PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6 and modulates DNA replication in human cells

Molecular and Cellular Biology

Volumn 20, Issue 3, 2000, Pages 1021-1029

  Yan, Zhen ^a   Fedorov, Sergei A ^a   Mumby, Marc C ^a   Williams, R Sanders ^a,b  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHOPROTEIN; PHOSPHOPROTEIN PHOSPHATASE 2A;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL CYCLE G1 PHASE; DEPHOSPHORYLATION; DNA LIBRARY; DNA REPLICATION; EUKARYOTE; HUMAN; HUMAN CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN INTERACTION; YEAST;

AMINO ACID SEQUENCE; ANIMALS; CELL CYCLE PROTEINS; CELL LINE; CLONING, MOLECULAR; DNA REPLICATION; FEMALE; GENE LIBRARY; HELA CELLS; HUMANS; MACROMOLECULAR SUBSTANCES; MICE; MOLECULAR SEQUENCE DATA; PHOSPHOPROTEIN PHOSPHATASE; PHYLOGENY; PLACENTA; PREGNANCY; PROTEIN STRUCTURE, QUATERNARY; RABBITS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SPODOPTERA; TRANSFECTION;

EID: 0033972224     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.20.3.1021-1029.2000     Document Type: Article

References (45)

1. Bell, S. P., J. Mitchell, J. Leber, R. Kobayashi, and B. Stillman. 1995. The multidomain structure of ORC1p reveals similarity to regulators of DNA replication and transcriptional silencing. Cell 83:563-568.
2. Brown, G. W., P. V. Jallepalli, B. J. Huneycutt, and T. J. Kelly. 1997. Interaction of the S phase regulator cdc18 with cyclin-dependent kinase in fission yeast. Proc. Natl. Acad. Sci. USA 94:6142-6147.
3. Cegielska, A., S. Shaffer, R. Derua, J. Goris, and D. M. Virshup. 1994. Different oligomeric forms of protein phosphatase 2A activate and inhibit simian virus 40 DNA replication. Mol. Cell. Biol. 14:4616-4623.
4. Coleman, T. R., P. B. Carpenter, and W. G. Dunphy. 1996. The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts. Cell 87:53-63.
5. Detweiler, C. S., and J. J. Li. 1998. Ectopic induction of Clb2 in early G1 phase is sufficient to block prereplicative complex formation in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 95:2384-2389.
6. Dutta, A., and S. P. Bell. 1997. Initiation of DNA replication in eukaryotic cells. Annu. Rev. Cell Dev. Biol. 13:293-332.
7. Fields, S., and O. Song. 1989. A novel genetic system to detect protein-protein interactions. Nature 340:245-246.
8. Gavin, K. A., M. Hidaka, and B. Stillman. 1995. Conserved initiator proteins in eukaryotes. Science 270:1667-1671.
9. Hartwell, L. 1976. Sequential function of gene products relative to DNA synthesis in the yeast cell cycle. J. Cell Biol. 104:803-817.
10. Hateboer, G., A. Wobst, B. O. Petersen, L. Le Cam, E. Vigo, C. Sardet, and K. Helin. 1998. Cell cycle-regulated expression of mammalian CDC6 is dependent on E2F. Mol. Cell. Biol. 18:6679-6697.
11. Hendrix, P., R. E. Mayer-Jackel, P. Cron, J. Goris, J. Hofsteenge, W. Merlevede, and B. A. Hemmings. 1993. Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing. J. Biol. Chem. 268:15267-15276.
12. Jallepalli, P. V., D. Tien, and T. J. Kelly. 1998. sud1(+) targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast. Proc. Natl. Acad. Sci. USA 95:8159-8164.
13. Jiang, W., N. J. Wells, and T. Hunter. 1999. Multistep regulation of DNA replication by cdk phosphorylation of HsCdc6. Proc. Natl. Acad. Sci. USA 96:6193-6198.
14. Kamibayashi, C., R. Estes, R. L. Lickteig, S. I. Yang, C. Craft, and M. C. Mumby. 1994. Comparison of heterotrimeric protein phosphatase 2A containing different B subunits. J. Biol. Chem. 269:20139-20148.
15. Kelly, T. J., P. Nurse, and S. L. Forsburg. 1993. Coupling DNA replication to the cell cycle. Cold Spring Harbor Symp. Quant. Biol. 58:637-644.
16. Leone, G., J. DeGregori, Z. Yan, L. Jakoi, S. Ishida, R. S. Williams, and J. R. Nevins. 1998. E2F3 activity is regulated during the cell cycle and is required for the induction of S phase. Genes Dev. 12:2120-2130.
17. Liang, C., and B. Stillman. 1997. Persistent initiation of DNA replication and chromatin-bound MCM proteins during the cell cycle in cdc6 mutants. Genes Dev. 11:3375-3386.
18. Liang, C., M. Weinreich, and B. Stillman. 1995. ORC and Cdc6p interact and determine the frequency of initiation of DNA replication in the genome. Cell 81:667-676.
19. Lin, F. C., and K. T. Arndt. 1995. The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosis. EMBO J. 14:2745-2759.
20. Lin, X. H., J. Walter, K. Scheidtmann, K. Ohst, J. Newport, and G. Walter. 1998. Protein phosphatase 2A is required for the initiation of chromosomal DNA replication. Proc. Natl. Acad. Sci. USA 95:14693-14698.
21. Lopez-Girona, A., O. Mondesert, J. Leatherwood, and P. Russell. 1998. Negative regulation of Cdc18 DNA replication protein by Cdc2. Mol. Biol. Cell 9:63-73.
22. Madine, M. A., C. Y. Khoo, A. D. Mills, and R. A. Laskey. 1995. MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells. Nature 375:421-424.
23. McCright, B., and D. M. Virshup. 1995. Identification of a new family of protein phosphatase 2A regulatory subunits. J. Biol. Chem. 270:26123-26128.
24. Milan, M., and S. M. Cohen. 1999. Regulation of LIM homeodomain activity in vivo: a tetramer of dLDB and apterous confers activity and capacity for regulation by dLMO. Mol. Cell 4:267-273.
25. Mumby, M. C., and G. Walter. 1993. Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth. Physiol. Rev. 73:673-699.
26. Muzi-Falconi, M., G. W. Brown, and T. J. Kelly. 1996. cdc18+ regulates initiation of DNA replication in Schizosaccharomyces pombe. Proc. Natl. Acad. Sci. USA 93:1566-1570.
27. Nasmyth, K. 1996. Viewpoints: putting the cell cycle in order. Science 274: 1643-1645.
28. cdc18 plays a major role controlling the initiation of DNA replication in fission yeast. Cell 83:397-405.
29. Petersen, B. O., J. Lukas, C. S. Sorensen, J. Bartek, and K. Helin. 1999. Phosphorylation of mammalian CDC6 by cyclin A/CDK2 regulates its sub-cellular localization. EMBO J. 18:396-410.
30. 1 is important for the onset of S phase and for preventing a "reductional" anaphase in the budding yeast Saccharomyces cerevisiae. EMBO J. 1141:3788-3799.
31. Rowles, A., and J. J. Blow. 1997. Chromatin proteins involved in the initiation of DNA replication. Curr. Opin. Genet. Dev. 7:152-157.
32. Saha, P., J. Chen, K. C. Thome, S. J. Lawlis, Z. H. Hou, M. Hendricks, J. D. Parvin, and A. Dutta. 1998. Human CDC6/Cdc18 associates with Orc1 and cyclin-cdk and is selectively eliminated from the nucleus at the onset of S phase. Mol. Cell. Biol. 18:2758-2767.
33. Scheidtmann, K. H., M. C. Mumby, K. Rundell, and G. Walter. 1991. Dephosphorylation of simian virus 40 large-T antigen and p53 protein by protein phosphatase 2A: inhibition by small-t antigen. Mol. Cell. Biol. 11:1996-2003.
34. Stillman, B. 1996. Cell cycle control of DNA replication. Science 274:1659-1664.
35. Stillman, B. 1994. Smart machines at the DNA replication fork. Cell 78:725-728.
36. Stoeber, K., A. D. Mills, Y. Kubota, T. Krude, P. Romanowski, K. Marheineke, R. A. Laskey, and G. H. Williams. 1998. Cdc6 protein causes premature entry into S phase in a mammalian cell-free system. EMBO J. 17:7219-7229.
37. Tehrani, M. A., M. C. Mumby, and C. Kamibayashi. 1996. Identification of a novel protein phosphatase 2A regulatory subunit highly expressed in muscle. J. Biol. Chem. 271:5164-5170.
38. Voorhoeve, P. M., E. M. Hijmans, and R. Bernards. 1999. Functional interaction between a novel protein phosphatase 2A regulatory subunit, PR59, and the retinoblastoma-related p107 protein. Oncogene 18:515-524.
39. Wera, S., and B. A. Hemmings. 1995. Serine/threonine protein phosphatases. Biochem. J. 311:17-29.
40. Williams, G. H., P. Romanowski, L. Morris, M. Madine, A. D. Mills, K. Stoeber, J. Marr, R. A. Laskey, and N. Coleman. 1998. Improved cervical smear assessment using antibodies against proteins that regulate DNA replication. Proc. Natl. Acad. Sci. USA 95:14932-14937.
41. Williams, R. S., R. V. Shohet, and B. Stillman. 1997. A human protein related to yeast Cdc6p. Proc. Natl. Acad. Sci. USA 94:142-147.
42. Van, Z., J. DeGregori, R. Shohet, G. Leone, B. Stillman, J. R. Nevins, and R. S. Williams. 1998. Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells. Proc. Natl. Acad. Sci. USA 95:3603-3608.
43. Yang, Q., R. Bassel-Duby, and R. S. Williams. 1997. Transient expression of a winged-helix protein, MNF-β, during myogenesis. Mol. Cell. Biol. 17:5236-5243.
44. Zolnierowicz, S., C. Csortos, J. Bondor, A. Verin, M. C. Mumby, and A. A. DePaoli-Roach. 1994. Diversity in the regulatory B-subunits of protein phosphatase 2A: identification of a novel isoform highly expressed in brain. Biochemistry 33:11858-11867.
45. Zou, L., and B. Stillman. 1998. Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280:593-596.