Improved expression systems for regulated expression in Salmonella infecting eukaryotic cells

PLoS ONE

Volumn 6, Issue 8, 2011, Pages

  Medina, Carlos ^a   Camacho, Eva María ^a   Flores, Amando ^a   Mesa Pereira, Beatriz ^a   Santero, Eduardo ^a  

^a UNIVERSIDAD PABLO DE OLAVIDE   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; COLICIN E3; EPITOPE; HYBRID PROTEIN; PROTEIN SSPH2; SIGNAL PEPTIDE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR NASF; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; COLICIN;

ANIMAL CELL; ARTICLE; BETA GALACTOSIDASE GENE; CELL VIABILITY; CONTROLLED STUDY; CYTOPLASM; CYTOSOL; EUKARYOTIC CELL; GENE DOSAGE; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; GENE LOCUS; GENETIC TRANSCRIPTION; GENOME; HELA CELL; HOST PATHOGEN INTERACTION; MOLECULAR CLONING; NASR GENE; NONHUMAN; PATHOGENESIS; PATHOGENICITY ISLAND; PLASMID; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN SYNTHESIS; PROTEIN TRANSPORT; REGULATOR GENE; SALMONELLA; SALMONELLOSIS; TYPE III SECRETION SYSTEM; XYLS2 GENE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BIOSYNTHESIS; GENE VECTOR; GENETICS; METABOLISM; SECRETION;

ANIMALIA; BACTERIA (MICROORGANISMS); EUKARYOTA; SALMONELLA;

BACTERIAL PROTEINS; CHROMOSOMES, BACTERIAL; CLONING, MOLECULAR; COLICINS; CYTOPLASM; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENETIC VECTORS; PLASMIDS; PROMOTER REGIONS, GENETIC; SALMONELLA;

EID: 79960951664     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0023055     Document Type: Article

References (39)

1. Bakke I, Berg L, Aune TE, Brautaset T, Sletta H, et al. (2009) Random mutagenesis of the PM promoter as a powerful strategy for improvement of recombinant-gene expression. Appl Environ Microbiol 75: 2002-2011.
2. Anthony LC, Suzuki H, Filutowicz M, (2004) Tightly regulated vectors for the cloning and expression of toxic genes. J Microbiol Methods 58: 243-250.
3. Royo JL, Manyani H, Cebolla A, Santero E, (2005) A new generation of vectors with increased induction ratios by overimposing a second regulatory level by attenuation. Nucleic Acids Res 33: e169.
4. Aune TE, Bakke I, Drabløs F, Lale R, Brautaset T, et al. (2010) Directed evolution of the transcription factor XylS for development of improved expression systems. Microbial biotechnology 3: 38-47.
5. Dong H, Nilsson L, Kurland CG, (1995) Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction. J Bacteriol 177: 1497-1504.
6. Stragier P, Losick R, (1990) Cascades of sigma factors revisited. Mol Microbiol 4: 1801-1806.
7. Cebolla A, Royo JL, De Lorenzo V, Santero E, (2002) Improvement of recombinant protein yield by a combination of transcriptional amplification and stabilization of gene expression. Appl Environ Microbiol 68: 5034-5041.
8. Royo JL, Moreno-Ruiz E, Cebolla A, Santero E, (2005) Stable long-term indigo production by overexpression of dioxygenase genes using a chromosomal integrated cascade expression circuit. J Biotechnol 116: 113-124.
9. Cebolla A, Sousa C, de Lorenzo V, (2001) Rational design of a bacterial transcriptional cascade for amplifying gene expression capacity. Nucleic Acids Res 29: 759-766.
10. Lin JT, Stewart V, (1996) Nitrate and nitrite-mediated transcription antitermination control of nasF (nitrate assimilation) operon expression in Klebsiella pheumoniae M5al. J Mol Biol 256: 423-435.
11. Chai W, Stewart V, (1998) NasR, a novel RNA-binding protein, mediates nitrate-responsive transcription antitermination of the Klebsiella oxytoca M5al nasF operon leader in vitro. J Mol Biol 283: 339-351.
12. Chai W, Stewart V, (1999) RNA sequence requirements for NasR-mediated, nitrate-responsive transcription antitermination of the Klebsiella oxytoca M5al nasF operon leader. J Mol Biol 292: 203-216.
13. Wu SQ, Chai W, Lin JT, Stewart V, (1999) General nitrogen regulation of nitrate assimilation regulatory gene nasR expression in Klebsiella oxytoca M5al. J Bacteriol 181: 7274-7284.
14. Royo JL, Becker PD, Camacho EM, Cebolla A, Link C, et al. (2007) In vivo gene regulation in Salmonella spp. by a salicylate-dependent control circuit. Nat Methods 4: 937-942.
15. Weissmann G, (1991) Aspirin. Sci Am 264: 84-90.
16. Thone F, Schwanhausser B, Becker D, Ballmaier M, Bumann D, (2007) FACS-isolation of Salmonella-infected cells with defined bacterial load from mouse spleen. J Microbiol Methods 71: 220-224.
17. Vazquez-Torres A, Jones-Carson J, Baumler AJ, Falkow S, Valdivia R, et al. (1999) Extraintestinal dissemination of Salmonella by CD18-expressing phagocytes. Nature 401: 804-808.
18. Valdivia RH, Hromockyj AE, Monack D, Ramakrishnan L, Falkow S, (1996) Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions. Gene 173: 47-52.
19. Carlomagno MS, Riccio A, Bruni CB, (1985) Convergently functional, Rho-independent terminator in Salmonella typhimurium. J Bacteriol 163: 362-368.
20. Wendland M, Bumann D, (2002) Optimization of GFP levels for analyzing Salmonella gene expression during an infection. FEBS Lett 521: 105-108.
21. Segura I, Casadesus J, Ramos-Morales F, (2004) Use of mixed infections to study cell invasion and intracellular proliferation of Salmonella enterica in eukaryotic cell cultures. J Microbiol Methods 56: 83-91.
22. Frantz B, O'Neill EA, (1995) The effect of sodium salicylate and aspirin on NF-kappa B. Science 270: 2017-2019.
23. Miao EA, Miller SI, (2000) A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium. Proc Natl Acad Sci U S A 97: 7539-7544.
24. Panthel K, Meinel KM, Domenech VE, Retzbach H, Igwe EI, et al. (2005) Salmonella pathogenicity island 2-mediated overexpression of chimeric SspH2 proteins for simultaneous induction of antigen-specific CD4 and CD8 T cells. Infect Immun 73: 334-341.
25. Spaink H, Okker R, Wijffelman C, Pees E, Lugtenberg B, (1987) Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI. Plant Molecular Biology 9: 27-39.
26. Cascales E, Buchanan SK, Duche D, Kleanthous C, Lloubes R, et al. (2007) Colicin biology. Microbiol Mol Biol Rev 71: 158-229.
27. Diaz E, Munthali M, de Lorenzo V, Timmis KN, (1994) Universal barrier to lateral spread of specific genes among microorganisms. Mol Microbiol 13: 855-861.
28. Salis HM, Mirsky EA, Voigt CA, (2009) Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol 27: 946-950.
29. Fass E, Groisman EA, (2009) Control of Salmonella pathogenicity island-2 gene expression. Curr Opin Microbiol 12: 199-204.
30. Galan JE, Collmer A, (1999) Type III secretion machines: bacterial devices for protein delivery into host cells. Science 284: 1322-1328.
31. Charpentier X, Oswald E, (2004) Identification of the secretion and translocation domain of the enteropathogenic and enterohemorrhagic Escherichia coli effector Cif, using TEM-1 beta-lactamase as a new fluorescence-based reporter. J Bacteriol 186: 5486-5495.
32. Pawelek JM, Low KB, Bermudes D, (2003) Bacteria as tumour-targeting vectors. Lancet Oncol 4: 548-556.
33. Low KB, Ittensohn M, Le T, Platt J, Sodi S, et al. (1999) Lipid A mutant Salmonella with suppressed virulence and TNFalpha induction retain tumor-targeting in vivo. Nat Biotechnol 17: 37-41.
34. Kasinskas RW, Forbes NS, (2007) Salmonella typhimurium lacking ribose chemoreceptors localize in tumor quiescence and induce apoptosis. Cancer Res 67: 3201-3209.
35. Sambrook J, Russell DW, (2001) Molecular cloning. A laboratory manual A laboratory manual, N.Y. Cold Spring Harbor Laboratory Press.
36. Miller JH, (1972) Experiments in molecular genetics. A laboratory manual, N.Y. Cold Spring Harbor Laboratory.
37. Datsenko KA, Wanner BL, (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97: 6640-6645.
38. Beuzon CR, Meresse S, Unsworth KE, Ruiz-Albert J, Garvis S, et al. (2000) Salmonella maintains the integrity of its intracellular vacuole through the action of SifA. Embo J 19: 3235-3249.
39. Senatus PB, Li Y, Mandigo C, Nichols G, Moise G, et al. (2006) Restoration of p53 function for selective Fas-mediated apoptosis in human and rat glioma cells in vitro and in vivo by a p53 COOH-terminal peptide. Mol Cancer Ther 5: 20-28.