Novel, versatile, and tightly regulated expression system for escherichia coli strains

Applied and Environmental Microbiology

Volumn 76, Issue 15, 2010, Pages 5058-5066

  Choi, Young J ^a,b   Morel, Lyne ^a   François, Teffanie Le ^a   Bourque, Denis ^a   Lucie, Bourget ^a   Groleau, Denis ^a   Massie, Bernard ^a,c   Miguez, Carlos B ^a,b  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b MCGILL UNIVERSITY   (Canada)

^c UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CONTROL TARGET; E. COLI; EXPRESSION PROFILE; EXPRESSION SYSTEM; EXPRESSION VECTORS; FED-BATCH FERMENTATION; GENE SWITCH; HIGH YIELD; HOST STRAIN; PRODUCT YIELDS; PROTEIN-ENCODING GENES; REGULATED GENES; REGULATORY ELEMENTS; TARGET PROTEINS; TRANSCRIPTIONAL LEVELS; WATERSOLUBLE;

ESCHERICHIA COLI; FERMENTATION; FLOW CYTOMETRY; GENE ENCODING; PROTEINS; STRAIN;

TRANSCRIPTION;

BENZOIC ACID DERIVATIVE; CUMIC ACID; RECOMBINANT PROTEIN;

COLIFORM BACTERIUM; FLOW CYTOMETRY; GENE EXPRESSION; PROTEIN;

ARTICLE; BIOSYNTHESIS; DRUG EFFECT; ESCHERICHIA COLI; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC ENGINEERING; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PSEUDOMONAS; PSEUDOMONAS PUTIDA; TRANSCRIPTION INITIATION; DRUG EFFECTS; PROCEDURES;

BENZOATES; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; GENETIC VECTORS; PSEUDOMONAS; PSEUDOMONAS PUTIDA; RECOMBINANT PROTEINS; TRANSCRIPTIONAL ACTIVATION;

ESCHERICHIA COLI; PSEUDOMONAS;

EID: 77955588811     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00413-10     Document Type: Article

References (49)

1. Baneyx, F. 1999. Recombinant protein expression in Escherichia coli. Curr. Opin. Biotechnol. 10:411-421.
2. Bhandari, P., and J. Gowrishankar. 1997. An Escherichia coli host strain useful for efficient overproduction of cloned gene products with NaCl as the inducer. J. Bacteriol. 179:4403-4406.
3. Bujard, H., R. Gentz, M. Lanzer, D. Stüber, M. Müller, I. Ibrahimi, M. T. Häuptle, and B. Dobberstein. 1987. A T5 promoter-based transcriptiontranslation system for the analysis of proteins in vitro and in vivo. Methods Enzymol. 155:416-433.
4. Chatterjee, D. K., and D. Esposito. 2006. Enhanced soluble protein expression using two new fusion tags. Protein Expr. Purif. 46:122-129.
5. Chaves, A. C., F. G. C. Abath, J. L. Lima-Filho, J. M. S. Cabral, and N. Lucena-Silva. 1999. Studies on growth kinetics and plasmid stability of recombinant Escherichia coli expressing a Schistosoma mansoni antigen. Bioprocess Engin. 21:355-361.
6. Choi, Y. J., C. B. Mfguez, and B. H. Lee. 2004. Characterization and heterologous gene expression of a novel esterase from Lactobacillus casei CL96. Appl. Environ. Microbiol. 70:3213-3221.
7. mxaF) of Methylobacterium extorquem by applying regulatory elements of Pseudomonas pulida F1. Appl. Environ. Microbiol. 72:7723-7729.
8. q/Ptrp-lac plasmids and transposons that confer conditional phenotypes. Gene 123:17-24.
9. De Mey, M., J. Maertens, G. J. Lequeux, W. K. Soetaert, and E. J. Vandamme. 2007. Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering. BMC Biotechnol. 7:34.
10. Eaton, R. W. 1996. p-Cumate catabolic pathway in Pseudomonas putida F1: cloning and characterization of DNA carrying the cmt Operon. J. Bacteriol. 178:1351-1362.
11. Eaton, R. W. 1997. p-Cymene catabolic pathway in Pseudomonas putida F1: cloning and characterization of DNA encoding conversion of p-cymene to p-cumate. J. Bacteriol. 179:3171-3180.
12. Figge, J., C. Wright, C. J. Collins, T. M. Roberts, and D. M. Livingston. 1988. Stringent regulation of stably integrated chloramphenicol acetyl transferase genes by E. coli lac repressor in monkey cells. Cell 52:713-722.
13. Georgiern, G., and L. Segatori. 2005. Preparative expression of secreted proteins in bacteria: status report and future prospects. Curr. Opin. Biotechnol. 16:538-545.
14. Giacalone, M. J., A. M. Gentile, B. T. Lovitt, N. L. Berkley, C. W. Gunderson, and M. W. Suber. 2006. Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system. Biotechniques 3:355-366.
15. Glick, B. R. 1995. Metabolic load and heterologous gene expression. Biotechnol. Adv. 13:247-261.
16. Grant, S. G. N., J. Jessee, F. R. Bloom, and D. Hanahan. 1990. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restrict ion mutants. Proc. Natl. Acad. Sci. U. S. A. 87:4645-4649.
17. Gupta, J. C., M. Jaisani, G. Pandey, and K. J. Mukherjee. 1999. Enhancing recombinant protein yields in Escherichia coli using the T7 system under the control of heat inducible XPL promoter. J. Biotechnol. 68:125-134.
18. Hanahan, D. 1985. Techniques for transformation of Escherichia coli, p. 109-135. In D. M. Glover (ed.), DNA cloning: a practical approach, vol. 1. IRL Press, McLean, VA.
19. Hashemzadeh-Bonehi, L., F. Mehraein-Ghomi, C. Mitsopoulos, J. P. Jacob, E. S. Hennessy, and J. K. Broome-Smith. 1998. Importance of using lac rather than ara promoter vectors for modulating the levels of toxic gene products in Escherichia coli. Mol. Microbiol. 30:676-678.
20. Hung, M. N., Z. Xia, N. T. Hu, and B. H. Lee. 2001. Molecular and biochemical analysis of two β-galactosidases from Bifidobacterium infantis HL96. Appl. Environ. Microbiol. 67:4256-4263.
21. Jaeger, K. E., A. Steinbüchel, and D. Jendrossek. 1995. Substrate specificities of bacterial polyhydroxyalkanoate depolymerases and lipases: bacterial lipases hydrolyze poly(omega-hydroxyalkanoates). Appl. Environ. Microbiol. 61:3113-3118.
22. Jana, S., and J. K. Deb. 2005. Strategies for the production of heterologous proteins in Escherichia coli. Appl. Microbiol. Biotechnol. 67:289-298.
23. Jensen, K. F. 1993. The Escherichia coli K-12 "wild types" W3110 and MG 1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low/pyrE expression levels. J. Bacteriol. 175:3401-3407.
24. Kaur, P., S. Agarwal, and S. Datta. 2009. Delineating bacteriostatic and bacteriocidal targets in Mycobacteria using IPTG inducible antisense expression. PLoS One 4:1-15.
25. Keasling, J. D. 1999. Gene-expression tools for the metabolic engineering of bacteria. Trends Biotechnol. 17:452-459.
26. Keyes, W., and A. Mills. 2003. Inducible systems see the light. Trends Biotechnol. 21:53-55.
27. Knodler, L. A., E. O. Sekyere, T. S. Stewart, P. J. Schofleld, and M. R. Edwards. 1998. Cloning and expression of a prokaryotic enzyme, arginine deiminase, from a primitive eukaryote Giardia intestinalis. J. Biol. Chem. 273:4470-4477.
28. Kosinski, M. J., U. Rinas, and J. E. Bailey. 1992. Isopropyl-beta-D- thiogalactopyranoside influences the metabolism of E. coli. Appl. Microbiol. Biotechnol. 36:782-784.
29. Lee, S. Y. 1996. High cell-density culture of Escherichia coli. Trends Biotechnol. 14:98-105.
30. Losen, M., B. Frölich, M. Pohl, and J. Büchs. 2004. Effect of oxygen limitation and medium composition on Escherichia coli fermentation in shakeflask cultures. Biotechnol. Prog. 20:1062-1068.
31. Mullick, A., Y. Xu, R. Warren, M. Koutroumanis, C. Guilbault, S. Broussau, F. Malenfant, L. Bourget, L. Lamoureux, R. Lo, A. Caron, A. Pilotte, and B. Massie. 2006. The cumate gene-switch: a system for regulated expression in mammalian cells. BMC Biotechnol. 6:43.
32. Osborne, M. J., Z. Su, V. Sridaran, and F. Ni. 2003. Efficient expression of isotopically labeled peptides for high resolution NMR studies: application to the Cdc42/Rac binding domains of virulent kinases in Candida albicans. J. Biomol. NMR 26:317-326.
33. Otto, C. M., F. Niagro, X. Su, and C. A. Rawlings. 1995. Expression of recombinant tumor necrosis factor is toxic to Escherichia coli. Clin. Diagn. Lab. Immunol. 2:740-746.
34. Patkar, A., N. Vijayasankaran, D. W. Urry, and F. Srienc. 2002. Flow cy- tometry as a useful tool for process development: rapid evaluation of expression systems. J. Biotechnol. 93:217-229.
35. Qing, G., L. C. Ma, A. Khorchid, G. V. Swapna, T. K. Mal, M. M. Takayama, B. Xia, S. Phadtare, H. Ke, T. Acton, G. T. Montelione, M. Ikura, and M. Inouye. 2004. Cold-shock induced high-yield protein production in. Escherichia coli. Nat. Biotechnol. 22:826-827.
36. Riesenberg, D., and R. Guthke. 1999. High cell density cultivation of microorganisms. Appl. Microbiol. Biotechnol. 51:422-430.
37. Rossi, F. M., and H. M. Blau. 1998. Recent advances in inducible gene expression systems. Curr. Opin. Biotechnol. 9:451-456.
38. Schein, C. H., and M. H. M. Noteborn. 1988. Formation of soluble recombinant proteins in E. coli is favored by lower growth temperature. Biotechnology (NY) 6:291-294.
39. a.Sevastsyanovich, Y. R., S. N. Alfasi, and J. A. Cole. 2010. Sense and nonsense from a systems biology approach to microbial recombinant protein production. Biotechnol. Appl. Biochem. 55:9-28.
40. Siegele, D. A., and J. C. Hu. 1997. Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations. Proc. Natl. Acad. Sci. U. S. A. 94:8168-8172.
41. Sørensen, H. P., and K. K. Mortensen. 2005. Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli. Microb. Cell Fact. 4:1.
42. Staub, J. M., B. Garcia, J. Graves, P. T. J. Hajdukiewicz, P. Hunter, N. Nehra, V. Paradkar, M. Schlittler, J. B. Carroll, I. Spatola, D. Ward, Y. E. Guangning, and D. A. Russell. 2000. High-yield production of a human therapeutic protein in tobacco chloroplasts. Nat. Biotechnol. 18:333-338.
43. Studier, F. W., and B. Moffatt. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:113-130.
44. Tao, H., C. Bausch, C. Richmond, F. R. Blattner, and T. Conway. 1999. Functional genomics: expression analysis of Escherichia coli growing on minimal and rich media. J. Bacteriol. 181:6425-6440.
45. Thiry, M., and D. Cingolani. 2002. Optimizing scale-up fermentation processes. Trends Biotechnol. 20:103-105.
46. Thomas, J. G., and F. Baneyx. 1996. Protein misfolding and inclusion body formation in recombinant Escherichia coli cells overexpressing heat-shock proteins. J. Biol. Chem. 271:11141-11147.
47. Wang, Z. W., W. S. Law, and Y. P. Chao. 2004. Improvement of the thermoregulated T7 expression system by using the heat-sensitive lad. Biotechnol. Prog. 20:1352-1358.
48. Yogender, P., J. C. Gupta, and K. J. Mukherjee. 2001. Optimizing recombinant protein expression in the T7 system under the control of the proUp promoter. Biotechnol. Lett. 23:41-46.
49. Yoon, S. H., M. J. Han, S. Y. Lee, K. J. Jeong, and J. S. Yoo. 2003. Combined transcriptome and proteome analysis of Escherichia coli during high cell density culture. Biotechnol. Bioeng. 81:753-767.