Expression and functional activity of P-glycoprotein in passaged primary human nasal epithelial cell monolayers cultured by the air-liquid interface method for nasal drug transport study

Journal of Pharmacy and Pharmacology

Volumn 63, Issue 3, 2011, Pages 385-391

  Cho, Hyun Jong ^a   Choi, Min Koo ^b   Lin, Hongxia ^a   Kim, Jung Sun ^c   Chung, Suk Jae ^a   Shim, Chang Koo ^a   Kim, Dae Duk ^a  

^a Seoul National University   (South Korea)

^b Dankook University   (South Korea)

^c Dongseo University   (South Korea)

Author keywords

Human nasal epithelial cell monolayer; In vitro models; Nasal drug delivery; P glycoprotein

Indexed keywords

CYCLOSPORIN A; GLYCOPROTEIN P; MULTIDRUG RESISTANCE PROTEIN 1; RHODAMINE 123; SODIUM AZIDE; VERAPAMIL;

AIR LIQUID INTERFACE METHOD; ANALYTIC METHOD; ARTICLE; CELL TRANSPORT; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG DELIVERY SYSTEM; DRUG TRANSPORT; EPITHELIUM CELL; GENE EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IN VITRO STUDY; MONOLAYER CULTURE; NOSE MUCOSA; PROTEIN EXPRESSION; PROTEIN FUNCTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

BIOLOGICAL TRANSPORT; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DRUG ADMINISTRATION ROUTES; FLUORESCENT DYES; GENE EXPRESSION; GENE EXPRESSION REGULATION; HUMANS; MODELS, BIOLOGICAL; NASAL MUCOSA; P-GLYCOPROTEIN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHODAMINE 123; RNA, MESSENGER;

EID: 79952216010     PISSN: 00223573     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.2042-7158.2010.01221.x     Document Type: Article

References (31)

1. Shilling RA et al. New light on multidrug binding by an ATP-binding-cassette transporter. Trends Pharmacol Sci 2006; 27: 195-203.
2. Thiebaut F et al. Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues. Proc Natl Acad Sci USA 1987; 84: 7735-7738.
3. O'Connor R. The pharmacology of cancer resistance. Anticancer Res 2007; 27: 1267-1272. (Pubitemid 46813088)
4. Lechapt-Zalcman E et al. MDR1-Pgp 170 expression in human bronchus. Eur Respir J 1997; 10: 1837-1843. (Pubitemid 27368379)
5. Ehrhadt C et al. 16HBE14o- human bronchial epithelial cell layers express P-glycoprotein, lung resistance-related protein, and caveolin-1. Pharm Res 2003; 20: 545-551.
6. Florea BI et al. Evidence of p-glycoprotein mediated apical to basolateral transport of flunisolide in human broncho-tracheal epithelial cells (Calu-3). Br J Pharmacol 2001; 134: 1555-1563. (Pubitemid 33131907)
7. Brouillard F et al. Drug resistance induced by ouabain via the stimulation of MDR1 gene expression in human carcinomatous pulmonary cells. Cancer Res 2001; 61: 1693-1698. (Pubitemid 34292607)
8. Lin H et al. Air-liquid interface (ALI) culture of human bronchial epithelial cell monolayers as an in vitro model for airway drug transport studies. J Pharm Sci 2007; 96: 341-350. (Pubitemid 46363565)
9. Wioland MA et al. CFTR, MDR1, and MRP1 immunolocalization in normal human nasal respiratory mucosa. J Histochem Cytochem 2000; 48: 1215-1222.
10. Costantino HR et al. Intranasal delivery: physicochemical and therapeutic aspects. Int J Pharm 2007; 337: 1-24. (Pubitemid 46756063)
11. Ugwoke MI et al. Nasal mucoadhesive drug delivery: background, applications, trends and future perspectives. Adv Drug Deliv Rev 2005; 57: 1640-1665. (Pubitemid 41443473)
12. Khan S et al. Brain targeting studies on buspirone hydrochloride after intranasal administration of mucoadhesive formulation in rats. J Pharm Pharmacol 2009; 61: 669-675.
13. Jintapattanakit A et al. Nasal absorption and local tissue reaction of insulin nanocomplexes of trimethyl chitosan derivatives in rats. J Pharm Pharmacol 2010; 62: 583-591.
14. Merkle HP et al. In vitro cell models to study nasal mucosal permeability and metabolism. Adv Drug Deliv Rev 1998; 29: 51-79. (Pubitemid 28044086)
15. Agu RU et al. Specific aminopeptidases of excised human nasal epithelium and primary culture: a comparison of functional characteristics and gene transcripts expression. J Pharm Pharmacol 2009; 61: 599-606.
16. Yoo JW et al. Serially passaged human nasal epithelial cell monolayer for in vitro drug transport studies. Pharm Res 2003; 20: 1690-1696.
17. Lin H et al. Transport of anti-allergic drugs across the passage cultured human nasal epithelial cell monolayer. Eur J Pharm Sci 2005; 26: 203-210. (Pubitemid 41169803)
18. Lee MK et al. Air-liquid interface culture of serially passaged human nasal epithelial cell monolayer for in vitro drug transport studies. Drug Deliv 2005; 12: 305-311.
19. Huh Y et al. Preparation and evaluation of spray-dried hyaluronic acid microspheres for intranasal delivery of fexofenadine hydrochloride. Eur J Pharm Sci 2010; 40: 9-15.
20. Endter S et al. P-glycoprotein (MDR1) functional activity in human alveolar epithelial cell monolayers. Cell Tissue Res 2007; 328: 77-84. (Pubitemid 46411916)
21. Yumoto R et al. Transport of rhodamine123, a P-glycoprotein substrate, across rat intestine and Caco-2 cell monolayers in the presence of cytochrome P-450 3A-related compounds. J Pharmacol Exp Ther 1999; 289: 149-155. (Pubitemid 29156023)
22. Itagaki F et al. Effect of rabeprazole on MDR1-mediated transport of rhodamine123 in Caco-2 and Hvr100-6 cells. Biol Pharm Bull 2004; 27: 1694-1696. (Pubitemid 41701667)
23. Van der Sandt IC et al. Specificity of doxorubicin versus rhodamine-123 in assessing P-glycoprotein functionality in the LLC-PK1, LLC-PK1 : MDR1 and Caco-2 cell lines. Eur J Pharm Sci 2000; 11: 207-214.
24. Seeger MA, van Veen HW. Molecular basis of multidrug transport by ABC transporters. Biochim Biophys Acta 2009; 1794: 725-737.
25. Bates DA et al. Effects of thermal adaptation at 40°C on membrane viscosity and the sodium-potassium pump in Chinese hamster ovary cells. Cancer Res 1985; 45: 4895-4899. (Pubitemid 16236168)
26. Hamilton KO et al. P-glycoprotein efflux pump expression and activity in Calu-3 cells. J Pharm Sci 2001; 90: 647-658. (Pubitemid 32378386)
27. Troutman MD, Thakker DR. Rhodamine 123 requires carrier-mediated influx for its activity as a P-glycoprotein substrate in a Caco-2 cells. Pharm Res 2003; 20: 1192-1199. (Pubitemid 36951844)
28. Mallants R et al. An improved primary human nasal cell culture for the simultaneous determination of transepithelial transport and ciliary beat frequency. J Pharm Pharmacol 2009; 61: 883-890.
29. Graff CL, Pollack GM. P-Glycoprotein attenuates brain uptake of substrates after nasal instillation. Pharm Res 2003; 20: 1225-1230. (Pubitemid 36951847)
30. Graff CL, Pollack GM. Functional evidence for P-glycoprotein at the nose-brain barrier. Pharm Res 2005; 22: 86-93. (Pubitemid 40558150)
31. Shyeilla V et al. Intranasal delivery to the central nervous system: mechanisms and experimental considerations. J Pharm Sci 2010; 99: 1654-1673.