Differences in amplification efficiency of standard curves in quantitative real-time PCR assays and consequences for gene quantification in environmental samples

Journal of Microbiological Methods

Volumn 82, Issue 3, 2010, Pages 338-341

  Töwe, Stefanie ^a   Kleineidam, Kristina ^b   Schloter, Michael ^b  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b INSTITUTE OF EPIDEMIOLOGY   (Germany)

Author keywords

Amplification efficiency; Functional genes; LinRegPCR; Nitrogen cycle; Quantitative real time PCR; Standard curve

Indexed keywords

FLUORESCENT DYE; NITRITE REDUCTASE; NITROGENASE; NITROUS OXIDE REDUCTASE; PLASMID DNA; UNSPECIFIC MONOOXYGENASE;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA EXTRACTION; DNA TEMPLATE; GENE DOSAGE; GENE TARGETING; GENETIC ANALYSIS; GENETIC DIFFERENCE; INTERMETHOD COMPARISON; MATHEMATICAL COMPUTING; MOLECULAR CLONING; NITROSOMONAS; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REPRODUCIBILITY; SOIL ANALYSIS;

EID: 77955655859     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.07.005     Document Type: Article

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