A new generation of vectors with increased induction ratios by overimposing a second regulatory level by attenuation

Nucleic Acids Research

Volumn 33, Issue 19, 2005, Pages 1-10

  Royo, Jose Luis ^a   Manyani, Hamid ^b   Cebolla, Angel ^b   Santero, Eduardo ^a  

^a UNIVERSIDAD PABLO DE OLAVIDE   (Spain)

^b Biomedal SL   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL GENETICS; CONTROLLED STUDY; EXPRESSION VECTOR; GENE EXPRESSION REGULATION; KLEBSIELLA OXYTOCA; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS PUTIDA; TRANSCRIPTION REGULATION; GENE SILENCING; GENE VECTOR; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; OPERON; SILENCER ELEMENT;

BACTERIA (MICROORGANISMS); KLEBSIELLA OXYTOCA; PSEUDOMONAS PUTIDA;

ANTITERMINATOR PROTEINS, BACTERIA; BACTERIAL PROTEIN; DNA BINDING PROTEIN; NAHR PROTEIN, BACTERIA; RNA BINDING PROTEIN; TRANSACTIVATOR PROTEIN; TRANSCRIPTION FACTOR; XYLS PROTEIN, PSEUDOMONAS PUTIDA;

BACTERIAL PROTEINS; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENE SILENCING; GENETIC VECTORS; KLEBSIELLA OXYTOCA; OPERON; PSEUDOMONAS PUTIDA; RNA-BINDING PROTEINS; SILENCER ELEMENTS, TRANSCRIPTIONAL; TRANS-ACTIVATORS; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

EID: 28544450524     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gni168     Document Type: Article

References (37)

1. Jonasson,P., Liljeqvist,S., Nygren,P.A. and Stahl,S. (2002) Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli. Biotechnol. Appl. Biochem., 35, 91-105.
2. Drew,D., Froderberg,L., Baars,L. and de Grier,J.W. (2003) Assembly and overexpression of membrane proteins in Escherichia coli. Biochim. Biophys. Acta, 1610, 3-10.
3. Mertens,N., Remaut,E. and Fiers,W. (1995) Tight transcriptional control mechanism ensures stable high-level expression from T7 promoter-based expression plasmids. Biotechnology, 13, 175-179.
4. Cebolla,A., Royo,J.L., De Lorenzo,V. and Santero,E. (2002) Improvement of recombinant protein yield by a combination of transcriptional amplification and stabilization of gene expression. Appl. Environ. Microbiol., 68, 5034-5041.
5. Keasling,J.D. (1999) Gene-expression tools for the metabolic engineering of bacteria. Trends Biotechnol., 17, 452-460.
6. Sninsky,J.J., Uhlin,B.E., Gustafsson,P. and Cohen,S.N. (1981) Construction and characterization of a novel two-plasmid system for accomplishing temperature-regulated, amplified expression of cloned adventitious genes in Escherichia coli. Gene, 16, 275-286.
7. Chen,W., Kallio,P.T. and Bailey,J.E. (1993) Construction and characterization of a novel cross-regulation system for regulating cloned gene expression in Escherichia coli. Gene, 130, 15-22.
8. Baneyx,F. (1999) Recombinant protein expression in Escherichia coli. Curr. Opin. Biotechnol., 10, 411-421.
9. Cebolla,A., Sousa,C. and de Lorenzo,V. (2001) Rational design of a bacterial transcriptional cascade for amplifying gene expression capacity. Nucleic Acids Res., 29, 759-766.
10. Iost,I. and Dreyfus,M. (1995) The stability of Escherichia coli lacZ mRNA depends upon the simultaneity of its synthesis and translation. EMBO J., 14, 3252-3261.
11. Carrier,T.A. and Keasling,J.D. (1999) Library of synthetic 5′ secondary structures to manipulate mRNA stability in Escherichia coli. Biotechnol. Prog., 15, 58-64.
12. Corisdeo,S. and Wang,B. (2004) Functional expression and display of an antibody Fab fragment in Escherichia coli: Study of vector designs and culture conditions. Protein Expr. Purif., 34, 270-279.
13. Hui,A., Hayflick,J., Dinkelspiel,K. and de Boer,H.A. (1984) Mutagenesis of the three bases preceding the start codon of the beta-galactosidase mRNA and its effect on translation in Escherichia coli. EMBO J., 3, 623-629.
14. Alexander,D.M., Hesson,T., Mannarino,A., Cable,M. and Dalie,B.L. (1992) Isolation and purification of a biologically active human platelet-derived growth factor BB expressed in Escherichia coli. Protein Expr. Purif., 3, 204-211.
15. Merino,E. and Yanofsky,C. (2005) Transcription attenuation: A highly conserved regulatory strategy used by bacteria. Trends Genet., 21, 260-264.
16. Gollnick,P. and Babitzke,P. (2002) Transcription attenuation. Biochim. Biophys. Acta, 1577, 240-250.
17. Elf,J., Berg,O.G. and Ehrenberg,M. (2001) Comparison of repressor and transcriptional attenuator systems for control of amino acid biosynthetic operons. J. Mol. Biol., 313, 941-954.
18. Weisberg,R.A. and Gottesman,M.E. (1999) Processive antitermination. J. Bacteriol., 181, 359-367.
19. Rutberg,B. (1997) Antitermination of transcription of catabolic operons. Mol. Microbiol., 23, 413-421.
20. Lin,J.T. and Stewart,V. (1996) Nitrate and nitrite-mediated transcription antitermination control of nasF (nitrate assimilation) operon expression in Klebsiella pneumoniae M5al. J. Mol. Biol., 256, 423-435.
21. Goldmann,B.S., Lin,J.T. and Stewart,V. (1994) Identification and structure of the nasR gene encoding a nitrate- and nitrite-responsive positive regulator of nasFEDCBA (nitrate assimilation) operon expression in Klebsiella pneumoniae M5al. J. Bacteriol., 176, 5077-5085.
22. Chai,W. and Stewart,V. (1998) NasR, a novel RNA-binding protein, mediates nitrate-responsive transcription antitermination of the Klebsiella oxytoca M5al nasF operon leader in vitro. J. Mol. Biol., 283, 339-351.
23. Chai,W. and Stewart,V. (1999) RNA sequence requirements for NasR-mediated, nitrate-responsive transcription antitermination of the Klebsiella oxytoca M5al nasF operon leader. J. Mol. Biol., 292, 203-216.
24. Wu,S.Q., Chai,W., Lin,J.T. and Stewart,V. (1999) General nitrogen regulation of nitrate assimilation regulatory gene nasR expression in Klebsiella oxytoca M5al. J. Bacteriol., 181, 7274-7284.
25. Royo,J.L., Moreno-Ruiz,E., Cebolla,A. and Santero,E. (2005) Stable long-term indigo production by overexpression of dioxygenase genes using a chromosomal integrated cascade expression circuit. J. Biotechnol., 116, 113-124.
26. Silberstein,Z. and Cohen,A. (1987) Synthesis of linear multimers of OriC and pBR322 derivatives in Escherichia coli K-12: Role of recombination and replication functions. J. Bacteriol., 169, 3131-3137.
27. Miller,J.H. (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, NY.
28. Macian,F., Perez-Roger,I. and Armengod,M.E. (1994) An improved vector system for constructing transcriptional lacZ fusions: Analysis of regulation of the dnaA, dnaN, recF and gyrB genes of Escherichia coli. Gene, 145, 17-24.
29. Martinez-Perez,O., Moreno-Ruiz,E., Floriano,B. and Santero,E. (2004) Regulation of tetralin biodegradation and identification of genes essential for expression of thn operons. J. Bacteriol., 186, 6101-6109.
30. Amann,E. and Brosius,J. (1985) 'ATG vectors' for regulated high-level expression of cloned genes in Escherichia coli. Gene, 40, 183-190.
31. Kessler,B., de Lorenzo,V. and Timmis,K.N. (1993) Identification of a cis-acting sequence within the Pm promoter of the TOL plasmid which confers XylS-mediated responsiveness to substituted benzoates. J. Mol. Biol., 230, 699-703.
32. Winther-Larsen,H.C., Blatny,J.M., Valand,B., Brautaset,T. and Valla,S. (2000) Pm promoter expression mutants and their use in broad-host-range RK2 plasmid vectors. Metab. Eng., 2, 92-103.
33. Lutz,R. and Bujard,H. (1997) Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res., 25, 1203-1210.
34. Makrides,S.C. (1996) Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev., 60, 512-538.
35. Hanahan,D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol., 166, 557-580.
36. de Lorenzo,V., Fernandez,S., Herrero,M., Jakubzik,U. and Timmis,K.N. (1993) Engineering of alkyl- and haloaromatic-responsive gene expression with mini-transposons containing regulated promoters of biodegradative pathways of Pseudomonas. Gene, 130, 41-46.
37. Cases,I., Pérez-Martín,J. and de Lorenzo,V. (1996) Involvement of 54 in exponential silencing of the Pseudomonas putida TOL plasmid Pu promoter. Mol. Microbiol., 19, 7-17.