Partial Purification and Characterization of a Bacteriocin DT24 Produced by Probiotic Vaginal Lactobacillus brevis DT24 and Determination of its Anti-Uropathogenic Escherichia coli Potential

Probiotics and Antimicrobial Proteins

Volumn 5, Issue 2, 2013, Pages 142-151

  Trivedi, Disha ^a   Jena, Prasant Kumar ^a   Patel, Jignesh Kumar ^a   Seshadri, Sriram ^a  

^a NIRMA UNIVERSITY   (India)

Author keywords

Bacteriocin; Characterization; Lactobacillus brevis; Mode of action; Purification; Uropathogenic Escherichia coli

Indexed keywords

AMMONIUM SULFATE; AMYLASE; BACTERIOCIN; BACTERIOCIN DT24; CATALASE; PEPSIN A; POLYACRYLAMIDE GEL; POLYPEPTIDE ANTIBIOTIC AGENT; PROBIOTIC AGENT; TRIACYLGLYCEROL LIPASE; TRYPSIN; UNCLASSIFIED DRUG;

ANTIBACTERIAL ACTIVITY; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; BACTERIOSTASIS; CONTROLLED STUDY; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; FEMALE; GEL FILTRATION CHROMATOGRAPHY; HEAT TOLERANCE; HUMAN; INFECTION CONTROL; LACTOBACILLUS BREVIS; LACTOBACILLUS BREVIS DT24; NONHUMAN; NORMAL HUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STAPHYLOCOCCUS AUREUS; URINARY TRACT INFECTION; UROPATHOGENIC ESCHERICHIA COLI; VAGINA FLORA;

ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ESCHERICHIA COLI; LACTOBACILLUS BREVIS; STAPHYLOCOCCUS AUREUS;

EID: 84877629322     PISSN: 18671306     EISSN: 18671314     Source Type: Journal    
DOI: 10.1007/s12602-013-9132-4     Document Type: Article

References (57)

1. Aasen IM, Markussen S, Møretrø T, Katla T, Axelsson L, Naterstad K (2003) Interactions of the bacteriocins sakacin P and nisin with food constituents. Int J Food Microbiol 87: 35-43.
2. Akram M, Shahid M, Khan AU (2007) Etiology and antibiotic resistance patterns of community-acquired urinary tract infections in J N M C Hospital Aligarh, India. Ann Clin Microbiol Antimicrobial 6(4). doi: 10. 1180/1476-0711-6-4.
3. Albano H, Todorov SD, van Reenen CA, Hogg T, Dicks LMT, Teixeira P (2007) Characterization of a bacteriocin produced by Pediococcus acidilactici isolated from "Alheira", afermented sausage traditionally produced in Portugal. Int J Food Microbiol 116: 239-247.
4. Aroutcheva A, Simoes JA, Faro S (2001) Antimicrobial protein produced by vaginal Lactobacillus acidophilus that inhibits Gardnerella vaginalis. Infect Dis Obstet Gynecol 9: 33-39.
5. Audisio MC, Oliver G, Apella MC (2001) Effect of different complex carbon sources on growth and bacteriocin synthesis of Enterococcus faecium. Int J Food Microbiol 63: 235-241.
6. Boris S, Suarez JE, Barbes C (1997) Characterization of the aggregation promoting factor from Lactobacillus gasseri, a vaginal isolate. J Appl Microbiol 83: s413-s420.
7. Casey PG, Casey GD, Gardiner GE, Tangney M, Stanton C, Ross RP et al (2004) Isolation and characterization of anti-Salmonella lactic acid bacteria from the porcine gastrointestinal tract. Lett Appl Microbiol 39: 431-438.
8. Collado MC, Meriluoto J, Salminen S (2007) Measurement of aggregation properties between probiotics and pathogens: in vitro evaluation of different methods. J Microbiol Methods 71(1): 71-74.
9. Colloca ME, Ahumada MC, López ME, Macías MEN (2000) Surface properties of lactobacilli isolated from healthy subjects. Oral Dis 6(4): 227-233.
10. Corsetti A, Settanni L, van Sinderen D (2004) Characterization of bacteriocin-like inhibitory substances (BLIS) from Sourdough lactic acid bacteria and evaluation of their in vitro and in situ activity. J Appl Microbiol 96: 521-534.
11. Cotter PD, Hill C, Ross RP (2005) Bacterial lantibiotics: strategies to improve therapeutic potential. Curr Protein Pept Sci 6: 61-75.
12. Cotter PD, Hill C, Ross RP (2005) Bacteriocin: developing innate immunity for food. Nat Rev Microbiol 3: 777-788.
13. Czaja CA, Scholes D, Hooton TM, Stamm WE (2007) Population based epidemiologic analysis of acute pyelonephritis. Clin Infect Dis 45: 273-280.
14. De Vuyst L, Vandamme EJ (1994) Bacteriocins of lactic acid bacteria: Microbiology, genetics and applications. In: de Vuyst L, Vandamme EJ (eds) Lactic acid bacteria and bacteriocins: their practical importance. Blackie Academic and Professional, London, pp 1-11.
15. Del Re B, Sgorbati B, Miglioli M, Palenzona D (2000) Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum. Lett Appl Microbiol 31: 438-442.
16. Dunne C, O'Mahony L, Murphy L, Thornton G, Morrissey D et al (2001) In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am J Clin Nutr 73: 386S-392S.
17. Dethlefsen L, Huse S, Sogin ML, Relman DA (2008) The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing. PLoS Biol 6(11): 2382-2400.
18. Ekmicki H, Belma A, Sahlan O (2009) Characterization of vaginal Lactobacilli coaggregation ability with Escherichia coli. Microbiol Immunol 53: 59-65.
19. Eschenbach DA, Davick PR, Williams BL, Klebanoff SJ, Young-Smith K, Critchlow CM et al (1989) Prevalence of hydrogen peroxide-producing Lactobacillus species in normal women and women with bacterial vaginosis. J Clin Microbiol 27: 251-256.
20. Foxman B, Ki M, Brown P (2007) Antibiotic resistance and pyelonephritis. Clin Infect Dis 45: 281-283.
21. Gänzle MG, Weber S, Hammes WP (1999) Effect of ecological factors on the inhibitory spectrum and activity of bacteriocins. Int J Food Microbiol 46: 207-217.
22. Garneau S, Martin NI, Vederas JC (2002) Two-peptide bacteriocins produced by lactic acid bacteria. Biochimie 84: 577-592.
23. Giri SS, Sukumaran V, Sen SS, Vinumoni J, Nazeema BB, Jena PK (2011) Antagonistic activity of cellular components of potential probiotic bacteria, isolated from the gut of Labeo rohita, against Aeromonas hydrophila. Probiotics Antimicrob Prot 3: 214-222.
24. Gong HS, Meng XC, Wang H (2010) Plantaricin MG active against Gram-negative bacteria produced by Lactobacillus plantarum KLDS1. 0391 isolated from "Jiaoke", a traditional fermented cream from China. Food Control 21: 89-96.
25. Guillilan SE (1979) Beneficial interrelationships between certain microorganisms and humans: candidate microorganisms for use as dietary adjuncts. J Food Prot 42: 164-167.
26. Gupta K, Hooton TM, Wobbe CL, Stamm WE (1999) The prevalence of antimicrobial resistance among uropathogens causing acute uncomplicated cystitis in young women. Int J Antimicrob Agents 11: 305-308.
27. Hernandez D, Cardell E, Zarate V (2005) Antimicrobial activity of lactic acid bacteria isolated from Tenerife cheese: initial characterization of plantaricin TF711, a bacteriocin-like substance produced by Lactobacillus plantarum TF711. J Appl Microbiol 99: 77-84.
28. Heu S, Oh JH, Kang YS, Ryu S, Cho SK, Cho YS et al (2001) Cloning, expression, and purification of glycinecin A, a bacteriocin produced by Xanthomonas campestris pv. glycines 8ra. App Environ Microbiol 67: 4105-4110.
29. Jena PK, Trivedi D, Chaudhary H, Sahoo TK et al (2013) Bacteriocin PJ4 active against enteric pathogen produced by Lactobacillus helveticus PJ4 isolated from gut microflora of Wistar Rat (Rattus norvegicus): partial purification and characterization of bacteriocin. Appl Biochem Biotechnol. doi: 10. 1007/s12010-012-0044-7.
30. Johnson JR, Stamm WE (1989) Urinary tract infections in women: diagnosis and treatment. Ann Intern Med 111: 906-917.
31. Jung DS, Bodyfelt FW, Daeschel MA (1992) Influence of fat and emulsifiers on the efficacy of nisin in inhibiting Listeria monocytogenes in fluid milk. J Dairy Science 75: 387-393.
32. Kaewsrichan J, Peeyananjarassri K, Kongprasertkit J (2006) Selection and identification of anaerobic lactobacilli producing inhibitory compounds against vaginal pathogens. FEMS Immunol Med Microbiol 48: 75-83.
33. Kim PI, Jung MY, Chang YH, Kim S, Kim SJ et al (2007) Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract. Appl Microbiol Biotechnol 74: 1103-1111.
34. Klaenhammer TR (1993) Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev 12: 39-86.
35. Kumar M, Tiwari SK, Srivastava S (2010) Purification and characterization of enterocin LR/6, a new bacteriocin from Enterococcus faecium LR/6. Appl Biochem Biotech 160: 40-49.
36. Kumar H, Rangrez A, Dayananda K, Atre A, Patole M et al (2011) Lactobacillus plantarum (VR1) isolated from an Ayurvedic medicine (Kutajarista) ameliorates in vitro cellular damage caused by Aeromonas veronii. BMC Microbiol 11: 152.
37. Macias MEN, Romero NC, Apella MC, Gonzalez SN, Oliver G (1993) Prevention of infections produced by E. coli and L. monocytogenes feeding milk fermented with lactobacilli. J Food Prot 56: 401-405.
38. Murray BE, Alvarado T, Kim KH, Vorachit M, Jayanetra P (1985) Increasing resistance to trimethoprim-sulfamethoxazole among isolates of Escherichia coli in developing countries. J Infect Dis 152: 1107-1113.
39. Nueno-Palop C, Narbad A (2011) Probiotic assessment of Enterococcus faecalis CP58 isolated from human gut. Int J Food Microbiol 145: 390-394.
40. O'Hanlon DE, Lanier BR, Moench TR, Cone RA (2010) Cervicovaginal fluid and semen block the microbicidal activity of hydrogen peroxide produced by vaginal lactobacilli. BMC Infect Dis 10(120): 1-8.
41. Pelletier C, Bouley C, Cayuela C, Boutier S, Bourlioux P, Bellon-Fontaine MN (1997) Cell surface characteristics of L. casei ss casei, L. paracasei ss paracasei and L. rhamnosus strains. Appl Environ Microbiol 63: 1725-1731.
42. Rattanachaikunsopon P, Phumkhachorn P (2006) Isolation and preliminary characterization of bacteriocin produced by Lactobacillus plantarum N014 isolated from nham, traditional Thai fermented pork. J Food Prot 69(8): 1937-1943.
43. Reid G (2001) Probiotic agents to protect urogenital tract against infection. Am J Clin Nutr 73(2): 437-443.
44. Ronald A (2003) The etiology of urinary tract infection: traditional and emerging pathogens. Am J Med 113(1): 14-19.
45. Schägger H, VonJagow G (1987) Tricine-Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis for the Separation of Proteins in the Range from 1 to 100 kDa. Anal Biochem 166: 368-379.
46. Servin AL (2004) Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens. FEMS Microbiol Rev 28: 405-440.
47. Sobel JD, Chaim W (1996) Vaginal microbiology of women with acute recurrent vulvovaginal candidiasis. J Clin Microbiol 34: 2497-2499.
48. Stergiou VA, Thomas LV, Adams MR (2006) Interactions of nisin with glutathione in a model protein system and meat. J Food Protect 69: 951-956.
49. Succi M, Tremonte P, Reale A, Sorrentino E, Grazia L, Pacifico S, Coppola R (2005) Bile salt and acid tolerance of Lactobacillus rhamnosus strains isolated from Parmigiano Reggiano cheese. FEMS Microbiol Lett 244(1): 129-137.
50. Sung C, Kim BG, Kim S, Joo HS, Kim PI (2010) Probiotic potential of Staphylococcus hominis MBBL 2-9 as anti-Staphylococcus aureus agent isolated from the vaginal microbiota of a healthy woman. J Appl Microbiol 108: 908-916.
51. Todorov S, Onno B, Sorokin O, Chobert JM, Ivanova I, Dousset X (1999) Detection and characterization of a novel antibacterial substance produced by Lactobacillus plantarum ST31 isolatedfrom sourdough. Int J Food Microbiol 48: 167-177.
52. Todorov SD, Dicks LMT (2004) Partial characterization of bacteriocins produced by four lactic acid bacteria isolated from regional South African barley beer. Ann Microbiol 54(4): 403-413.
53. Todorov SD, Dicks LMT (2005) Characterization of bacteriocins produced by lactic acid bacteria isolated from spoiled black olives. J Basic Microb 45: 312-322.
54. Todorov SD, Danova ST, VanReenen CA, Meincken M, Dinkova G et al (2006) Characterization of bacteriocin HV219, produced by Lactococcus lactis subsp. Lactis HV219isolated from human vaginal secretions. J Basic Microbiol 46: 226-238.
55. Todorov SD, Wachsman M, Tomé E, Dousset X, Destro MT, Dicks LMT et al (2010) Characterization of an antiviral pediocin-like bacteriocin produced by Enterococcusfaecium. Food Microbiol 27: 869-879.
56. Todorov SD, Furtado DN, Saad SMI, de Melo G, Franco BD (2011) Bacteriocin production and resistance to drugs are advantageous features for Lactobacillus acidophilus La-14, a potential probiotic strain. New Microbiol 34: 357-370.
57. Todorov SD, Prevost H, Lebosis M, Dousset X, LeBlanc JG, de Melo G et al (2011) Bacteriocinogenic Lactobacillus plantarum ST16 Pa isolated from papaya (Carica papaya)-from isolation to application: characterization of a bacteriocin. Food Res Int 44: 1351-1363.