P-glycoprotein functions as an immunomodulator in healthy human primary nasal epithelial cells

International Forum of Allergy and Rhinology

Volumn 3, Issue 6, 2013, Pages 433-438

  Bleier, Benjamin S ^a   Nocera, Angela L ^b   Iqbal, Hufsa ^b   Hoang, John D ^b   Feldman, Rachel E ^a   Han, Xue ^b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b Boston University   (United States)

Author keywords

Cell culture; Cytokine secretion; Immunomodulator; Multidrug resistance; Nasal epithelium; P glycoprotein; PSC 833; Rhodamine 123; Sinusitis; Verapamil

Indexed keywords

GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; INTERLEUKIN 6; INTERLEUKIN 8; LIPOPOLYSACCHARIDE; MULTIDRUG RESISTANCE PROTEIN; RHODAMINE 123; THYMIC STROMAL LYMPHOPOIETIN; VALSPODAR; VERAPAMIL;

ADAPTIVE IMMUNITY; ARTICLE; CONTROLLED STUDY; CYTOKINE RELEASE; DOSE RESPONSE; DRUG EXPOSURE; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITHELIUM CELL; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IMMUNOMODULATION; INNATE IMMUNITY; NOSE MUCOSA; NOSE POLYP; PRIMARY NASAL EPITHELIAL CELL CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; QUANTITATIVE FLUORESCENT IMMUNOHISTOCHEMISTRY; SENSITIVITY ANALYSIS; UPREGULATION;

CYTOKINES; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITHELIAL CELLS; FLUORESCENT DYES; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; HUMANS; INTERLEUKIN-6; INTERLEUKIN-8; LIPOPOLYSACCHARIDES; NASAL CAVITY; P-GLYCOPROTEIN; RHODAMINE 123; VERAPAMIL;

EID: 84879717043     PISSN: 20426976     EISSN: 20426984     Source Type: Journal    
DOI: 10.1002/alr.21166     Document Type: Article

References (16)

1. Fernandez C, Buyse M, German-Fattal M, Gimenez F. Influence of the pro-inflammatory cytokines on P-glycoprotein expression and functionality. J Pharm Pharm Sci. 2004;7:359-371.
2. Golden PL, Pollack GM. Blood-brain barrier efflux transport. J Pharm Sci. 2003;92:1739-1753.
3. Cleves AE, Kelly RB. Rehearsing the ABCs. Protein translocation. Curr Biol. 1996;6:276-278.
4. Bleier BS. Regional expression of epithelial MDR1/P-glycoprotein in chronic rhinosinusitis with and without nasal polyposis. Int Forum Allergy Rhinol. 2012;2:122-125.
5. van der Deen M, Marks H, Willemse BW, Postma DS, et al. Diminished expression of multidrug resistance-associated protein 1 (MRP1) in bronchial epithelium of COPD patients. Virchows Arch. 2006;449:682-688.
6. Wioland MA, Fleury-Feith J, Corlieu P, et al. CFTR, MDR1, and MRP1 immunolocalization in normal human nasal respiratory mucosa. J Histochem Cytochem. 2000;48:1215-1222.
7. Kandimalla KK, Donovan MD. Localization and differential activity of P-glycoprotein in the bovine olfactory and nasal respiratory mucosae. Pharm Res. 2005;22:1121-1128.
8. Drach J, Gsur A, Hamilton G, et al. Involvement of P-glycoprotein in the transmembrane transport of interleukin-2 (IL-2), IL-4, and interferon-gamma in normal human T lymphocytes. Blood. 1996;88:1747-1754.
9. Kooij G, Backer R, Koning JJ, et al. P-glycoprotein acts as an immunomodulator during neuroinflammation. PLoS One. 2009;4:e8212.
10. Morjani H, Madoulet C. Immunosuppressors as multidrug resistance reversal agents. Methods Mol Biol. 2010;596:433-446.
11. Reh DD, Wang Y, Ramanathan M Jr, et al. Treatment-recalcitrant chronic rhinosinusitis with polyps is associated with altered epithelial cell expression of interleukin-33. Am J Rhinol Allergy. 2010;24:105-109.
12. Lane AP. The role of innate immunity in the pathogenesis of chronic rhinosinusitis. Curr Allergy Asthma Rep. 2009;9:205-212.
13. Ramanathan M Jr, Lee WK, Dubin MG, et al. Sinonasal epithelial cell expression of toll-like receptor 9 is decreased in chronic rhinosinusitis with polyps. Am J Rhinol. 2007;21:110-116.
14. Ramanathan M Jr, Spannhake EW, Lane AP. Chronic rhinosinusitis with nasal polyps is associated with decreased expression of mucosal interleukin 22 receptor. Laryngoscope. 2007;117:1839-1843.
15. Cho HJ, Choi MK, Lin H, et al. Expression and functional activity of P-glycoprotein in passaged primary human nasal epithelial cell monolayers cultured by the air-liquid interface method for nasal drug transport study. J Pharm Pharmacol. 2011;63:385-391.
16. Eder C. Mechanisms of interleukin-1beta release. Immunobiology. 2009;214:543-553.