Rapid 16S rRNA Next-Generation Sequencing of Polymicrobial Clinical Samples for Diagnosis of Complex Bacterial Infections

PLoS ONE

Volumn 8, Issue 5, 2013, Pages

  Salipante, Stephen J ^a   Sengupta, Dhruba J ^a   Rosenthal, Christopher ^a   Costa, Gina ^b   Spangler, Jessica ^b   Sims, Elizabeth H ^a   Jacobs, Michael A ^a   Miller, Samuel I ^a   Hoogestraat, Daniel R ^a   Cookson, Brad T ^a   McCoy, Connor ^c   Matsen, Frederick A ^c   Shendure, Jay ^a   Lee, Clarence C ^b   Harkins, Timothy T ^b   Hoffman, Noah G ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

^b Life Technologies   (United States)

^c Fred Hutchinson Cancer Research Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

ARTICLE; BACTERIAL INFECTION; BURKHOLDERIA CEPACIA; CYSTIC FIBROSIS; DEEP SEQUENCING; GENETIC HETEROGENEITY; GENETIC VARIABILITY; HUMAN; METAGENOMICS; MICROFLORA; MYCOBACTERIUM TUBERCULOSIS; PHYLOGENY; PREVALENCE; PSEUDOMONAS AERUGINOSA; RNA ANALYSIS; RNA SEQUENCE; SEMICONDUCTOR; SEQUENCE ANALYSIS; SPECIES COMPOSITION; SPECIES DIFFERENTIATION; SPUTUM ANALYSIS; STREPTOCOCCUS PYOGENES; TAXONOMY;

BACTERIA; BACTERIAL INFECTIONS; BACTERIAL TYPING TECHNIQUES; CYSTIC FIBROSIS; DNA, BACTERIAL; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; METAGENOME; MICROBIOTA; PHYLOGENY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL, 16S; SPECIES SPECIFICITY; SPUTUM;

BACTERIA (MICROORGANISMS);

EID: 84878433385     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0065226     Document Type: Article

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