Engineering the loops in a lipase for stability in DMSO

Protein Engineering, Design and Selection

Volumn 26, Issue 4, 2013, Pages 317-324

  Yedavalli, Poornima ^a   Madhusudhana Rao, Nalam ^a  

^a CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

Author keywords

Bacillus subtilis lipase; dimethyl sulfoxide; loops; site saturation mutagenesis

Indexed keywords

BACILLUS SUBTILIS; CATALYTIC TURNOVER; DIMETHYL SULFOXIDE (DMSO); FLUORESCENT PROBES; LOOPS; SATURATION MUTAGENESIS; SECONDARY STRUCTURES; TERTIARY STRUCTURES;

AMINO ACIDS; DIMETHYL SULFOXIDE; MUTAGENESIS; PROTEINS;

ORGANIC SOLVENTS;

DIMETHYL SULFOXIDE; FLUORESCENT DYE; TRIACYLGLYCEROL LIPASE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME STABILITY; ENZYME STRUCTURE; GENE MUTATION; HYDROPHOBICITY; MELTING POINT; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

BACILLUS SUBTILIS; DIMETHYL SULFOXIDE; LIPASE; MODELS, MOLECULAR; MUTAGENESIS; MUTATION; PROTEIN ENGINEERING; PROTEIN STRUCTURE, SECONDARY; SOLVENTS;

BACILLUS SUBTILIS;

EID: 84875674252     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzt002     Document Type: Article

References (39)

1. Acharya,P., Rajakumara,E., Sankaranarayanan,R. and Rao,N.M. (2004) J. Mol. Biol., 341, 1271-1281.
2. Ahmad,S., Kamal,M.Z., Sankaranarayan,R. and Rao,N.M. (2008) J. Mol. Biol., 38, 324-340.
3. Ahmad,S., Kumar,V., Ramanand,K.B. and Rao,N.M. (2012) Protein Sci., 21, 433-436.
4. Arnold,F.H. (1990) Trends Biotechnol., 8, 244-249.
5. Brustad,E.M. and Arnold,F.H. (2011) Cur. Opin. Chem. Biol., 15, 201-210.
6. Chen,K. and Arnold,F.H. (1991) Biotechnology, 9, 1073-1077.
7. Chen,K. and Arnold,F.H. (1993) Proc Natl Acad Sci U. S. A., 90, 5618-5622.
8. Cobb,R.E., Si,T. and Zhao,H. (2012) Cur. Opin. Chem. Biol., 16, 285-291.
9. Farinas,E.T., Bulter,T. and Arnold,F.H. (2001) Curr. Opin. Biotechnol., 12, 545-551.
10. Haring,D. and Schreier,P. (1999) Cur. Opin. Chem. Biol., 3, 35, 38.
11. Hutcheon,G.A., Halling,P.J. and Moore,B.D. (2000) In Rubin,B. and Dennis,E.A. (eds), Book Series: Methods in Enzymology, Vol. 286. New York: Academic Press., pp. 465-72.
12. Kamal,M.Z., Mohammad,T.A.S., Krishnamoorthy,G. and Rao,N.M. (2012) PlosOne, 7, e35188.
13. Kawasaki,K., Kondo,H., Suzuki,M., Ohgiya,S. and Tsuda,S. (2002) Acta Crystallogr., Sect. D, 58, 1168-1174.
14. Kawata,T. and Ogino,H. (2009) Biotechnol. Prog., 25, 1605-1611.
15. Kelly,S.M. and Price,N.C. (1997) Biochim. Biophys. Acta., 1338, 161-167.
16. Klibanov,A.M. (1989) Trends Biochem. Sci., 14, 141-144.
17. Klibanov,A.M. (1997) Trend Biotech., 15, 97-101.
18. Klibanov,A.M. (2001) Nature, 409, 241-246.
19. Kumar,S., Sun,L., Liu,H., Muralidhara,B.K. and Halpert,J.R. (2006) Protein Eng. Des. Select., 19, 547-554.
20. Liao,H., McKenzie,T. and Hageman,R. (1986) Proc. Natl. Acad. Sci. U. S. A., 83, 576-580.
21. Markwell,M.A.K., Haas,S.M., Bieber,L.L. and Tolbert,N.E. (1978) Anal. Biochem., 87, 206-210.
22. Ogino,H. and Ishikawa,H. (2001) J. Biosci. Bioeng., 91, 109-116.
23. Oliphant,A.R. and Struhl,K. (1989) Proc. Natl. Acad. Sci. U. S. A., 86, 9094-9098.
24. Reetz,M.T., D Carballeira,J. and Vogel,A. (2006) Angewandte Chemie, 45, 7745-7751.
25. Reetz,M.T., Bocola,M., Wang,L., et al. (2009) J. Am. Chem. Soc., 131, 7334-7343.
26. Roccatano,D., Wong,T.S., Schwaneberg,U. and Zacharias,M. (2006) Biopolymers, 83, 467-476.
27. Ru,M.J., Dordick,J.S. and Clark,D.S. (1999) Biotech. Bioeng., 63, 233-241.
28. Serdakowski,A.L. and Dordick,J.S. (2007) Trends Biotech., 26, 48-54.
29. Sharma,K.K., Kaur,H., Kumar,G.S. and Kester,K. (1998) J. Biol. Chem., 273, 8965-8970.
30. Smoot,A.L., Panda,M., Brazil,B.T., Buckle,A.M., Fersht,A.R. and Horowitz,P.M. (2001) Biochemistry, 40, 4484-4492.
31. Song,J.K. and Rhee,J.S. (2001) Biochim. Biophys. Acta, 1547, 370-378.
32. Timasheff,S.N. (1993) Ann. Rev. Biophys. Biomol. Struc., 22, 67-97.
33. Trodler,P. and Pleiss,J. (2008) BMC Struc. Biol., 8, 9.
34. Turner,N.J. (2009) Nat. Chem. Biol., 5, 567-577.
35. Van Pouderoyen,G., Eggert,T., Jaeger,K.E. and Dijkstra,B.W. (2001) J. Mol. Biol., 309, 215-226.
36. Wintrode,P.L. and Arnold,F.H. (2001) Adv. Protein Chem., 55, 161-225.
37. Wohlgemuth,R. (2010) Cur. Opin. Biotech., 21, 713-724.
38. Wong,T.S., Arnold,F.H. and Schwaneberg,U. (2004) Biotechnol. Bioeng., 85, 351-358.
39. Zumarraga,M., Bulter,T., Shleev,S., Polaina,J., Martinez-Arias,A., Plou,F.J., Ballesteros,A. and Alcalde,M. (2007) Chem. Biol., 14, 1052-1064.