-484.
6. Ahn JH, Sung JY, McAvoy T, et al. The B″/PR72 subunit mediates Ca2+-dependent dephosphorylation of DARPP-32 by protein phosphatase 2A. Proc Natl Acad Sci USA 2007; 104:9876-9881. (Pubitemid 47175359)
7. Creyghton MP, Roel G, Eichhorn PJ, et al. PR130 is a modula-tor of the Wnt-signaling cascade that counters repression of the antagonist Naked cuticle. Proc Natl Acad Sci USA 2006; 103:5397-5402.
8. Creyghton MP, Roel G, Eichhorn PJ, et al. PR72, a novel regulator of Wnt signaling required for Naked cuticle function. Genes Dev 2005; 19:376-386. (Pubitemid 40189300)
9. Hall DD, Feekes JA, Arachchige Don AS, et al. Binding of protein phosphatase 2A to the L-type calcium channel Cav1.2 next to Ser1928, its main PKA site, is critical for Ser1928 de-phosphorylation. Biochemistry 2006; 45:3448-3459. (Pubitemid 43376364)
10. Davare MA, Horne MC, Hell JW. Protein phosphatase 2A is associated with class C L-type calcium channels (Cav1.2) and antagonizes channel phosphorylation by cAMP-dependent protein kinase. J Biol Chem 2000; 275:39710-39717. (Pubitemid 32058999)
11. Sablina AA, Hector M, Colpaert N, Hahn WC. Identifcation of PP2A complexes and pathways involved in cell transformation. Cancer Res 2010; 70:10474-10484.
12. Magenta A, Fasanaro P, Romani S, et al. Protein phosphatase 2A subunit PR70 interacts with pRb and mediates its dephos-phorylation. Mol Cell Biol 2008; 28:873-882.
13. Yan Z, Fedorov SA, Mumby MC, Williams RS. PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6 and modulates DNA replication in human cells. Mol Cell Biol 2000; 20:1021-1029. (Pubitemid 30044239)
14. Davis AJ, Yan Z, Martinez B, Mumby MC. Protein phospha-tase 2A is targeted to cell division control protein 6 by a calci-um-binding regulatory subunit J Biol Chem 2008; 283:16104-16114.
15. Bell SP, Dutta A. DNA replication in eukaryotic cells. Annu Rev Biochem 2002; 71:333-374. (Pubitemid 34800224)
16. Duncker BP, Chesnokov IN, McConkey BJ. The origin recog-nition complex protein family. Genome Biol 2009; 10:214.
17. Harper JW, Burton JL, Solomon MJ. The anaphase-promoting complex: it's not just for mitosis any more. Genes Dev 2002; 16:2179-2206. (Pubitemid 35013081)
18. Mailand N, Diffey JF. CDKs promote DNA replication origin licensing in human cells by protecting Cdc6 from APC/C-dependent proteolysis. Cell 2005; 122:915-926. (Pubitemid 41345208)
19. Ruediger R, Hentz M, Fait J, Mumby M, Walter G. Molecular model of the A subunit of protein phosphatase 2A: interaction with other subunits and tumor antigens. J Virol 1994; 68:123-129.
20. Ruediger R, Roeckel D, Fait J, et al. Identifcation of binding sites on the regulatory A subunit of protein phosphatase 2A for the catalytic C subunit and for tumor antigens of simian virus 40 and polyomavirus. Mol Cell Biol 1992; 12:4872-4882.
21. Xing Y Xu Y, Chen Y, et al. Structure of protein phosphatase 2A core enzyme bound to tumor-inducing toxins. Cell 2006; 127:341-353. (Pubitemid 44572378)
22. Xu Y Chen Y, Zhang P, Jeffrey PD, Shi Y Structure of a pro-tein phosphatase 2A holoenzyme: insights into B55-mediated Tau dephosphorylation. Mol Cell 2008; 31:873-885.
23. Cho US, Xu W. Crystal structure of a protein phosphatase 2A heterotrimeric holoenzyme. Nature 2007; 445:53-57. (Pubitemid 46067298)
24. Xu Y, Xing Y, Chen Y et al. Structure of the protein phospha-tase 2A holoenzyme. Cell 2006; 127:1239-1251.
25. Janssens V, Jordens J, Stevens I, et al. Identifcation and func-tional analysis of two Ca2+-binding EF-hand motifs in the B″/PR72 subunit of protein phosphatase 2A. J Biol Chem 2003; 278:10697-10706. (Pubitemid 36800340)
26. Adams SR, Tsien RY. Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins. Nat Protoc 2008; 3:1527-1534.
27. Pollok BA, Heim R. Using GFP in FRET-based applications. Trends Cell Biol 1999; 9:57-60. (Pubitemid 29151793)
28. Hoffmann C, Gaietta G, Bunemann M, et al. A FlAsH-based FRET approach to determine G protein-coupled receptor acti-vation in living cells. Nat Methods 2005; 2:171-176. (Pubitemid 41122123)
29. Ragusa MJ, Dancheck B, Critton DA, et al. Spinophilin directs protein phosphatase 1 specifcity by blocking substrate binding sites. Nat Struct Mol Biol 2010; 17:459-464.
30. Saraf A, Oberg EA, Strack S. Molecular determinants for PP2A substrate specifcity: charged residues mediate dephos-phorylation of tyrosine hydroxylase by the PP2A/B′ regulatory subunit Biochemistry 2010; 49:986-995.
31. Terrak M, Kerff F, Langsetmo K, Tao T, Dominguez R. Struc-tural basis of protein phosphatase 1 regulation. Nature 2004; 429:780-784. (Pubitemid 38833131)
32. O'Connell N, Nichols SR, Heroes E, et al. The molecular basis for substrate specifcity of the nuclear NIPP1:PP1 holoenzyme. Structure 2012; 20:1746-1756.
33. Stanevich V, Jiang L, Satyshur KA, et al. The structural basis for tight control of PP2A methylation and function by LCMT-1. Mol Cell 2011; 41:331-342.
34. Virshup DM, Shenolikar S. From promiscuity to precision: protein phosphatases get a makeover. Mol Cell 2009; 33:537-545.
35. Otwinowski Z, Minor, W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol 1997; 276:307-326. (Pubitemid 27085611)
36. Ness SR, de Graaff RA, Abrahams JP, Pannu NS. CRANK: new methods for automated macromolecular crystal structure solution. Structure 2004; 12:1753-1761. (Pubitemid 39298960)
37. Winn MD, Ballard CC, Cowtan KD, et al. Overview of the CCP4 suite and current developments. Acta Crystallogr D Biol Crystallogr 2011; 67(Part 4):235-242.
38. de Graaff RA, Hilge M, van der Plas JL, Abrahams JP. Matrix methods for solving protein substructures of chlorine and sul-fur from anomalous data. Acta Crystallogr D Biol Crystallogr 2001; 57(Part 12):1857-1862. (Pubitemid 33145603)
39. Pannu NS, Read RJ. The application of multivariate statistical techniques improves single-wavelength anomalous diffrac-tion phasing. Acta Crystallogr D Biol Crystallogr 2004; 60(Pt 1):22-27. (Pubitemid 41308819)
40. Cowtan K. The Buccaneer software for automated model building. 1. Tracing protein chains. Acta Crystallogr D Biol Crystallogr 2006; 62(Part 9):1002-1011. (Pubitemid 44337374)
41. Emsley P, Cowtan K. Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 2004; 60(Part 12 Part 1):2126-2132. (Pubitemid 41742764)
42. Winn MD, Murshudov GN, Papiz MZ. Macromolecular TLS refnement in REFMAC at moderate resolutions. Methods En-zymol 2003; 374:300-321. (Pubitemid 37531815)
43. Chao Y, Xing Y, Chen Y, et al. Structure and mechanism of the phosphotyrosyl phosphatase activator. Mol Cell 2006; 23:535-546. (Pubitemid 44205496)