Consumption of transgenic cows' milk containing human lactoferrin results in beneficial changes in the gastrointestinal tract and systemic health of young pigs

Transgenic Research

Volumn 22, Issue 3, 2013, Pages 571-578

  Cooper, Caitlin A ^a   Nelson, Kathryn M ^b   Maga, Elizabeth A ^a   Murray, James D ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b Pharming Group NV   (Netherlands)

Author keywords

Crypt; Intestine; Lactoferrin; Leukocytes; Milk; Transgenic cows

Indexed keywords

CYTOKINE; LACTOFERRIN;

ANIMAL; ARTICLE; CATTLE; CYTOLOGY; DRUG EFFECT; DUODENUM; ENTEROBACTERIACEAE; ESCHERICHIA COLI; FEMALE; GASTROINTESTINAL TRACT; GENETICALLY MODIFIED FOOD; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; ILEUM; INFLAMMATION; LEUKOCYTE; MALE; METABOLISM; MICROBIOLOGY; MILK; PHYSIOLOGY; SWINE; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CATTLE; CYTOKINES; DUODENUM; ENTEROBACTERIACEAE; ESCHERICHIA COLI; FEMALE; FOOD, GENETICALLY MODIFIED; GASTROINTESTINAL TRACT; HUMANS; ILEUM; INFLAMMATION; LACTOFERRIN; LEUKOCYTES; MALE; MILK; SWINE;

ANIMALIA; BOS; SUIDAE;

EID: 84877740988     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-012-9662-7     Document Type: Article

References (38)

1. Actor JK, Hwang SA, Kruzel ML (2009) Lactoferrin as a natural immune modulator. Curr Pharm Des 15: 1956-1973.
2. Azab B, Daoud J, Naeem FB, Nasr R, Ross J, Ghimire P, Siddiqui A, Azzi N, Rihana N, Abdallah M, Azzi N, Patel P, Kleiner M, El-Sayegh S (2012) Neutrophil-to-Lymphocyte Ratio as a Predictor of Worsening Renal Function in Diabetic Patients (3-Year Follow-Up Study). Ren Fail PMID 22452450.
3. Baker EN, Baker HM (2009) A structural framework for understanding the multifunctional character of lactoferrin. Biochimie 91: 3-10.
4. Berman HA, Weinstein L (1971) Antibiotics and nutrition. Am J Clin Nutr 24: 260-264.
5. Brundige DR, Maga EA, Klasing KC, Murray JD (2008) Lysozyme transgenic goats' milk influences gastrointestinal morphology in young pigs. J Nutr 138: 921-926.
6. Cheng JB, Wang JQ, Bu DP, Liu GL, Zhang CG, Wei HY, Zhou LY, Wang JZ (2008) Factors affecting the lactoferrin concentration in bovine milk. J Dairy Sci 91: 970-976.
7. Commare CE, Tappenden KA (2007) Development of the infant intestine: implications for nutrition support. Nutr Clin Pract 22: 159-173.
8. Cooper CA, Brundige DR, Reh WA, Maga EA, Murray JD (2011) Lysozyme transgenic goats' milk positively impacts intestinal cytokine expression and morphology. Transgenic Res 20: 1235-1243.
9. Guilloteau P, Zabielski R, Hammon HM, Metges CC (2010) Nutritional programming of gastrointestinal tract development. Is the pig a good model for man? Nutr Res Rev 23: 4-22.
10. Harada E, Itoh Y, Sitizyo K, Takeuchi T, Araki Y, Kitagawa H (1999) Characteristic transport of lactoferrin from the intestinal lumen into the bile via the blood in piglets. Comp Biochem Physiol A: Mol Integr Physiol 124: 321-327.
11. Hettinga K, van Valenberg H, de Vries S, Boeren S, van Hooijdonk T, van Arendonk J, Vervoort J (2011) The host defense proteome of human and bovine milk. PLoS ONE 6: e19433.
12. Humphrey BD, Huang N, Klasing KC (2002) Rice expressing lactoferrin and lysozyme has antibiotic-like properties when fed to chicks. J Nutr 132: 1214-1218.
13. Imtiaz F, Shafique K, Mirza SS, Ayoob Z, Vart P, Rao S (2012) Neutrophil lymphocyte ratio as a measure of systemic inflammation in prevalent chronic diseases in Asian population. Int Arch Med 5: 2.
14. Kanyshkova TG, Buneva VN, Nevinsky GA (2001) Lactoferrin and its biological functions. Biochem 66: 1-7.
15. Kim MH, Yang JY, Upadhaya SD, Lee HJ, Yun CH, Ha JK (2011) The stress of weaning influences serum levels of acute-phase proteins, iron-binding proteins, inflammatory cytokines, cortisol, and leukocyte subsets in Holstein calves. J Vet Sci 12: 151-157.
16. Koppelmann T, Pollak Y, Mogilner J, Bejar J, Coran AG, Sukhotnik I (2012) Reversal of severe methotrexate-induced intestinal damage using enteral n-3 fatty acids. Br J Nutr 28: 1-10.
17. Leitch EC, Wilcox MD (1999) Elucidation of the antistaphylococcal action of lactoferrin and lysozyme. J Med Micro 48: 867-871.
18. Li X, Swaggerty CL, Kogut MH, Chiang HI, Wang Y, Genovese KJ, He H, Zhou H (2010) Gene expression profiling of the local cecal response of genetic chicken lines that differ in their susceptibility to Campylobacter jejuni colonization. PLoS ONE 5: e11827.
19. Liao Y, Lopez V, Shafizadeh TB, Halsted CH, Lönnerdal B (2007) Cloning of a pig homologue of the human lactoferrin receptor: expression and localization during intestinal maturation in piglets. Comp Biochem Physiol A: Mol Integr Physiol 148: 584-590.
20. Liao Y, Jiang R, Lönnerdal B (2012) Biochemical and molecular impacts of lactoferrin on small intestinal growth and development during early life. Biochem Cell Biol 90: 476-484.
21. Liu D, Guo Y, Wang Z, Yuan J (2010) Exogenous lysozyme influences Clostridium perfringens colonization and intestinal barrier function in broiler chickens. Avian Pathol 39: 17-24.
22. Maga EA, Shoemaker CF, Rowe JD, Bondurant RH, Anderson GB, Murray JD (2006) Production and processing of milk from transgenic goats expressing human lysozyme in the mammary gland. J Dairy Sci 89: 518-524.
23. Maga EA, Desai PT, Weimer BC, Dao N, Kültz D, Murray JD (2012) Consumption of lysozyme-rich milk can alter microbial fecal populations. Appl Environ Microbiol 78: 6153-6160.
24. Mahmoud KZ, Edens FW (2012) Breeder age affects small intestine development of broiler chicks with immediate or delayed access to feed. Br Poult Sci 53: 32-41.
25. Montagne P, Cuillière ML, Molé C, Béné MC, Faure G (2001) Changes in lactoferrin and lysozyme levels in human milk during the first 12 weeks of lactation. Adv Exp Med Biol 501: 241-247.
26. Niekamp SR, Sutherland MA, Dahl GE, Salak-Johnson JL (2007) Immune responses of piglets to weaning stress: impacts of photoperiod. J Anim Sci 85: 93-100.
27. Nielsen SM, Hansen GH, Danielsen EM (2010) Lactoferrin targets T cells in the small intestine. J Gastroenterol 45: 1121-1128.
28. Pfaffl MW, Horgan GW, Dempfle L (2002) Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30: 36.
29. Salak-Johnson JL, McGlone JJ, Norman RL (1996) In vivo glucocorticoid effects on porcine natural killer cell activity and circulating leukocytes. J Anim Sci 74: 584-592.
30. Tang Z, Yin Y, Zhang Y, Huang R, Sun Z, Li T, Chu W, Kong X, Li L, Geng M, Tu Q (2009) Effects of dietary supplementation with an expressed fusion peptide bovine lactoferricin-lactoferrampin on performance, immune function and intestinal mucosal morphology in piglets weaned at age 21 d. Br J Nutr 101: 998-1005.
31. van Berkel PH, Welling MM, Geerts M, van Veen HA, Ravensbergen B, Salaheddine M, Pauwels EK, Pieper F, Nuijens JH, Nibbering PH (2002) Large scale production of recombinant human lactoferrin in the milk of transgenic cows. Nat Biotechnol 20: 484-487.
32. van der Peet-Schwering CM, Jansman AJ, Smidt H, Yoon I (2007) Effects of yeast culture on performance, gut integrity, and blood cell composition of weanling pigs. J Anim Sci 85: 3099-3109.
33. Wakabayashi H, Koji Yamauchi K, Takase M (2006) Lactoferrin research, technology and applications. Int Dairy J 16: 1241-1251.
34. Walker A (2010) Breast milk as the gold standard for protective nutrients. J Pediatr 156: S3-S7.
35. Wang Y, Shan T, Xu Z, Liu J, Feng J (2006) Effect of lactoferrin on the growth performance, intestinal morphology, and expression of PR-39 and protegrin-1 genes in weaned piglets. J Anim Sci 84: 2636-2641.
36. Wang R, Chen PJ, Chen WH (2011) Diet and exercise improve neutrophil to lymphocyte ratio in overweight adolescents. Int J Sports Med 32: 982-986.
37. Yen CC, Lin CY, Chong KY, Tsai TC, Shen CJ, Lin MF, Su CY, Chen HL, Chen CM (2009) Lactoferrin as a natural regimen for selective decontamination of the digestive tract: recombinant porcine lactoferrin expressed in the milk of transgenic mice protects neonates from pathogenic challenge in the gastrointestinal tract. J Infect Dis 199: 590-598.
38. Zhang P, Sawicki V, Lewis A, Hanson L, Nuijens JH, Neville MC (2001) Human lactoferrin in the milk of transgenic mice increases intestinal growth in ten-day-old suckling neonates. Adv Exp Med Biol 501: 107-113.