Linearization of the Bradford protein assay

Journal of Visualized Experiments

Volumn , Issue 38, 2010, Pages

  Ernst, Orna ^a   Zor, Tsaffrir ^a  

^a TEL AVIV UNIVERSITY   (Israel)

Author keywords

Bradford; Cellular biology; Coomassie brilliant blue; Issue 38; Protein assay; Protein quantification

Indexed keywords

EID: 80355148723     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1918     Document Type: Article

References (6)

1. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248-54 (1976).
2. Chial, H. J., Thompson, H. B. & Splittgerber, A. G. A spectral study of the charge forms of Coomassie blue G. Anal Biochem 209, 258-66 (1993).
3. Chial, H. J. & Splittgerber, A. G. A comparison of the binding of Coomassie brilliant blue to proteins at low and neutral pH. Anal Biochem 213, 362-9 (1993).
4. Compton, S. J. & Jones, C. G. Mechanism of dye response and interference in the Bradford protein assay. Anal Biochem 151, 369-74 (1985).
5. Congdon, R. W., Muth, G. W. & Splittgerber, A. G. The binding interaction of Coomassie blue with proteins. Anal Biochem 213, 407-13 (1993).
6. Zor, T. & Selinger, Z. Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies. Anal Biochem 236, 302-8 (1996).