An infectious HHV-6B isolate from a healthy adult with chromosomally integrated virus and a reporter based relative viral titer assay

Virus Research

Volumn 173, Issue 2, 2013, Pages 280-285

  Goel, Pallavi ^a   Tailor, Prafullakumar ^a,c   Chande, Ajit G ^a   Basu, Atanu ^b   Mukhopadhyaya, Robin ^a  

^a CANCER RESEARCH INSTITUTE   (India)

^b NATIONAL INSTITUTE OF VIROLOGY   (India)

^c NATIONAL INSTITUTE OF IMMUNOLOGY   (India)

Author keywords

CIHHV 6; HHV 6 titer assay; HHV 6B isolate; Indicator cell line; Non telomeric integration; Productive infection

Indexed keywords

GENOMIC DNA; LUCIFERASE;

ADULT; ARTICLE; ASSAY; B LYMPHOCYTE; CELL LINE; CHROMOSOME 12; CULTURE MEDIUM; DILUTION; DNA TEMPLATE; EPSTEIN BARR VIRUS; GRANULOCYTE; HUMAN; HUMAN CELL; HUMAN HERPESVIRUS 6; NONHUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; SOUTHERN BLOTTING; SUPERNATANT; T LYMPHOCYTE; VIRAL TITER ASSAY; VIRUS GENOME;

ADULT; ASYMPTOMATIC DISEASES; CHROMOSOMES; DNA, VIRAL; GRANULOCYTES; HERPESVIRUS 6, HUMAN; HUMANS; LEUKOCYTES, MONONUCLEAR; MALE; MOLECULAR SEQUENCE DATA; PROVIRUSES; ROSEOLOVIRUS INFECTIONS; SEQUENCE ANALYSIS, DNA; VIRAL LOAD;

HUMAN HERPESVIRUS 4; HUMAN HERPESVIRUS 6; HUMAN HERPESVIRUS 6B; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 84876861082     PISSN: 01681702     EISSN: 18727492     Source Type: Journal    
DOI: 10.1016/j.virusres.2013.02.006     Document Type: Article

References (35)

1. Arbuckle J.H., Medveczky M.M., Luka J., Hadley S.H., Luegmayr A., Ablashi D., Lund T.C., Tolar J., De Meirleir K., Montoya J.G., Komaroff A.L., Ambros P.F., Medveczky P.G. The latent human herpesvirus-6A genome specifically integrates in telomeres of human chromosomes in vivo and in vitro. Proceedings of the National Academy of Sciences of the United States of America 2010, 107(12):5563-5568.
2. Asada H., Yalcin S., Balachandra K., Higashi K., Yamanishi K. Establishment of titration system for human herpesvirus 6 and evaluation of neutralizing antibody response to the virus. Journal of Clinical Microbiology 1989, 27(10):2204-2207.
3. Bandobashi K., Daibata M., Kamioka M., Tanaka Y., Kubonishi I., Taguchi H., Ohtsuki Y., Miyoshi I. Human herpesvirus 6 (HHV-6)-positive Burkitt's lymphoma: establishment of a novel cell line infected with HHV-6. Blood 1997, 90(3):1200-1207.
4. Chande A.G., Baba M., Mukhopadhyaya R. Short communication: a single step assay for rapid evaluation of inhibitors targeting HIV type 1 Tat-mediated long terminal repeat transactivation. AIDS Research and Human Retroviruses 2012, 28(8):902-906.
5. Clark D.A., Griffiths P.D. Human herpesvirus 6: relevance of infection in the immunocompromised host. British Journal of Haematology 2003, 120(3):384-395.
6. Daibata M., Taguchi T., Sawada T., Taguchi H., Miyoshi I. Chromosomal transmission of human herpesvirus 6 DNA in acute lymphoblastic leukaemia. Lancet 1998, 352(9127):543-544.
7. De Bolle L., Naesens L., De Clercq E. Update on human herpesvirus 6 biology, clinical features, and therapy. Clinical Microbiology Reviews 2005, 18(1):217-245.
8. Decker L.L., Klaman L.D., Thorley-Lawson D.A. Detection of the latent form of Epstein-Barr virus DNA in the peripheral blood of healthy individuals. Journal of Virology 1996, 70(5):3286-3289.
9. Deng H., Dewhurst S. Functional identification and analysis of cis-acting sequences which mediate genome cleavage and packaging in human herpesvirus 6. Journal of Virology 1998, 72(1):320-329.
10. Di Luca D., Dolcetti R., Mirandola P., De Re V., Secchiero P., Carbone A., Boiocchi M., Cassai E. Human herpesvirus 6: a survey of presence and variant distribution in normal peripheral lymphocytes and lymphoproliferative disorders. Journal of Infectious Diseases 1994, 170(1):211-215.
11. Dockrell D.H. Human herpesvirus 6: molecular biology and clinical features. Journal of Medical Microbiology 2003, 52(Pt 1):5-18.
12. Dominguez G., Dambaugh T.R., Stamey F.R., Dewhurst S., Inoue N., Pellett P.E. Human herpesvirus 6B genome sequence: coding content and comparison with human herpesvirus 6A. Journal of Virology 1999, 73(10):8040-8052.
13. Fotheringham J., Donati D., Akhyani N., Fogdell-Hahn A., Vortmeyer A., Heiss J.D., Williams E., Weinstein S., Bruce D.A., Gaillard W.D., Sato S., Theodore W.H., Jacobson S. Association of human herpesvirus-6B with mesial temporal lobe epilepsy. PLoS Medicine 2007, 4(5):e180.
14. Gilbert C., Boivin G. New reporter cell line to evaluate the sequential emergence of multiple human cytomegalovirus mutations during in vitro drug exposure. Antimicrobial Agents and Chemotherapy 2005, 49(12):4860-4866.
15. Inoue N., Winter J., Lal R.B., Offermann M.K., Koyano S. Characterization of entry mechanisms of human herpesvirus 8 by using an Rta-dependent reporter cell line. Journal of Virology 2003, 77(14):8147-8152.
16. Isegawa Y., Mukai T., Nakano K., Kagawa M., Chen J., Mori Y., Sunagawa T., Kawanishi K., Sashihara J., Hata A., Zou P., Kosuge H., Yamanishi K. Comparison of the complete DNA sequences of human herpesvirus 6 variants A and B. Journal of Virology 1999, 73(10):8053-8063.
17. Joshi P.J., Merchant R.H., Pokharankar S.L., Damania K.S., Gilada I.S., Mukhopadhyaya R. Perinatally cotransmitted human herpesvirus 6 is activated in children born with human immunodeficiency virus infection. Journal of Human Virology 2000, 3(6):317-323.
18. Leong H.N., Tuke P.W., Tedder R.S., Khanom A.B., Eglin R.P., Atkinson C.E., Ward K.N., Griffiths P.D., Clark D.A. The prevalence of chromosomally integrated human herpesvirus 6 genomes in the blood of UK blood donors. Journal of Medical Virology 2007, 79(1):45-51.
19. Luka J., Kallin B., Klein G. Induction of the Epstein-Barr virus (EBV) cycle in latently infected cells by n-butyrate. Virology 1979, 94(1):228-231.
20. Lusso P., Gallo R.C. Human herpesvirus 6 in AIDS. Immunology Today 1995, 16(2):67-71.
21. Morissette G., Flamand L. Herpesviruses and chromosomal integration. Journal of Virology 2010, 84(23):12100-12109.
22. Nacheva E.P., Ward K.N., Brazma D., Virgili A., Howard J., Leong H.N., Clark D.A. Human herpesvirus 6 integrates within telomeric regions as evidenced by five different chromosomal sites. Journal of Medical Virology 2008, 80(11):1952-1958.
23. Neitzel H. A routine method for the establishment of permanent growing lymphoblastoid cell lines. Human Genetics 1986, 73(4):320-326.
24. Ogata M. Human herpesvirus 6 in hematological malignancies. Journal of Clinical and Experimental Hematology 2009, 49(2):57-67.
25. Pellett P.E., Ablashi D.V., Ambros P.F., Agut H., Caserta M.T., Descamps V., Flamand L., Gautheret-Dejean A., Hall C.B., Kamble R.T., Kuehl U., Lassner D., Lautenschlager I., Loomis K.S., Luppi M., Lusso P., Medveczky P.G., Montoya J.G., Mori Y., Ogata M., Pritchett J.C., Rogez S., Seto E., Ward K.N., Yoshikawa T., Razonable R.R. Chromosomally integrated human herpesvirus 6: questions and answers. Reviews in Medical Virology 2012, 22(3):144-155.
26. Raab-Traub N., Flynn K. The structure of the termini of the Epstein-Barr virus as a marker of clonal cellular proliferation. Cell 1986, 47(6):883-889.
27. Ravi D.S., Mitra D. HIV-1 long terminal repeat promoter regulated dual reporter: potential use in screening of transcription modulators. Analytical Biochemistry 2007, 360(2):315-317.
28. Santhosh C.V., Tamhane M.C., Kamat R.H., Patel V.V., Mukhopadhyaya R. A lentiviral vector with novel multiple cloning sites: stable transgene expression in vitro and in vivo. Biochemical and Biophysical Research Communications 2008, 371(3):546-550.
29. Tailor P.B., Saikia T.K., Advani S.H., Mukhopadhyaya R. Activation of HHV-6 in lymphoproliferative disorders: a polymerase chain reaction-based study. Annals of the New York Academy of Sciences 2004, 1022:282-285.
30. Tanaka-Taya K., Sashihara J., Kurahashi H., Amo K., Miyagawa H., Kondo K., Okada S., Yamanishi K. Human herpesvirus 6 (HHV-6) is transmitted from parent to child in an integrated form and characterization of cases with chromosomally integrated HHV-6 DNA. Journal of Medical Virology 2004, 73(3):465-473.
31. Telenti A., Marshall W.F., Smith T.F. Detection of Epstein-Barr virus by polymerase chain reaction. Journal of Clinical Microbiology 1990, 28(10):2187-2190.
32. Tham K.M., Chow V.T., Singh P., Tock E.P., Ching K.C., Lim-Tan S.K., Sng I.T., Bernard H.U. Diagnostic sensitivity of polymerase chain reaction and Southern blot hybridization for the detection of human papillomavirus DNA in biopsy specimens from cervical lesions. American Journal of Clinical Pathology 1991, 95(5):638-646.
33. Wang F.Z., Dahl H., Linde A., Brytting M., Ehrnst A., Ljungman P. Lymphotropic herpesviruses in allogeneic bone marrow transplantation. Blood 1996, 88(9):3615-3620.
34. Wang G.Q., Suzutani T., Yamamoto Y., Fukui Y., Nozawa N., Schmid D.S., Kurane I., Inoue N. Generation of a reporter cell line for detection of infectious varicella-zoster virus and its application to antiviral studies. Antimicrobial Agents and Chemotherapy 2006, 50(9):3142-3145.
35. Yamanishi K., Okuno T., Shiraki K., Takahashi M., Kondo T., Asano Y., Kurata T. Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet 1988, 1(8594):1065-1067.