Molecular cloning and characterization of a thermostable esterase/lipase produced by a novel Anoxybacillus flavithermus strain

Journal of General and Applied Microbiology

Volumn 59, Issue 2, 2013, Pages 119-134

  Chiş, Laura ^a   Hriscu, Monica ^a   Bica, Adriana ^b   Toşa, Monica ^a   Nagy, Gergely ^c   Róna, Gergely ^c   Vértessy, Beata G ^c,d   Irimie, Florin Dan ^a  

^a BABE BOLYAI UNIVERSITY   (Romania)

^b INSTITUTE OF BIOLOGICAL RESEARCH   (Romania)

^c INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^d BUDAPEST UNIVERSITY OF TECHNOLOGY AND ECONOMICS   (Hungary)

Author keywords

Anoxybacillus flavithermus; Biochemical characterization; Enantioselectivity; Gene cloning and expression; Thermostable esterase lipase; Tridimensional structure

Indexed keywords

4 NITROPHENYLBUTYRIC ACID; ACETONITRILE; BUTYRIC ACID; DIMETHYL SULFOXIDE; DNA 16S; DODECYL SULFATE SODIUM; LONG CHAIN FATTY ACID; METHANOL; TRIACYLGLYCEROL LIPASE; TRIOLEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANOXYBACILLUS; ANOXYBACILLUS FLAVITHERMUS; ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BIOLOGICAL ACTIVITY; CONTROLLED STUDY; CYTOPLASM; DNA SEQUENCE; ENANTIOMER; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME METABOLISM; ENZYME STRUCTURE; HALF LIFE TIME; HYDROLYSIS; INCUBATION TEMPERATURE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PHENOTYPE; PHYSICAL TOLERANCE; POLYMERASE CHAIN REACTION; TEMPERATURE SENSITIVITY; THERMOSTABILITY;

AMINO ACID SEQUENCE; ANOXYBACILLUS; BACTERIAL TYPING TECHNIQUES; CLONING, MOLECULAR; CLUSTER ANALYSIS; DNA, BACTERIAL; DNA, RIBOSOMAL; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; ESTERASES; HOT SPRINGS; HYDROGEN-ION CONCENTRATION; LIPASE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PHYLOGENY; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; RNA, RIBOSOMAL, 16S; ROMANIA; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SOLVENTS; TEMPERATURE;

ANOXYBACILLUS; ANOXYBACILLUS FLAVITHERMUS; ESCHERICHIA COLI; GEOBACILLUS;

EID: 84877715173     PISSN: 00221260     EISSN: 13498037     Source Type: Journal    
DOI: 10.2323/jgam.59.119     Document Type: Article

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