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Volumn 10, Issue 1, 1999, Pages 17-20

Mass spectrometry detection and reduction of disulfide adducts between reducing agents and recombinant proteins with highly reactive cysteines

Author keywords

Disulfide adduct; Matrix assisted laser desorption and ionization mass spectrometry; Recombinant protein; Reducing agent; Tris(2 carboxyethyl)phosphine hydrochloride (TCEP HCL)

Indexed keywords


EID: 0003163442     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article
Times cited : (22)

References (6)
  • 1
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    • Physical properties of a single-motif erythrocyte spectrin peptide: A highly stable independently folding unit
    • DeSilva TM, Harper SL, Kotula L, et al. Physical properties of a single-motif erythrocyte spectrin peptide: a highly stable independently folding unit. Biochemistry 1997;36:3991-3997.
    • (1997) Biochemistry , vol.36 , pp. 3991-3997
    • Desilva, T.M.1    Harper, S.L.2    Kotula, L.3
  • 2
    • 57349153440 scopus 로고    scopus 로고
    • Expression and purification of GST fusion proteins
    • In Coligan JE, Dunn BM, Ploegh HL, Speicher DW, Wingfield PT (eds), New York: John Wiley & Sons
    • Harper S, Speicher DW. Expression and purification of GST fusion proteins. In Coligan JE, Dunn BM, Ploegh HL, Speicher DW, Wingfield PT (eds): Current Protocols in Protein Structure, vol 1. New York: John Wiley & Sons, 1997:6.6.1-6.6.21.
    • (1997) Current Protocols In Protein Structure , vol.1
    • Harper, S.1    Speicher, D.W.2
  • 3
    • 0028330535 scopus 로고
    • Isolation and functional characterisation of the A32 melanoma-associated antigen
    • Shih I-M, Elder DE, Speicher D, Johnson JP, Herlyn M. Isolation and functional characterisation of the A32 melanoma-associated antigen. Cancer Res 1994;54: 2514-2520.
    • (1994) Cancer Res , vol.54 , pp. 2514-2520
    • Shih, I.-M.1    Elder, D.E.2    Speicher, D.3    Johnson, J.P.4    Herlyn, M.5
  • 4
    • 0030800780 scopus 로고    scopus 로고
    • Melanoma cell-cell interactions are mediated through heterophilic Mel-CAM/ligand adhesion
    • Shih I-M, Speicher D, Hsu M-Y, Levine E, Herlyn M. Melanoma cell-cell interactions are mediated through heterophilic Mel-CAM/ligand adhesion. Cancer Res 1997;57:3835-3840.
    • (1997) Cancer Res , vol.57 , pp. 3835-3840
    • Shih, I.-M.1    Speicher, D.2    Hsu, M.-Y.3    Levine, E.4    Herlyn, M.5
  • 5
    • 0030051007 scopus 로고    scopus 로고
    • Direct isolation of proteins from sodium dodecyl sulfate-polyacrylamide gel electrophoresis and analysis by electrospray-ionization mass spectrometry
    • Schumacher M, Glocker MO, Wunderlin M, Przybylski M. Direct isolation of proteins from sodium dodecyl sulfate-polyacrylamide gel electrophoresis and analysis by electrospray-ionization mass spectrometry. Electrophoresis 1996;17:848-854.
    • (1996) Electrophoresis , vol.17 , pp. 848-854
    • Schumacher, M.1    Glocker, M.O.2    Wunderlin, M.3    Przybylski, M.4
  • 6
    • 0028360823 scopus 로고
    • A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothreitol
    • Han JC, Han GY. A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothreitol. Anal Biochem 1994;22:5-10.
    • (1994) Anal Biochem , vol.22 , pp. 5-10
    • Han, J.C.1    Han, G.Y.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.