Generation of deletion and point mutations with one primer in a single cloning step

BioTechniques

Volumn 29, Issue 5, 2000, Pages 970-972

  Makarova, O ^a   Kamberov, E ^a   Margolis, B ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; DELETION MUTANT; DNA DENATURATION; GENE AMPLIFICATION; MOLECULAR CLONING; POINT MUTATION; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

EID: 0033729426     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00295bm08     Document Type: Article

References (9)

1. Ligand binding sites on guinea pig C3aR: Point and deletion mutations in the large extracellular loop and vicinity
2. Molecular cloning and characterization of Pals, proteins associated with mL in-7
3. Multiple site mutagenesis with high targeting efficiency in one cloning step
4. Site-directed mutagenesis in one day with >80% efficiency
5. Random mutagenesis by whole-plasmid PCR amplification
6. Site-specific mutagenesis of almost any plasmid using a PCR-based version of unique site elimination
7. Multiple mutant cDNAs from one reaction mixture using asymmetric primers in PCR
8. Two-Stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange(TM) site-directed mutagenesis
9. Adaptation of inverse PCR to generate an internal deletion