The role of platelet-rich plasma in tissue regeneration

Platelets

Volumn 24, Issue 3, 2013, Pages 173-182

  Alsousou, J ^a   Ali, A ^b   Willett, K ^a   Harrison, P ^c  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c CHURCHILL HOSPITAL   (United Kingdom)

Author keywords

Platelet; Platelet rich plasma; PRP; Regeneration; Tissue

Indexed keywords

EPIDERMAL GROWTH FACTOR; PLATELET DERIVED GROWTH FACTOR; PLATELET DERIVED GROWTH FACTOR RECEPTOR; SOMATOMEDIN; THROMBOCYTE FACTOR 4; TRANSFORMING GROWTH FACTOR BETA; VASCULOTROPIN;

ANTERIOR CRUCIATE LIGAMENT RECONSTRUCTION; BONE; CARTILAGE; CELL GROWTH; ERYTHROCYTE; HUMAN; LEUKOCYTE; NONHUMAN; PRIORITY JOURNAL; RANDOMIZED CONTROLLED TRIAL (TOPIC); RELATIVE DENSITY; REVIEW; THROMBOCYTE; THROMBOCYTE ACTIVATION; THROMBOCYTE POOR PLASMA; THROMBOCYTE RICH PLASMA; THROMBOCYTOPHERESIS; TISSUE REGENERATION; WOUND HEALING;

ANIMALS; GUIDED TISSUE REGENERATION; HUMANS; MODELS, ANIMAL; PLATELET-RICH PLASMA;

ANIMALIA;

EID: 84875733988     PISSN: 09537104     EISSN: 13691635     Source Type: Journal    
DOI: 10.3109/09537104.2012.684730     Document Type: Review

References (146)

1. Engebretsen L, Steffen K, Alsousou J, Anitua E, Bachl N, Devilee R, Everts P, Hamilton B, Huard J, Jenoure P, Kelberine F, Kon E, Maffulli N, Matheson G, Mei-Dan O, Menetrey J, Philippon M, Randelli P, Schamasch P, Schwellnus M, Vernec A, Verrall G. IOC consensus paper on the use of platelet-rich plasma in sports medicine. Br J Sports Med. 2010;44(15):1072-1081.
2. Nurden A. Platelets, Inflammation and tissue regeneration. Thromb Haemost. 2011;105:S13-S33.
3. Coppinger JA CG, Toomey S, et al. Charecterization of the protein released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions. Blood. 2004;103:2096-2104. (Pubitemid 38326225)
4. Harrison P, Cramer EM. Platelet alpha-granules. Blood Rev. 1993;7(1):52-62.
5. Gibble JW, Ness PM. Fibrin glue: The perfect operative sealant? Transfusion. 1990;30(8):741-747. (Pubitemid 20361794)
6. Sanchez AR, Sheridan PJ, Kupp LI. Is platelet-rich plasma the perfect enhancement factor? A current review. Int J Oral Maxillofac Implants. 2003;18(1):93-103. (Pubitemid 37140458)
7. Whitman DH, Berry RL, Green DM. Platelet gel: An autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg. 1997;55(11): 1294-1299. (Pubitemid 27495059)
8. Alsousou J, Thompson M, Hulley P, Noble A, Willett K. The biology of platelet-rich plasma and its application in trauma and orthopaedic surgery: A review of the literature. J Bone Joint Surg Br. 2009;91(8):987-996.
9. Gentile P, Cervelli V. Autologous platelet-rich plasma: Guidelines in plastic surgery. Plast Reconstr Surg. 2010;126(5): 269e-270e.
10. Pietrzak W, Eppley BL. Platelet rich plasma: Biology and new technology. Journal Craniofacial Surgery Journal Craniofacial Surgery. 2005;16(6):1043-1054. (Pubitemid 44383894)
11. Harrison PMI, Mumford A, Briggs C, Liesner R, Winter M, Machin S. BCSH guidelines for platelet function testing. British Journal of Haematology and BCSH website. 2011;155(1):30-44.
12. Kevy SVJM. Comparison of methods for point of care preparation of autologous platelet gel. The Journal of extracorporeal technology. 2004;36(1):28-35.
13. Landesberg RRM, Glickman RS. Quantification of growth factors levels using a simplified method of platelet rich plasma gel preparation. J Oral Maxillofac Surg. 2000;58:297-300. (Pubitemid 30129867)
14. Weibrich G, Kleis WK, Hafner G, Hitzler WE. Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count. J Craniomaxillofac Surg. 2002;30(2): 97-102. (Pubitemid 34846492)
15. Apher CSDW, Williamson LH, Sheckler VL, Fitzgerald BP. Platelet Collection with the Autopheresis-C apheresis system. Vox Sang. 1990;58:100-104.
16. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85(6):638-646. (Pubitemid 128641964)
17. Landesberg R, Moses M, Karpatkin M. Risks of using platelet rich plasma gel. J Oral Maxillofac Surg. 1998;56:1116-1998.
18. Cmolik BL, Spero J.A, Magovern G.J. Redo cardiac surgery: Late bleeding complications from topical thrombin induced factor five deficiency. J Thorac Cardiovasc Surg. 1993;105:222-227.
19. Anitua E, Andia I, Ardanza B, Nurden P, Nurden AT. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 2004;91(1):4-15. (Pubitemid 38111215)
20. Man D, Plosker H, Winland-Brown JE. The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg. 2001;107(1):229-237; discussion 38-9.
21. Han BW-MJ, Ponticiello M, Yang Z, Nimni M. The effect of Thrombin Activation of Platelet-Rish Plasma on Demineralized Bone Matrix Osteoinductivity. JBJS. 2009;91(6):1459-1470.
22. Harrison SVP, Kevy S, Jackobson M, Zurakowski D, Murray M. Platelet Activation by Collagen Provides Sustained Relsease of Anabolic Cytokines. Am J Sports Med. 2011;39(4):729-734.
23. Fufa D, Shealy B, Jacobson M, Kevy S, Murray MM. Activation of platelet-rich plasma using soluble type I collagen. J Oral Maxillofac Surg 2008;66:684-690. (Pubitemid 351381788)
24. Andia I, Sanchez M, Maffulli N. Tendon healing and platelet-rich plasma therapies. Expert Opinion on Biological Therapy. 2010;10(10):1415-1426.
25. Castelnovo L, Dosquet C, Gaudric A, Sahel J, Hicks D. Human platelet suspension stimulates porcine retinal glial proliferation and migration in vitro. Invest Ophthalmol Vis Sci. 2000;41(2): 601-609. (Pubitemid 30080467)
26. Kajikawa Y, Morihara T, Sakamoto H, Matsuda K, Oshima Y, Yoshida A, Nagae M, Arai Y, Kawata M, Kubo T. Platelet-rich plasma enhances the initial mobilization of circulation-derived cells for tendon healing. J Cell Physiol. 2008;215(3):837-845. (Pubitemid 351632705)
27. Bertrand-Duchesne MP, Grenier D, Gagnon G. Epidermal growth factor released from platelet-rich plasma promotes endothelial cell proliferation in vitro. J Periodontal Res. 2010;45(1):87-93.
28. Bertoldi C, Pinti M, Zaffe D, Cossarizza A, Consolo U, Ceccherelli GB. Morphologic, histochemical, and functional analysis of platelet-rich plasma activity on skeletal cultured cells. Transfusion. 2009;49(8):1728-1737.
29. Akeda K, An HS, Okuma M, Attawia M, Miyamoto K, Thonar EJ, Lenz ME, Sah RL, Masuda K. Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis. Osteoarthritis Cartilage. 2006;14(12):1272-1280. (Pubitemid 44774435)
30. Ishida K, Kuroda R, Miwa M, Tabata Y, Hokugo A, Kawamoto T, Sasaki K, Doita M, Kurosaka M. The regenerative effects of platelet-rich plasma on meniscal cells in vitro and its in vivo application with biodegradable gelatin hydrogel. Tissue Eng. 2007;13(5):1103-1112. (Pubitemid 46870434)
31. Pantou AL, Markopoulou CE, Dereka XE, Vavouraki H, Mamalis A, Vrotsos IA. The effect of platelet-rich plasma (PRP) combined with a bone allograft on human periodontal ligament (PDL) cells. Cell Tissue Bank. 2010 Dec 1.
32. Feng Y, Sun Y, Jia W, Zhang C. Platelet-rich plasma and 1,25(OH)2 vitamin D3 synergistically stimulate osteogenic differentiation of adult human mesenchymal stem cells. Biotechnol Lett. 2010;32(5):635-642.
33. Huang S, Wang Z. Influence of platelet-rich plasma on proliferation and osteogenic differentiation of skeletal muscle satellite cells: An in vitro study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;110(4):453-462.
34. Lucarelli E, Fini M, Beccheroni A, Giavaresi G, Di Bella C, Aldini NN, Guzzardella G, Martini L, Cenacchi A, Di Maggio N, Sangiorgi L, Fornasari PM, Mercuri M, Giardino R, Donati D. Stromal stem cells and platelet-rich plasma improve bone allograft integration. Clin Orthop Relat Res. 2005;June(435):62-68. (Pubitemid 40798647)
35. Lee JY, Nam H, Park YJ, Lee SJ, Chung CP, Han SB, Lee G. The effects of platelet-rich plasma derived from human umbilical cord blood on the osteogenic differentiation of human dental stem cells. In Vitro Cell Dev Biol Anim. 2011;47(2): 157-164.
36. Lee UL, Jeon SH, Park JY, Choung PH. Effect of platelet-rich plasma on dental stem cells derived from human impacted third molars. Regen Med. 2011;6(1):67-79.
37. Zhang J, Wang JH. Platelet-rich plasma releasate promotes differentiation of tendon stem cells into active tenocytes. Am J Sports Med. 2010;38(12):2477-2486.
38. Arpornmaeklong P, Kochel M, Depprich R, Kubler NR, Wurzler KK. Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study. Int J Oral Maxillofac Surg. 2004;33(1):60-70. (Pubitemid 38035495)
39. Soffer E, Ouhayoun JP, Dosquet C, Meunier A, Anagnostou F. Effects of platelet lysates on select bone cell functions. Clin Oral Implants Res. 2004;15(5):581-588. (Pubitemid 40104954)
40. van den Dolder J, Mooren R, Vloon AP, Stoelinga PJ, Jansen JA. Platelet-rich plasma: Quantification of growth factor levels and the effect on growth and differentiation of rat bone marrow cells. Tissue Eng. 2006;12(11):3067-3073. (Pubitemid 44846412)
41. Duan J, Kuang W, Tan J, Li H, Zhang Y, Hirotaka K, Tadashi K. Differential effects of platelet rich plasma and washed platelets on the proliferation of mouse MSC cells. Mol Biol Rep. 2011;38(4):2485-2490.
42. Creeper F, Lichanska AM, Marshall RI, Seymour GJ, Ivanovski S. The effect of platelet-rich plasma on osteoblast and periodontal ligament cell migration, proliferation and differentiation. J Periodontal Res. 2009;44(2):258-265.
43. Choi BH, Zhu SJ, Kim BY, Huh JY, Lee SH, Jung JH. Effect of platelet-rich plasma (PRP) concentration on the viability and proliferation of alveolar bone cells: An in vitro study. Int J Oral Maxillofac Surg. 2005;34(4):420-424.
44. Cheng M, Wang H, Yoshida R, Murray MM. Platelets and plasma proteins are both required to stimulate collagen gene expression by anterior cruciate ligament cells in three-dimensional culture. Tissue Eng Part A. 2010;16(5):1479-1489.
45. McCarrel T, Fortier L. Temporal growth factor release from platelet-rich plasma, trehalose lyophilized platelets, and bone marrow aspirate and their effect on tendon and ligament gene expression. J Orthop Res. 2009;27(8):1033-1042.
46. Schnabel LV, Mohammed HO, Miller BJ, McDermott WG, Jacobson MS, Santangelo KS, Fortier LA. Platelet rich plasma (PRP) enhances anabolic gene expression patterns in flexor digitorum superficialis tendons. J Orthop Res. 2007;25(2):230-240. (Pubitemid 46344249)
47. Lyras DN, Kazakos K, Tryfonidis M, Agrogiannis G, Botaitis S, Kokka A, Drosos G, Tilkeridis K, Verettas D. Temporal and spatial expression of TGF-beta1 in an Achilles tendon section model after application of platelet-rich plasma. Foot Ankle Surg. 2010;16(3):137-141.
48. Fallouh L, Nakagawa K, Sasho T, Arai M, Kitahara S, Wada Y, Moriya H, Takahashi K. Effects of autologous platelet-rich plasma on cell viability and collagen synthesis in injured human anterior cruciate ligament. J Bone Joint Surg Am. 2010;92(18):2909-2916.
49. Sánchez M, Anitua E, Azofra J, Prado R, Muruzabal F, Andia I. Ligamentization of Tendon Grafts Treated With an Endogenous Preparation Rich in Growth Factors: Gross Morphology and Histology. Arthroscopy: The Journal of Arthroscopic & Related Surgery. 2010;26(4):470-480.
50. Morizaki Y, Zhao C, An KN, Amadio PC. The effects of plateletrich plasma on bone marrow stromal cell transplants for tendon healing in vitro. J Hand Surg Am. 2010;35(11):1833-1841.
51. Tohidnezhad M, Varoga D, Wruck CJ, Brandenburg LO, Seekamp A, Shakibaei M, Sonmez TT, Pufe T, Lippross S. Platelet-released growth factors can accelerate tenocyte proliferation and activate the anti-oxidant response element. Histochem Cell Biol. 2011;135(5):453-460.
52. Lyras DN, Kazakos K, Georgiadis G, Mazis G, Middleton R, Richards S, O'Connor D, Agrogiannis G. Does a single application of PRP alter the expression of IGF-I in the early phase of tendon healing? J Foot Ankle Surg. 2011;50(3):276-282.
53. Lyras DN, Kazakos K, Agrogiannis G, Verettas D, Kokka A, Kiziridis G, Chronopoulos E, Tryfonidis M. Experimental study of tendon healing early phase: Is IGF-1 expression influenced by platelet rich plasma gel? Orthop Traumatol Surg Res. 2010;96(4):381-387.
54. Hu ZM, Peel SA, Ho SK, Sandor GK, Clokie CM. Comparison of platelet-rich plasma, bovine BMP, and rhBMP-4 on bone matrix protein expression in vitro. Growth Factors. 2009;27(5):280-288.
55. Tomoyasu A, Higashio K, Kanomata K, Goto M, Kodaira K, Serizawa H, et al. Platelet-rich plasma stimulates osteoblastic differentiation in the presence of BMPs. Biochem Biophys Res Commun. 2007;361(1):62-67. (Pubitemid 47102269)
56. Anitua E, And I, Sanchez M, Azofra J, del Mar Zalduendo M, de la Fuente M, Nurden P, Nurden AT. Autologous preparations rich in growth factors promote proliferation and induce VEGF and HGF production by human tendon cells in culture. Journal of Orthopaedic Research. 2005;23(2):281-286. (Pubitemid 40287850)
57. de Mos M, van der Windt AE, Jahr H, van Schie HT, Weinans H, Verhaar JA, van Osch GJ. Can platelet-rich plasma enhance tendon repair? A cell culture study. Am J Sports Med. 2008;36(6):1171-1178. (Pubitemid 351713047)
58. Lyras D, Kazakos K, Verettas D, Polychronidis A, Simopoulos C, Botaitis S, Agrogiannis G, Kokka A, Patsouris E. Immunohistochemical study of angiogenesis after local administration of platelet-rich plasma in a patellar tendon defect. Int Orthop. 2010;34(1):143-148.
59. Lyras DN, Kazakos K, Verettas D, Polychronidis A, Tryfonidis M, Botaitis S, Agrogiannis G, Simopoulos C, Kokka A, Patsouris E. The influence of platelet-rich plasma on angiogenesis during the early phase of tendon healing. Foot Ankle Int. 2009;30(11):1101-1106.
60. Slater M, Patava J, Kingham K, Mason RS. Involvement of platelets in stimulating osteogenic activity. J Orthop Res. 1995;13(5):655-663.
61. Oprea WE, Karp JM, Hosseini MM, Davies JE. Effect of platelet releasate on bone cell migration and recruitment in vitro. J Craniofac Surg. 2003;14(3):292-300.
62. Marx RE. Platelet-rich plasma (PRP): What is PRP and what is not PRP? Implant Dent 2001;10(4):225-228.
63. Marx RE. Platelet-rich plasma: Evidence to support its use. J Oral Maxillofac Surg. 2004;62(4):489-496. (Pubitemid 38451232)
64. Mariano R, Messora M, de Morais A, Nagata M, Furlaneto F, Avelino C, Paula F, Ferreira S, Pinheiro M, de Sene JP. Bone healing in critical-size defects treated with platelet-rich plasma: A histologic and histometric study in the calvaria of diabetic rat. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;109(1):72-78.
65. Kim ES, Park EJ, Choung PH. Platelet concentration and its effect on bone formation in calvarial defects: An experimental study in rabbits. J Prosthet Dent. 2001;86(4):428-433.
66. Pessoa RS, Oliveira SR, Menezes HH, de Magalhaes D. Effects of platelet-rich plasma on healing of alveolar socket: Split-mouth histological and histometric evaluation in Cebus apella monkeys. Indian J Dent Res. 2009;20(4):442-447.
67. Jungbluth P, Wild M, Grassmann JP, Ar E, Sager M, Herten M, Jager M, Becker J, Windolf J, Hakimi M. Platelet-rich plasma on calcium phosphate granules promotes metaphyseal bone healing in mini-pigs. J Orthop Res. 2010;28(11):1448-1455.
68. Fennis JP, Stoelinga PJ, Jansen JA. Mandibular reconstruction: A clinical and radiographic animal study on the use of autogenous scaffolds and platelet-rich plasma. Int J Oral Maxillofac Surg. 2002;31(3):281-286.
69. Kanthan SR, Kavitha G, Addi S, Choon DS, Kamarul T. Plateletrich plasma (PRP) enhances bone healing in non-united criticalsized defects: A preliminary study involving rabbit models. Injury. 2011;42(8):782-789.
70. Garcia RV, Gabrielli MA, Hochuli-Vieira E, Spolidorio LC, Filho JG, Neto FA, de Cardoso LA, Shibli JA. Effect of plateletrich plasma on peri-implant bone repair: A histologic study in dogs. J Oral Implantol. 2010;36(4):281-290.
71. Carvalho MD, Suaid FF, Santamaria MP, Casati MZ, Nociti Jr FH, Sallum AW, Sallum EA. Platelet-rich plasma plus bioactive glass in the treatment of intra-bony defects: A study in dogs. J Appl Oral Sci. 2011;19(1):82-89.
72. Kazakos K, Lyras DN, Thomaidis V, Agrogiannis G, Botaitis S, Drosos G, Kokka A, Verettas D. Application of PRP gel alone or in combination with guided bone regeneration does not enhance bone healing process: An experimental study in rabbits. J Craniomaxillofac Surg. 2011;39(1):49-53.
73. Ma L, Zheng LW, Sham MH, Lam CK, Zwahlen RA, Cheung LK. Effect of platelet-rich plasma on a rabbit model of nicotine-compromised bone healing. J Oral Maxillofac Surg. 2011;69(1):28-35.
74. Giovanini AF, Gonzaga CC, Zielak JC, Deliberador TM, Kuczera J, Goringher I, de Oliveira, Filho MA, Baratto-Filho F, Urban CA. Platelet-rich plasma (PRP) impairs the craniofacial bone repair associated with its elevated TGF-beta levels and modulates the co-expression between collagen III and alphasmooth muscle actin. J Orthop Res. 2011;29(3):457-463.
75. Broggini N, Hofstetter W, Hunziker E, Bosshardt DD, Bornstein MM, Seto I, Weibrich G, Buser D. The influence of PRP on early bone formation in membrane protected defects. A histological and histomorphometric study in the rabbit calvaria. Clin Implant Dent Relat Res. 2011;13(1):1-12.
76. Aspenberg P, Virchenko O. Platelet concentrate injection improves Achilles tendon repair in rats. Acta Orthop Scand. 2004;75(1):93-99. (Pubitemid 38264933)
77. Bosch G, van Schie HT, de Groot MW, Cadby JA, van de Lest CH, Barneveld A, van Weeren PR. Effects of platelet-rich plasma on the quality of repair of mechanically induced core lesions in equine superficial digital flexor tendons: A placebocontrolled experimental study. J Orthop Res. 2010;28(2):211-217.
78. Bosch G, Moleman M, Barneveld A, van Weeren PR, van Schie HT. The effect of platelet-rich plasma on the neovascularization of surgically created equine superficial digital flexor tendon lesions. Scand J Med Sci Sports. 2011;21(4):554-561.
79. Bir SC, Esaki J, Marui A, Sakaguchi H, Kevil CG, Ikeda T, Komeda M, Tabata Y, Sakata R. Therapeutic treatment with sustained-release platelet-rich plasma restores blood perfusion by augmenting ischemia-induced angiogenesis and arteriogenesis in diabetic mice. J Vasc Res. 2011;48(3):195-205.
80. Li NY, Yuan RT, Chen T, Chen LQ, Jin XM. Effect of plateletrich plasma and latissimus dorsi muscle flap on osteogenesis and vascularization of tissue-engineered bone in dogs. J Oral Maxillofac Surg. 2009;67(9):1850-1858.
81. Hadad I, Johnstone BH, Brabham JG, Blanton MW, Rogers PI, Fellers C, Solomon JL, Merfeld-Clauss S, DesRosiers CM, Dynlacht JR, Coleman JJ, March KL. Development of a porcine delayed wound-healing model and its use in testing a novel cell-based therapy. Int J Radiat Oncol Biol Phys. 2010;78(3):888-896.
82. Mishra A, Velotta J, Brinton TJ, Wang X, Chang S, Palmer O, Sheikh A, Chung J, Yang PC, Robbins R, Fischbein M. RevaTen platelet-rich plasma improves cardiac function after myocardial injury. Cardiovasc Revasc Med. 2011;12(3):158-163.
83. Nakamura S, Ishihara M, Takikawa M, Murakami K, Kishimoto S, Yanagibayashi S, Kubo S, Yamamoto N, Kiyosawa T. Platelet-rich plasma (PRP) promotes survival of fat-grafts in rats. Ann Plast Surg. 2010;65(1):101-106.
84. Pires Fraga MF, Nishio RT, Ishikawa RS, Perin LF, Helene Jr A, Malheiros CA. Increased survival of free fat grafts with plateletrich plasma in rabbits. J Plast Reconstr Aesthet Surg. 2010;63(12):e818-e822.
85. Rodriguez-Flores J, Palomar-Gallego MA, Enguita-Valls AB, Rodriguez-Peralto JL, Torres J. Influence of platelet-rich plasma on the histologic characteristics of the autologous fat graft to the upper lip of rabbits. Aesthetic Plast Surg. 2011;35(4):480-486.
86. Milano G, Sanna Passino E, Deriu L, Careddu G, Manunta L, Manunta A, Saccomanno MF, Fabbriciani C. The effect of platelet rich plasma combined with microfractures on the treatment of chondral defects: An experimental study in a sheep model. Osteoarthritis Cartilage. 2010;18(7):971-980.
87. DeRossi R, Coelho AC, Mello GS, Frazilio FO, Leal CR, Facco GG, Brum KB. Effects of platelet-rich plasma gel on skin healing in surgical wound in horses. Acta Cir Bras. 2009;24(4): 276-281.
88. Cho JM, Lee YH, Baek RM, Lee SW. Effect of platelet-rich plasma on ultraviolet b-induced skin wrinkles in nude mice. J Plast Reconstr Aesthet Surg. 2011;64(2):e31-e39.
89. Tanidir ST, Yuksel N, Altintas O, Yildiz DK, Sener E, Caglar Y. The effect of subconjunctival platelet-rich plasma on corneal epithelial wound healing. Cornea. 2010;29(6):664-669.
90. Matsuo R, Nakano Y, Ohkohchi N. Platelet administration via the portal vein promotes liver regeneration in rats after 70% hepatectomy. Ann Surg. 2011;253(4):759-763.
91. Yamaguchi R, Terashima H, Yoneyama S, Tadano S, Ohkohchi N. Effects of platelet-rich plasma on intestinal anastomotic healing in rats: PRP concentration is a key factor. J Surg Res. 2012;173(2):258-266.
92. Fresno L, Fondevila D, Bambo O, Chacaltana A, Garcia F, Andaluz A. Effects of platelet-rich plasma on intestinal wound healing in pigs. Vet J. 2010;185(3):322-327.
93. Yu W, Wang J, Yin J. Platelet-rich plasma: A promising product for treatment of peripheral nerve regeneration after nerve injury. Int J Neurosci. 2011;121(4):176-180.
94. Sanchez M, Anitua E, Azofra J, Andia I, Padilla S, Mujika I. Comparison of surgically repaired Achilles tendon tears using platelet-rich fibrin matrices. Am J Sports Med. 2007;35(2): 245-251. (Pubitemid 46145405)
95. Gaweda K, Tarczynska M, Krzyzanowski W. Treatment of Achilles tendinopathy with platelet-rich plasma. Int J Sports Med. 2010;31(8):577-583.
96. Kon E, Filardo G, Delcogliano M, Presti ML, Russo A, Bondi A, Di Martino A, Cenacchi A, Fornasari PM, Marcacci M. Plateletrich plasma: New clinical application: A pilot study for treatment of jumper's knee. Injury. 2009;40(6):598-603.
97. Volpi P, Quaglia A, Schoenhuber H, Melegati G, Corsi MM, Banfi G, de Girolamo L. Growth factors in the management of sport-induced tendinopathies: Results after 24 months from treatment. A pilot study. J Sports Med Phys Fitness. 2010;50(4):494-500.
98. Gosens T, Peerbooms JC, van Laar W, den Oudsten BL. Ongoing Positive Effect of Platelet-Rich Plasma Versus Corticosteroid Injection in Lateral Epicondylitis: A Double-Blind Randomized Controlled Trial With 2-year Follow-up. Am J Sports Med. 2011;39(6):1200-1208.
99. Creaney L, Wallace A, Curtis M, Connell D. Growth factorbased therapies provide additional benefit beyond physical therapy in resistant elbow tendinopathy: A prospective, doubleblind, randomised trial of autologous blood injections versus platelet-rich plasma injections. Br J Sports Med. 2011;45(12):966-971.
100. de Vos RJ, Weir A, van Schie HTM, Bierma-Zeinstra SMA, Verhaar JAN, Weinans H, Tol JL. Platelet-Rich Plasma Injection for Chronic Achilles Tendinopathy: A Randomized Controlled Trial. JAMA. 2010;303(2):144-149.
101. Schepull T, Kvist J, Norrman H, Trinks M, Berlin G, Aspenberg P. Autologous platelets have no effect on the healing of human achilles tendon ruptures: A randomized single-blind study. Am J Sports Med. 2011;39(1):38-47.
102. de Vos RJ, Weir A, Tol JL, Verhaar JA, Weinans H, van Schie HT. No effects of PRP on ultrasonographic tendon structure and neovascularisation in chronic midportion Achilles tendinopathy. Br J Sports Med. 2011;45(5):387-392.
103. de Vos RJ, van Veldhoven PL, Moen MH, Weir A, Tol JL, Maffulli N. Autologous growth factor injections in chronic tendinopathy: A systematic review. Br Med Bull. 2010;95:63-77.
104. Kampa RJ, Connell DA. Treatment of tendinopathy: Is there a role for autologous whole blood and platelet rich plasma injection? Int J Clin Pract 2010;64(13):1813-1823.
105. Vogrin M, Rupreht M, Dinevski D, Haspl M, Kuhta M, Jevsek M, Knezevic M, Rozman P. Effects of a platelet gel on early graft revascularization after anterior cruciate ligament reconstruction: A prospective, randomized, double-blind, clinical trial. Eur Surg Res. 2010;45(2):77-85.
106. Radice F, Yánez R, Gutiérrez V, Rosales J, Pinedo M, Coda S. Comparison of Magnetic Resonance Imaging Findings in Anterior Cruciate Ligament Grafts With and Without Autologous Platelet-Derived Growth Factors. Arthroscopy: The Journal of Arthroscopic & Related Surgery. 2010; 26(1):50-57.
107. Silva A, Sampaio R. Anatomic ACL reconstruction: Does the platelet-rich plasma accelerate tendon healing? Knee Surg Sports Traumatol Arthrosc 2009;17(6):676-682.
108. Vogrin M, Rupreht M, Crnjac A, Dinevski D, Krajnc Z, Recnik G. The effect of platelet-derived growth factors on knee stability after anterior cruciate ligament reconstruction: A prospective randomized clinical study. Wien Klin Wochenschr. 2010;122(Suppl 2):91-95.
109. Randelli PS, Arrigoni P, Cabitza P, Volpi P, Maffulli N. Autologous platelet rich plasma for arthroscopic rotator cuff repair. A pilot study. Disabil Rehabil. 2008;30(20-22): 1584-1589.
110. Castricini R, Longo UG, De Benedetto M, Panfoli N, Pirani P, Zini R, Maffulli N, Denaro V. Platelet-rich plasma augmentation for arthroscopic rotator cuff repair: A randomized controlled trial. Am J Sports Med. 2011;39(2):258-265.
111. Hamilton B, Knez W, Eirale C, Chalabi H. Platelet enriched plasma for acute muscle injury. Acta Orthop Belg. 2010;76(4): 443-448.
112. Bielecki T, Gazdzik TS, Szczepanski T. Benefit of percutaneous injection of autologous platelet-leukocyte-rich gel in patients with delayed union and nonunion. Eur Surg Res. 2008;40(3):289-296. (Pubitemid 351501598)
113. Lowery GL, Kulkarni S, Pennisi AE. Use of autologous growth factors in lumbar spinal fusion. Bone. 1999;25(2 Suppl):47S-50S. (Pubitemid 29333178)
114. Hartmann EK, Heintel T, Morrison RH, Weckbach A. Influence of platelet-rich plasma on the anterior fusion in spinal injuries: A qualitative and quantitative analysis using computer tomography. Arch Orthop Trauma Surg. 2010;130(7):909-914.
115. Landi A, Tarantino R, Marotta N, Ruggeri AG, Domenicucci M, Giudice L, Martini S, Rastelli M, Ferrazza G, De, Luca N, Tomei G, Delfini R. The use of platelet gel in postero-lateral fusion: Preliminary results in a series of 14 cases. Eur Spine J. 2011;20(Suppl 1):S61-S67.
116. Seijas R, Santana-Suarez RY, Garcia-Balletbo M, Cusco X, Ares O, Cugat R. Delayed union of the clavicle treated with plasma rich in growth factors. Acta Orthop Belg. 2010; 76(5):689-693.
117. Rodrigues SV, Acharya AB, Thakur SL. An evaluation of platelet-rich plasma without thrombin activation with or without anorganic bone mineral in the treatment of human periodontal intrabony defects. Platelets. 2011;22(5):353-360.
118. Nagaveni NB, Praveen RB, Umashankar KV, Pranav B, Sreedevi R, Radhika NB. Efficacy of platelet-rich-plasma (PRP) in bone regeneration after cyst enucleation in pediatric patients-a clinical study. J Clin Pediatr Dent. 2010;35(1): 81-87.
119. Scala M, Gipponi M, Mereu P, Strada P, Corvo R, Muraglia A, Massa M, Bertoglio S, Santi P, Cafiero F. Regeneration of mandibular osteoradionecrosis defect with platelet rich plasma gel. In Vivo. 2010;24(6):889-893.
120. Curi MM, Cossolin GS, Koga DH, Araujo SR, Feher O, dos Santos MO, Zardetto C. Treatment of avascular osteonecrosis of the mandible in cancer patients with a history of bisphosphonate therapy by combining bone resection and autologous plateletrich plasma: Report of 3 cases. J Oral Maxillofac Surg. 2007;65(2):349-355. (Pubitemid 46131421)
121. Cenni E, Fotia C, Rustemi E, Yuasa K, Caltavuturo G, Giunti A, Baldini N. Idiopathic and secondary osteonecrosis of the femoral head show different thrombophilic changes and normal or higher levels of platelet growth factors. Acta Orthop. 2011;82(1):42-49.
122. Cervelli V, Gentile P, De Angelis B, Calabrese C, Di Stefani A, Scioli MG, Curcio BC, Felici M, Orlandi A. Application of enhanced stromal vascular fraction and fat grafting mixed with PRP in post-traumatic lower extremity ulcers. Stem Cell Res. 2011;6(2):103-111.
123. Del Fabbro M, Bortolin M, Taschieri S, Weinstein R. Is platelet concentrate advantageous for the surgical treatment of periodontal diseases? A systematic review and meta-analysis. J Periodontol. 2011;82(8):1100-1111.
124. Kon E, Buda R, Filardo G, Di Martino A, Timoncini A, Cenacchi A, Fornasari PM, Giannini S, Marcacci M. Plateletrich plasma: Intra-articular knee injections produced favorable results on degenerative cartilage lesions. Knee Surg Sports Traumatol Arthrosc. 2010;18(4):472-479.
125. Filardo G, Kon E, Buda R, Timoncini A, Di Martino A, Cenacchi A, Fornasari PM, Giannini S, Marcacci M. Plateletrich plasma intra-articular knee injections for the treatment of degenerative cartilage lesions and osteoarthritis. Knee Surg Sports Traumatol Arthrosc. 2011;19(4):528-535.
126. Bullocks JM, Echo A, Guerra G, Stal S, Yuksel E. A novel autologous scaffold for diced-cartilage grafts in dorsal augmentation rhinoplasty. Aesthetic Plast Surg. 2011;35(4):569-579.
127. Lacci KM, Dardik A. Platelet-rich plasma: Support for its use in wound healing. Yale J Biol Med. 2010;83(1):1-9.
128. Crovetti G, Martinelli G, Issi M, Barone M, Guizzardi M, Campanati B, Moroni M, Carabelli A. Platelet gel for healing cutaneous chronic wounds. Transfus Apher Sci. 2004;30(2):145-151. (Pubitemid 38519799)
129. O'Connell SM, Impeduglia T, Hessler K, Wang XJ, Carroll RJ, Dardik H. Autologous platelet-rich fibrin matrix as cell therapy in the healing of chronic lower-extremity ulcers. Wound Repair Regen. 2008;16(6):749-756.
130. Driver VR, Hanft J, Fylling CP, Beriou JM. A prospective, randomized, controlled trial of autologous platelet-rich plasma gel for the treatment of diabetic foot ulcers. Ostomy Wound Manage. 2006;52(6):68-70, 2, 4 passim.
131. KrupskiWC, Reilly LM, Perez S, Moss KM, Crombleholme PA, Rapp JH. A prospective randomized trial of autologous plateletderived wound healing factors for treatment of chronic nonhealing wounds: A preliminary report. J Vasc Surg. 1991;14(4):526-532; discussion 32-6.
132. Frykberg RG, Driver VR, Carman D, Lucero B, Borris-Hale C, Fylling CP, Rappl LM, Clausen PA. Chronic wounds treated with a physiologically relevant concentration of platelet-rich plasma gel: A prospective case series. Ostomy Wound Manage. 2010;56(6):36-44.
133. Cervelli V, Lucarini L, Spallone D, Palla L, Colicchia GM, Gentile P, et al. Use of platelet-rich plasma and hyaluronic acid in the loss of substance with bone exposure. Adv Skin Wound Care. 2011;24(4):176-181.
134. Cervelli V, Lucarini L, Spallone D, Brinci L, de Angelis B. Use of platelet rich plasma and hyaluronic acid on exposed tendons of the foot and ankle. J Wound Care. 2010;19(5):186, 8-90.
135. Balbo R, Avonto I, Marenchino D, Maddalena L, Menardi G, Peano G. Platelet gel for the treatment of traumatic loss of finger substance. Blood Transfus. 2010;8(4):255-259.
136. Cieslik-Bielecka A, Bielecki T, Gazdzik TS, Arendt J, Krol W, Szczepanski T. Autologous platelets and leukocytes can improve healing of infected high-energy soft tissue injury. Transfus Apher Sci. 2009;41(1):9-12.
137. Lawlor DK, Derose G, Harris KA, Lovell MB, Novick TV, Forbes TL. The role of platelet-rich plasma in inguinal wound healing in vascular surgery patients. Vasc Endovascular Surg. 2011;45(3):241-245.
138. Almdahl SM, Veel T, Halvorsen P, Vold MB, Molstad P. Randomized prospective trial of saphenous vein harvest site infection after wound closure with and without topical application of autologous platelet-rich plasma. Eur J Cardiothorac Surg. 2011;39(1):44-48.
139. van der Hagen SJ, Baeten CG, Soeters PB, van Gemert WG. Autologous platelet-derived growth factors (platelet-rich plasma) as an adjunct to mucosal advancement flap in high cryptoglandular perianal fistulae: A pilot study. Colorectal Dis. 2011;13(2):215-218.
140. Casabona F, Priano V, Vallerino V, Cogliandro A, Lavagnino G. New surgical approach to lichen sclerosus of the vulva: The role of adipose-derived mesenchymal cells and platelet-rich plasma in tissue regeneration. Plast Reconstr Surg. 2010;126(4): 210e-211e.
141. Geremicca W, Fonte C, Vecchio S. Blood components for topical use in tissue regeneration: Evaluation of corneal lesions treated with platelet lysate and considerations on repair mechanisms. Blood Transfus. 2010;8(2):107-112.
142. Takikawa MNS, Ishirara M, Kishimoto S, Sasaki K, Yanagibayashi S, Azuma R, Kiyosawa T. Enhanced effect of Platelet Rich Plasma containing a new carrier on hair growth. Dermatol Surg. 2011;37(8):1721-1729.
143. Hee CK, Dines JS, Solchaga LA, Shah VR, Hollinger JO. Regenerative Tendon and Ligament Healing: Opportunities with Recombinant Human Platelet-Derived
144. Alsousou J FS, Hulley P, Thompson M, McNally E, Willett K. The Effect of Platelet Rich Plasma (PRP) on Tendon Regeneration: An in vitro Tissue Culture Study. International Symposium on Ligament and Tendon XI; Jan University of California: ISL&T; 2010. p. 58.
145. Alsousou J FS, Hulley P, Thompson M, McNally E, Willett K, editor. Clinical evidence platelet therapies in accelerating healing. UKA Symposium: Growth Factor Therapy in Elite Sports Medicine; 2009 25th November 2009; London.
146. Ljungqvist A, Schwellnus MP, Bachl N, Collins M, Cook J, Khan KM, Maffulli N, Pitsiladis Y, Riley G, Golspink G, Venter D, Derman EW, Engebretsen L, Volpi P. International Olympic Committee consensus statement: Molecular basis of connective tissue and muscle injuries in sport. Clin Sports Med. 2008;27:231-239.