Characterization of a novel thermostable chitin-binding domain and its application in immobilization of a multifunctional hemicellulase

Journal of Agricultural and Food Chemistry

Volumn 61, Issue 12, 2013, Pages 3074-3081

  Wang, Qilei ^a   Xue, Yemin ^a   Wu, Xixi ^a  

^a NANJING NORMAL UNIVERSITY   (China)

Author keywords

immobilization; multifunctional hemicellulase; thermostable chitin binding domain; xylose production

Indexed keywords

BINDING ACTIVITIES; C-TERMINAL REGIONS; CHITIN-BINDING DOMAINS; CONTINUOUS OPERATION; HEMICELLULASES; OPTIMUM TEMPERATURE; SIMULTANEOUS PURIFICATION; THERMOANAEROBACTERIUM;

CLONING; ENZYME IMMOBILIZATION; PURIFICATION; RADIOACTIVE WASTE VITRIFICATION; RECOMBINANT PROTEINS;

CHITIN;

CHITIN; GLYCOSIDASE; HEMICELLULASE; HYBRID PROTEIN; IMMOBILIZED ENZYME; MULTIFUNCTIONAL ENZYME; XYLOSE;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; BIOSYNTHESIS; CHEMISTRY; COMPARATIVE STUDY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; HEAT; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PH; THERMOANAEROBACTERIUM;

AMINO ACID SEQUENCE; BINDING SITES; CHITIN; CLONING, MOLECULAR; ENZYME STABILITY; ENZYMES, IMMOBILIZED; GLYCOSIDE HYDROLASES; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; MULTIFUNCTIONAL ENZYMES; RECOMBINANT FUSION PROTEINS; THERMOANAEROBACTERIUM; XYLOSE;

THERMOANAEROBACTERIUM; THERMOANAEROBACTERIUM THERMOSACCHAROLYTICUM;

EID: 84875715205     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf3041275     Document Type: Article

References (27)

1. Salemuddin, M. Bioaffinity based immobilization of enzymes Adv. Biochem. Eng. Biotechnol. 1999, 64, 203-226
2. Alison, R.; Gillies, Judy; Hsii, F.; Seachol, Oak; Wood, D. W. Rapid cloning and purification of proteins: gateway vectors for protein purification by self-cleaving tags Biotechnol. Bioeng. 2008, 2, 229-240
3. Wilhelm, T.; Frank, W. Immobilized enzymes: methods and applications Curr. Chem. 1999, 200, 95-126
4. Wu, W. Y.; Miller, K. D.; Michael, C.; Wood, D. W. Intein-mediated one-step purification of Escherichia coli secreted human antibody fragments Protein Expression Purif. 2011, 76, 221-228
5. Wood, D. W. Simplified protein purification using engineered self-cleaving affinity tags Chem. Technol. Biotechnol. 2003, 2-3, 103-110
6. Milton, T. W. H.; Danilo, A. Applications of novel affinity cassette methods: use of peptide fusion handles for the purification of recombinant proteins J. Mol. Recog. 2001, 14, 323-369
7. Kim, J. S.; Ronald, T. R. Peptide tags for a dual affinity fusion system Anal. Biochem. 1994, 219, 165-166
8. Majeti, N. V.; Kumar, R. A review of chitin and chitosan applications React. Funct. Polym. 2000, 46, 1-27
9. Chiang, C. J.; Wang, J. Y.; Chen, P. T.; Chao, Y. P. Enhanced levan production using chitin-binding domain fused levansucrase immobilized on chitin beads Appl. Microbiol. Biotechnol. 2009, 82, 445-451
10. Chern, J. T.; Chao, Y. P. Chitin-binding domain based immobilization of d -hydantoinase J. Biotechnol. 2005, 117, 267-275
11. Wang, J. Y.; Chao, Y. P. Immobilization of cells with surface-displayed chitin-binding domain Appl. Environ. Microbiol. 2006, 1, 927-931
12. Koji, N.; Michio, Y.; Mitsuyoshi, U. Production of autoproteolytically subunit-assembled 7-β-(4-carboxybutanamido) cephalosporanic acid (GL-7ACA) acylase from Pseudomonas sp. C427 using a chitin-binding domain Appl. Microbiol. Biotechnol. 2004, 65, 407-413
13. Chiang, C. J.; Chern, J. T.; Wang, J. Y.; Chao, Y. P. Facile immobilization of evolved Agrobacterium radiobacter carbamoylase with high thermal and oxidative stability J. Agric. Food Chem. 2008, 56, 6348-6354
14. Xue, Y. M.; Peng, J. J.; Wang, R. L.; Song, X. F. Construction of the trifunctional enzyme associating the Thermoanaerobacter ethanolicus xylosidase-arabinosidase with the Thermomyces lanuginosus xylanase for degradation of arabinoxylan Enzyme Microb. Technol. 2009, 45, 22-27
15. Wang, R.; Xue, Y.; Wu, X.; Song, X.; Peng, J. Enhancement of engineered trifunctional enzyme by optimizing linker peptides for degradation of agricultural by-products Enzyme Microb. Technol. 2010, 47, 194-199
16. Niehaus, F.; Bertoldo, C.; KaÈ hler, M.; Antranikian, G. Extremophiles as a source of novel enzymes for industrial application Appl. Microbiol. Biotechnol. Rev. 1999, 51, 711-729
17. Claire, V.; Gregory, J. Z. Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability Microbiol. Mol. Biol. Rev. 2001, 65, 1-43
18. Wang, S. Y.; Zhou, J. J.; Shao, B.; Lu, Y. J.; Raoa, P. F. Thermostable chitinase with chitin-binding activity from Phaseolus limensis J. Food Sci. 2008, 73 (6) 452-457
19. Jeuniaux, C. Chitinases Methods Enzymol. 1966, 8, 644-650
20. Sambrook, J.; Fritsch, E.; Maniatis, T. Molecular Cloning: A Laboratory Manual, 2 nd ed.; Cold Spring Harbor Laboratory Press, 1989.
21. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 1976, 72, 248-254
22. Lever, M. A new reaction for colorimetric determination of carbohydrates Anal. Biochem. 1972, 47, 273-279
23. Miller, G. L. Use of dinitrosalicyclic acid reagent for determination of reducing sugars Anal. Chem. 1959, 31, 426-428
24. Xue, Y.; Wu, A.; Zeng, H.; Shao, W. High-level expression of an α- l -arabinofuranosidase from thermotoga maritima in Escherichia coli for the production of xylobiose from xylan Biotechnol. Lett. 2006, 28, 351-356
25. Shao, W.; Xue, Y.; Wu, A.; Kataeva, I.; Jianjun Pei, J.; Wu, H.; Wiegel, J. Characterization of a novel β-xylosidase, XylC, from Thermoanaerobacterium saccharolyticum JW/SL-YS485 Appl. Environ. Microbiol. 2011, 3, 719-726
26. Koji, N.; Michio, Y.; Mitsuyoshi, U. Production of autoproteolytically subunit-assembled 7-β-(4-carboxybutanamido) cephalosporanic acid (GL-7ACA) acylase from Pseudomonas sp. C427 using a chitin-binding domain Appl. Microbiol. Biotechnol. 2004, 65, 407-413
27. Xue, Y. M.; Song, X. F.; Yu, J. J. Overexpression of β-glucosidase from Thermotoga maritima for the production of highly purified aglycone isoflavones from soy flour World J. Microbiol. Biotechnol. 2009, 25, 2165-2172