Development of a Fur-dependent and tightly regulated expression system in Escherichia coli for toxic protein synthesis

BMC Biotechnology

Volumn 13, Issue , 2013, Pages

  Guan, Lingyu ^a   Liu, Qin ^a   Li, Chao ^a   Zhang, Yuanxing ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL CONTAINMENT; BIOTECHNOLOGY APPLICATIONS; EXPRESSION VECTORS; PROGRAMMED CELL DEATHS; PROKARYOTIC EXPRESSION SYSTEM; PROMOTER SEQUENCES; PROTEIN PRODUCTION LEVELS; VACCINE DEVELOPMENT;

BIOSYNTHESIS; CELL DEATH; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; IRON; IRON COMPOUNDS;

PROTEINS;

ESCHERICHIA COLI PROTEIN; FERRIC UPTAKE REGULATOR; FERROUS ION; GEF PROTEIN; GREEN FLUORESCENT PROTEIN; IRON; MAZF PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; FLUORESCENCE; GENE DOSAGE; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; GENE SEQUENCE; GENETIC SCREENING; IRON BINDING CAPACITY; IRON TRANSPORT; NONHUMAN; PROMOTER REGION; PROTEIN SYNTHESIS; REPORTER GENE;

2,2'-DIPYRIDYL; BACTERIAL PROTEINS; BACTERIAL TOXINS; CLONING, MOLECULAR; DNA-BINDING PROTEINS; ENDORIBONUCLEASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; IRON; IRON CHELATING AGENTS; MEMBRANE PROTEINS; PLASMIDS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; REPRESSOR PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA;

EID: 84875018347     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-13-25     Document Type: Article

References (35)

1. Kimelman A, Levy A, Sberro H, Kidron S, Leavitt A, Amitai G, Yoder-Himes DR, Wurtzel O, Zhu Y, Rubin EM. A vast collection of microbial genes that are toxic to bacteria. Genome Res 2012, 22(9):1-8. 3246196, 22147369.
2. Singh JS, Abhilash P, Singh H, Singh RP, Singh D. Genetically engineered bacteria: An emerging tool for environmental remediation and future research perspectives. Gene 2011, 480(1-2):1-9.
3. Atlas RM. Molecular methods for environmental monitoring and containment of genetically engineered microorganisms. Biodegradation 1992, 3(2-3):137-146.
4. Molin S. Environmental potential of suicide genes. Curr Opin Biotechnol 1993, 4(3):299-305. 10.1016/0958-1669(93)90099-I, 7763722.
5. Saida F, Uzan M, Odaert B, Bontems F. Expression of highly toxic genes in E. coli: special strategies and genetic tools. Curr Protein Pept Sci 2006, 7(1):47-56. 10.2174/138920306775474095, 16472168.
6. Jana S, Deb J. Strategies for efficient production of heterologous proteins in Escherichia coli. Appl Microbiol Biotechnol 2005, 67(3):289-298. 10.1007/s00253-004-1814-0, 15635462.
7. Choi YJ, Morel L, Le Francois T, Bourque D, Bourget L, Groleau D, Massie B, Miguez CB. Novel, versatile, and tightly regulated expression system for Escherichia coli strains. Appl Environ Microbiol 2010, 76(15):5058-5066. 10.1128/AEM.00413-10, 2916507, 20562288.
8. Giacalone MJ, Gentile AM, Lovitt BT, Berkley NL, Gunderson CW, Surber MW. Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system. Biotechniques 2006, 40(3):355-363. 10.2144/000112112, 16568824.
9. Glascock C, J Weickert M Using chromosomal lacIQ1 to control expression of genes on high-copy-number plasmids in Escherichia coli. Gene 1998, 223(1):221-231. 10.1016/S0378-1119(98)00240-6, 9858738, J Weickert M.
10. Royo JL, Manyani H, Cebolla A, Santero E. A new generation of vectors with increased induction ratios by overimposing a second regulatory level by attenuation. Nucleic Acids Res 2005, 33(19):e169-e169. 10.1093/nar/gni168, 1275594, 16260471.
11. Anthony LC, Suzuki H, Filutowicz M. Tightly regulated vectors for the cloning and expression of toxic genes. J Microbiol Methods 2004, 58(2):243-250. 10.1016/j.mimet.2004.04.003, 15234522.
12. Voigt CA. Genetic parts to program bacteria. Curr Opin Biotechnol 2006, 17(5):548-557. 10.1016/j.copbio.2006.09.001, 16978856.
13. Allen CE, Schmitt MP. HtaA is an iron-regulated hemin binding protein involved in the utilization of heme iron in Corynebacterium diphtheriae. J Bacteriol 2009, 191(8):2638-2648. 10.1128/JB.01784-08, 2668399, 19201805.
14. Hoffelner H, Rieder G, Haas R. Helicobacter pylori vaccine development: Optimisation of strategies and importance of challenging strain and animal model. Int J Med Microbiol 2008, 298(1-2):151-159.
15. Lim JM, Hong M, Kim S, Oh D, Kang H, Kwon O. Iron chelator-inducible expression system for Escherichia coli. J Microbiol Biotechnol 2008, 18(8):1357-1363.
16. Baichoo N, Helmann JD. Recognition of DNA by Fur: a reinterpretation of the Fur box consensus sequence. J Bacteriol 2002, 184(21):5826-5832. 10.1128/JB.184.21.5826-5832.2002, 135393, 12374814.
17. Escolar L, Pérez-Martín J, de Lorenzo V. Binding of the Fur (ferric uptake regulator) repressor of Escherichia coli to arrays of the GATAAT sequence. J Mol Biol 1998, 283(3):537-547. 10.1006/jmbi.1998.2119, 9784364.
18. Guan LY, Mu W, Champeimont J, Wang QY, HZh W, Xiao JF, Lubitz W, Zhang YX, Liu Q. Iron-regulated lysis of recombinant Escherichia coli in host releases protective antigen and confers biological containment. Infect Immun 2011, 79(7):2608-2618. 10.1128/IAI.01219-10, 3191992, 21536797.
19. Rogers MB, Sexton JA, DeCastro GJ, Calderwood SB. Identification of an operon required for ferrichrome iron utilization in Vibrio cholerae. J Bacteriol 2000, 182(8):2350-2353. 10.1128/JB.182.8.2350-2353.2000, 111292, 10735886.
20. Mey AR, Wyckoff EE, Kanukurthy V, Fisher CR, Payne SM. Iron and fur regulation in Vibrio cholerae and the role of fur in virulence. Infect Immun 2005, 73(12):8167-8178. 10.1128/IAI.73.12.8167-8178.2005, 1307094, 16299312.
21. Xiao Y, Liu Q, Chen H, Zhang Y. A stable plasmid system for heterologous antigen expression in attenuated Vibrio anguillarum. Vaccine 2011, 29(40):6986-6993. 10.1016/j.vaccine.2011.07.036, 21791231.
22. Witte A, Wanner G, Sulzner M, Lubitz W. Dynamics of PhiX174 protein E-mediated lysis of Escherichia coli. Arch Microbiol 1992, 157(4):381-388. 10.1007/BF00248685, 1534215.
23. Gerdes K, Poulsen L, Thisted T, Nielsen A, Martinussen J, Andreasen P. The hok killer gene family in gram-negative bacteria. New Biol 1990, 2(11):946-956.
24. Amitai S, Yassin Y, Engelberg-Kulka H. MazF-mediated cell death in Escherichia coli: a point of no return. J Bacteriol 2004, 186(24):8295-8300. 10.1128/JB.186.24.8295-8300.2004, 532418, 15576778.
25. Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E. Improved Expression Systems for Regulated Expression in Salmonella Infecting Eukaryotic Cells. PLoS One 2011, 6(8):e23055. 10.1371/journal.pone.0023055, 3148252, 21829692.
26. Stumm W, Morgan JJ. Aquatic chemistry 1996, New York, USA: John Wiley & Sons, 3.
27. Mu W, Guan L, Yan Y, Liu Q, Zhang Y. A novel in vivo inducible expression system in Edwardsiella tarda for potential application in bacterial polyvalence vaccine. Fish Shellfish Immunol 2011, 31(6):1097-1105. 10.1016/j.fsi.2011.09.015, 21964456.
28. Loessner H, Endmann A, Leschner S, Bauer H, Zelmer A, Westphal K, Weiss S, zur Lage S Improving live attenuated bacterial carriers for vaccination and therapy. Int J Med Microbiol 2008, 298(1-2):21-26. zur Lage S.
29. Wang S, Li Y, Shi H, Sun W, Roland KL, Curtiss R. Comparison of a regulated delayed antigen synthesis system with in vivo-inducible promoters for antigen delivery by live attenuated Salmonella vaccines. Infect Immun 2011, 79(2):937-949. 10.1128/IAI.00445-10, 3028866, 21134969.
30. John M, Crean TI, Calderwood SB, Ryan ET. In vitro and in vivo analyses of constitutive and in vivo-induced promoters in attenuated vaccine and vector strains of Vibrio cholerae. Infect Immun 2000, 68(3):1171-1175. 10.1128/IAI.68.3.1171-1175.2000, 97263, 10678922.
31. Morin CE, Kaper JB. Use of stabilized luciferase-expressing plasmids to examine in vivo-induced promoters in the Vibrio cholerae vaccine strain CVD 103-HgR. FEMS Immunol Med Mic 2009, 57(1):69-79.
32. Husseiny MI, Hensel M. Evaluation of an intracellular-activated promoter for the generation of live Salmonella recombinant vaccines. Vaccine 2005, 23(20):2580-2590. 10.1016/j.vaccine.2004.11.035, 15780440.
33. Curtiss R, Xin W, Li Y, Kong W, Wanda S, Gunn B, Wang S. New technologies in using recombinant attenuated Salmonella vaccine vectors. Crit Rev Immunol 2010, 30(3):255-270. 10.1615/CritRevImmunol.v30.i3.30, 20370633.
34. Miller JH. Experiments in molecular genetics 1972, Cold Spring Harbor, New York, USA: Cold Spring Harbor Laboratory.
35. Carlson JM, Chakravarty A, DeZiel CE. SCOPE: a web server for practical de novo motif discovery. Nucleic Acids Res 2007, 35(suppl 2):W259-W264. 1933170, 17485471.