Enhancement of stability and activity of phospholipase A1 in organic solvents by directed evolution

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1547, Issue 2, 2001, Pages 370-378

  Song, Jae Kwang ^a   Rhee, Joon Shick ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Catalytic activity; Directed evolution; Organic solvent; Phospholipase A1; Stability

Indexed keywords

DIMETHYL SULFOXIDE; DNA; ENZYME VARIANT; MUTANT PROTEIN; ORGANIC SOLVENT; PHOSPHATIDYLCHOLINE; PHOSPHOLIPASE A1;

ANALYTIC METHOD; ARTICLE; CATALYSIS; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVITY; ENZYME STABILITY; GEL; GENE ISOLATION; GENE LIBRARY; GENE MUTATION; HALF LIFE TIME; NONHUMAN; PRIORITY JOURNAL; PRODUCTIVITY; SCREENING;

AMINO ACID SUBSTITUTION; CATALYSIS; ENZYME ACTIVATION; ENZYME STABILITY; KINETICS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHOSPHOLIPASES A; SOLVENTS;

EID: 0035844691     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(01)00204-7     Document Type: Article

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