Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus

Gene

Volumn 329, Issue 1-2, 2004, Pages 187-195

  Ewis, Hosam E ^a   Abdelal, Ahmed T ^b   Lu, Chung Dar ^a  

^a GEORGIA STATE UNIVERSITY   (United States)

^b NORTHEASTERN UNIVERSITY   (United States)

Author keywords

hydrolase; Bacillus; EDTA; Esterase; Ethylenediaminetetraacentate; Insertion sequence; IS; IS element; Kinetics; nt.; PAGE; PCR; Polyacrylamide gel electrophoresis; Polymerase chain reaction; Polyvinylidene difluoride; PVDF; SDS; Sodium dodecylsulphate

Indexed keywords

AMINO ACID; CARBOXYLESTERASE; ESTERASE; HEXANOIC ACID DERIVATIVE; POLYPEPTIDE; TRIACYLGLYCEROL LIPASE;

ARTICLE; BACTERIAL GENETICS; BACTERIAL STRAIN; CALCULATION; ENERGY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE LIBRARY; GENE LOCUS; GENETIC CODE; GENETIC SCREENING; GENOME; GEOBACILLUS STEAROTHERMOPHILUS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; SEQUENCE ANALYSIS; STATISTICAL SIGNIFICANCE; TEMPERATURE; THERMOSTABILITY; TIME;

BACILLUS STEAROTHERMOPHILUS; CARBOXYLIC ESTER HYDROLASES; DITHIOTHREITOL; DNA, BACTERIAL; ENZYME ACTIVATION; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; ISOENZYMES; KINETICS; MERCAPTOETHANOL; MOLECULAR SEQUENCE DATA; P-CHLOROMERCURIBENZOIC ACID; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY; TEMPERATURE;

ESCHERICHIA COLI; GEOBACILLUS; GEOBACILLUS STEAROTHERMOPHILUS; INSERTION SEQUENCES; UNIDENTIFIED INSERTION SEQUENCE;

EID: 1642411072     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.gene.2003.12.029     Document Type: Article

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