Flow cytometry enables a high-throughput homogeneous fluorescent antibody-binding assay for cytotoxic T cell lytic granule exocytosis

Journal of Biomolecular Screening

Volumn 18, Issue 4, 2013, Pages 420-429

  Florian, Amy E ^a   Lepensky, Christopher K ^a   Kwon, Ohyun ^b   Haynes, Mark K ^c   Sklar, Larry A ^c   Zweifach, Adam ^a  

^a UNIVERSITY OF CONNECTICUT   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF NEW MEXICO   (United States)

Author keywords

cytotoxic T lymphocytes; exocytosis; flow cytometry; high throughput screen; phenotypic assay

Indexed keywords

CALCIUM; INDOLE DERIVATIVE; LYSOSOME ASSOCIATED MEMBRANE PROTEIN 1; MITOGEN ACTIVATED PROTEIN KINASE; TETRACYANO HEXAHYDROISOINDOLE; UNCLASSIFIED DRUG;

ANTIGEN BINDING; ARTICLE; ASSAY; CALCIUM CELL LEVEL; CELL MEMBRANE DEPOLARIZATION; CHEMICAL STRUCTURE; CONTROLLED STUDY; CYTOTOXIC T LYMPHOCYTE; EXOCYTOSIS; FLOW CYTOMETRY; HIGH THROUGHPUT SCREENING; HOMOGENEOUS FLUORESCENT ANTIBODY BINDING ASSAY; HUMAN; HUMAN CELL; INHIBITION KINETICS; PRIORITY JOURNAL;

CELL LINE, TUMOR; DRUG EVALUATION, PRECLINICAL; EXOCYTOSIS; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; INDOLES; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEINS; SMALL MOLECULE LIBRARIES; T-LYMPHOCYTES, CYTOTOXIC;

EID: 84875755371     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057112466697     Document Type: Article

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