A lentiviral vector with novel multiple cloning sites: Stable transgene expression in vitro and in vivo

Biochemical and Biophysical Research Communications

Volumn 371, Issue 3, 2008, Pages 546-550

  Santhosh, C V ^a   Tamhane, Mayur C ^a   Kamat, Rohan H ^a   Patel, Vainav V ^a   Mukhopadhyaya, Robin ^a  

^a Research and Education in Cancer ACTREC   (India)

Author keywords

HIV 2; In vivo transduction; Lentivirus vector; Multiple cloning sites; shRNA delivery; T A cloning; Transgene expression

Indexed keywords

GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR; SHORT HAIRPIN RNA;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL LINE; DOWN REGULATION; EMBRYO; FEMALE; GENE CASSETTE; GENE EXPRESSION; GENE TRANSFER; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 2; IN VITRO STUDY; IN VIVO STUDY; INDIAN; LIVER; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; NUDE MOUSE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SPLEEN; TRANSGENE; VIRUS ISOLATION;

ANIMALS; BASE SEQUENCE; CELL LINE; CLONING, MOLECULAR; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HIV-2; HUMANS; MICE; MICE, NUDE; MICRORNAS; MOLECULAR SEQUENCE DATA; TRANSGENES;

HUMAN IMMUNODEFICIENCY VIRUS 2; LENTIVIRUS; MUS MUSCULUS;

EID: 43549117460     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.04.106     Document Type: Article

References (20)

1. Kay M.A., Glorioso J.C., and Naldini L. Viral vectors for gene therapy: the art of turning infectious agents into vehicles of therapeutics. Nat. Med. 7 (2001) 33-40
2. Buchschacher Jr. G.L., and Wong-Staal F. Development of lentiviral vectors for gene therapy for human diseases. Blood 95 (2000) 2499-2504
3. Miyoshi H., Blomer U., Takahashi M., Gage F.H., and Verma I.M. Development of a self-inactivating lentivirus vector. J. Virol. 72 (1998) 8150-8157
4. Ikawa M., Tanaka N., Kao W.W., and Verma I.M. Generation of transgenic mice using lentiviral vectors: a novel preclinical assessment of lentiviral vectors for gene therapy. Mol. Ther. 8 (2003) 666-673
5. Saraga G., Mafficini A., Ghaneh P., Sorio C., and Costello E. Both HIV- and EIAV-based lentiviral vectors mediate gene delivery to pancreatic cancer cells and human pancreatic primary patient xenografts. Cancer Gene Ther. 14 (2007) 781-790
6. Mukherjee S., Lee H.L., Pacchia A.L., Ron Y., and Dougherty J.P. A HIV-2-based self-inactivating vector for enhanced gene transduction. J. Biotechnol. 127 (2007) 745-757
7. Tamhane M., Mukhopadhyaya R., and Mukhopadhyaya R. Characterization of a long terminal repeat region from an infectious Indian HIV type 2 isolate. AIDS Res. Hum. Retroviruses 21 (2005) 592-596
8. DeGottardi M.Q., Specht A., Metcalf B., Kaur A., Kirchhoff F., and Evans D.T. Selective downregulation of rhesus macaque and sooty mangabey major histocompatibility complex class I molecules by Nef alleles of simian immunodeficiency virus and human immunodeficiency virus type 2. J. Virol. 82 (2008) 3139-3146
9. Azevedo-Pereira J.M., Santos-Costa Q., and Moniz-Pereira J. HIV-2 infection and chemokine receptors usage-clues to reduced virulence of HIV-2. Curr. HIV Res. 3 (2005) 3-16
10. Noveen A., and Chuong C.M. Design of compact multiple cloning sites. Biotechniques 15 (1993) 208-214
11. Dull T., Zufferey R., Kelly M., Mandel R.J., Nguyen M., Trono D., and Naldini L. A third-generation lentivirus vector with a conditional packaging system. J. Virol. 72 (1998) 8463-8471
12. Soulas-Sprauel P., Le Guyader G., Rivera-Munoz P., Abramowski V., Olivier-Martin C., Goujet-Zalc C., Charneau P., and de Villartay J.P. Role for DNA repair factor XRCC4 in immunoglobulin class switch recombination. J. Exp. Med. 204 (2007) 1717-1727
13. Pei Y., and Tuschl T. On the art of identifying effective and specific siRNAs. Nat. Methods 3 (2006) 670-676
14. Paddison P.J., Caudy A.A., Sachidanandam R., and Hannon G.J. Short hairpin activated gene silencing in mammalian cells. Methods Mol. Biol. 265 (2004) 85-100
15. Frederick R.B., Ausbel M., Kingston R.E., Morre D.D., and Smith J.A. Current Protocols in Molecular Biology (2003), John Wiley and Sons, Inc., USA
16. Nguyen T.H., Oberholzer J., Birraux J., Majno P., Morel P., and Trono D. Highly efficient lentiviral vector-mediated transduction of nondividing, fully reimplantable primary hepatocytes. Mol. Ther. 6 (2002) 199-209
17. Cockrell A.S., and Kafri T. Gene delivery by lentivirus vectors. Mol. Biotechnol. 36 (2007) 184-204
18. Verhoeyen E., Wiznerowicz M., Olivier D., Izac B., Trono D., Dubart-Kupperschmitt A., and Cosset F.L. Novel lentiviral vectors displaying "early-acting cytokines" selectively promote survival and transduction of NOD/SCID repopulating human hematopoietic stem cells. Blood 106 (2005) 3386-3395
19. Poeschla E., Gilbert J., Li X., Huang S., Ho A., and Wong-Staal F. Identification of a human immunodeficiency virus type 2 (HIV-2) encapsidation determinant and transduction of nondividing human cells by HIV-2-based lentivirus vectors. J. Virol. 72 (1998) 6527-6536
20. Jackson A.L., Bartz S.R., Schelter J., Kobayashi S.V., Burchard J., Mao M., Li B., Cavet G., and Linsley P.S. Expression profiling reveals off-target gene regulation by RNAi. Nat. Biotechnol. 21 (2003) 635-637