Protein covalent dimer formation induced by reversed-phase HPLC conditions

Journal of Pharmaceutical Sciences

Volumn 102, Issue 3, 2013, Pages 842-851

  Huang, Xian ^a,b   Barnard, James ^a,c   Spitznagel, Thomas M ^a   Krishnamurthy, Rajesh ^a,d  

^a GLAXOSMITHKLINE   (United States)

^b CMC Biologics   (United States)

^c UNIVERSITY OF COLORADO   (United States)

^d Zyngenia Inc   (United States)

Author keywords

Analytical biochemistry; Cysteine oxidation; Cysteine sulfenic acid; HPLC; Liquid chromatography; Oxidation; Protein aggregation (dimerization); Reversed phase

Indexed keywords

CYSTEINE; DIMER; DISULFIDE; FORMIC ACID; ORGANIC SOLVENT; SULFENIC ACID DERIVATIVE; TRIFLUOROACETIC ACID;

ARTICLE; CONTROLLED STUDY; DIMERIZATION; ELECTROSPRAY MASS SPECTROMETRY; OXIDATION; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECURSOR; PROTEIN POLYMERIZATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SIMULATION;

EID: 84878129463     PISSN: 00223549     EISSN: 15206017     Source Type: Journal    
DOI: 10.1002/jps.23431     Document Type: Article

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