Improving Griffith's protocol for co-extraction of microbial DNA and RNA in adsorptive soils

Soil Biology and Biochemistry

Volumn 63, Issue , 2013, Pages 37-49

  Paulin, Mélanie M ^a,c   Nicolaisen, Mette H ^a   Jacobsen, Carsten S ^a,b   Gimsing, Anne Louise ^a,d   Sørensen, Jan ^a   Bælum, Jacob ^b,e  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b GEOLOGICAL SURVEY OF DENMARK AND GREENLAND   (Denmark)

^c UNIVERSITÉ DE MONCTON   (Canada)

^d The Danish Environmental Protection Agency EPA   (Denmark)

^e Chalmers University of Technology   (Denmark)

Author keywords

Adsorption; DNA and RNA co extraction; High clay; Nucleic acid extraction; Quantitative PCR; Salmon sperm DNA

Indexed keywords

ACID EXTRACTION; EXTRACTION EFFICIENCIES; EXTRACTION PROTOCOLS; INDIGENOUS COMMUNITY; QUANTITATIVE PCR; QUANTITATIVE REAL TIME PCR; SOIL MICROBIAL COMMUNITY; TRANSCRIPT ANALYSIS;

ADSORPTION; BIOMOLECULES; DNA; GENE EXPRESSION; OPTIMIZATION; POLYMERASE CHAIN REACTION; PRECIPITATION (METEOROLOGY); RNA; SOILS;

EXTRACTION;

ADSORPTION; BIOMASS; DNA; GENE EXPRESSION; MICROBIAL COMMUNITY; MOLECULAR ANALYSIS; NUCLEIC ACID; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; RNA; SALMONID; SOIL MICROORGANISM; SPERM;

EID: 84876469893     PISSN: 00380717     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.soilbio.2013.02.007     Document Type: Article

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