Review. Elimination of viruses in plants: Twenty years of progress

Spanish Journal of Agricultural Research

Volumn 11, Issue 1, 2013, Pages 173-188

  Panattoni, A ^a   Luvisi, A ^a   Triolo, E ^a  

^a UNIVERSITY OF PISA   (Italy)

Author keywords

Bibliographic research; Chemotherapy; Plant virus; Thermotherapy; Tissue culture

Indexed keywords

EID: 84874999207     PISSN: 1695971X     EISSN: None     Source Type: Journal    
DOI: 10.5424/sjar/2013111-3201     Document Type: Article

References (203)

1. Ahiabu RKA, Danso KE, Klu GYP, Bieler P, Msikita W, 1997. In vitro Cassava research capability at the Biotechnology and Nuclear Agriculture Research Institute (BNARI)Ghana: potentials and constraints. Afr J Root Tuber Crops 2: 209-212.
2. Arif M, Ibrahim M, Ahmad A, Hassan S, 2005. Elimination of Citrus tristeza closterovirus from citrus bud-wood through thermotherapy. Pak J Bot 37: 423-430.
3. Ayabe M, Sumi S, 2001. A novel and efficient tissue culture method-"Stem-disc dome culture"-for producing virus-free garlic (Allium sativum L.). Plant Cell Report 20: 503-507.
4. Balamuralikrishnan M, Sabithia D, Ganapathy T, Viswanathan R, 2003. Impact of serial thermotherapy on Sugarcane mosaic virus titre and regeneration in sugarcane. Arch Phytopal PFL 36: 173-178.
5. Barba M, 2001. Miglioramento sanitario del germoplasma di carciofo. L'Informatore Agrario 20: 35-37.
6. Bertaccini A, Botti S, Tabanelli D, Dradi G, Fogher C, Previati A, Re F, 2004. Micropropagation and establishment of mite-borne virus free garlic (Allium sativum). Acta Hort 631: 201-206.
7. Bertamini M, Muthuchelian K, Nedunchezhian N, 2004. Effect of grapevine leafroll on the photosynthesis of field grown grapevine plants (Vitis vinifera L. cv. Lagrein). J Phytopathol 152: 145-152.
8. Bhardwaj SV, Rai SJ, Thakur PD, Handa A, 1998. Meristem tip culture and heat therapy for production of Apple mosaic virus free plants in India. Acta Hort 472: 65-68.
9. Borroto-Fernández EG, Sommerbauer T, Popwich E, Schartl A, Laimer M, 2009. Somatic embryogenesis from anthers of the autochtonous Vitis vinifera cv. Domina leads to Arabis mosaic virus-free plants. Eur J Plant Pathol 124: 171-174.
10. Bruna A, 1997. Effect of thermotherapy and meristem-tip culture on production of virus-free garlic in Chile. Acta Hort 433: 631-634.
11. th Int Council of Virus and Virus-like of Grapevine. Adelaide (Austra-lia), March 12-17. pp: 165.
12. Carrington JC, Kasschau KD, Johansen LK, 2001. Activation and suppression of RNA silencing by plant viruses. Virology 281:1-5.
13. Cheema SS, Lore JS, Gosal SS, 1999. Management of citrus ringspot disease through thermotherapy. Ind Phytopath 52: 354-356.
14. Chellapan P, Vanitharani R, Ogbe F, Fauquet CM, 2005. Effects of temperature on geminivirus-induced RNA silencing in plants. Plant Physiol 138:1828-1841.
15. Chen WQ, Sherwood JL, 1991. Evaluation of tip culture, thermotherapy and chemotherapy for elimination of Peanut mottle virus from Arachis hypogea. J Phytopathol 132: 230-236.
16. Chen TH, Lu YT, 2000. Application of ribavirin in tissue culture of green bamboo (Bambusa oldhamii Munro) for elimination of Bamboo mosaic virus. Plant Prot Bull 42: 159-168.
17. Chen JZ, Li Q, 2001. Advances of research on elimination and detection of viruses in Prunus species. J Beijing Forestry 23: 71-74.
18. Chen Z, Wang X, Song B, Wang H, Bhadury PS, Yan K, Zhang H, Yang S, Jin L, Hu D, Xue W, Zeng S, Wang J, 2008. Synthesis and antiviral activities of novel chiral cyanoacrylate derivatives with (E) configuration. Bioorg Med Chem 16: 3076-3083.
19. Cieslinska M, 2002. Elimination of Apple chlorotic leaf spot virus (ACLSV) from pear by in vitro thermotherapy and chemotherapy. Acta Hort 596: 481-484.
20. Cieslinska M, 2003. Elimination of Strawberry mottle virus (SMoV) from Fragaria virginiana UC-11 indicator plants by thermotherapy and chemotherapy. Phytopathol Pol 30: 51-59.
21. Cieslinska M, Zawadzka B, 1999. Preliminary results of investigation on elimination of viruses from apple, pear, and raspberry using thermotherapy and chemotherapy in vitro. Phytopathol Pol 17: 41-48.
22. Conci VC, Perotto MC, Cafrune E, Lunello P, 2005. Program for intensive production of virus free garlic plants. Acta Hort 688: 195-200.
23. Conrad PL, 1991. Potato virus S-free plants obtained using antiviral compounds and nodal segment culture of potato. Am Potato J 68: 507-513.
24. Cooper VC, Walker DGA, 1978. Thermal inactivation of cherry leaf roll virus in tissue culture of Nicotiana rustica raised from seeds and meristem tips. Ann Appl Biol 88: 273-326.
25. Crotty S, Maag D, Cameron CE, Andino R, 2001. RNA virus error catastrophe: direct molecular test by using ribavirin. P Natl Acad Sci USA 12: 6895-6900.
26. D'Anna F, Panattoni A, Triolo E, 2006. Emerging antiviral drugs in plant chemotherapy. J Plant Pathol 88: 39.
27. Dalmay T, Horsefield R, Braunstein TH, Baulcombe DC, 2001. SD3 encodes an RNA helicase required for posttranscriptional gene silencing in Arabidopsis. EMBO J 20: 2069-2077.
28. De Clercq E, 2002. Highlights in the development of new antiviral agents. Mini-Rev Med Che 2: 163-175.
29. De Clercq E, 2005. Antiviral drug discovery and development: where chemistry meets with biomedicine. Antivir Res 67: 66-75.
30. D'Onghia AM, Carimi F, De Pasquale F, Djelouah K, Martelli GP, 2001. Elimination of Citrus psorosis virus by somatic embryogenesis from stigma and style cultures. Plant Pathol 50: 266-269.
31. Dunbar KB, Pinnow DL, Morris JB, Pittman N, 1993. Virus elimination from interspecific Arachis hybrids. Plant Dis 77: 517-520.
32. Dziedzic E, 2008. Elimination of Prunus necrotic ring spot (PNRSV) from plum "Earliblue" shoot through thermotherapy in vitro. J Fruit Ornam Plant Res 16: 101-109.
33. Ebi M, Kasai N, Masuda K, 2000. Small inflorescence bulbils are best for micropropagation and virus elimination in garlic. HortSci 35:735-737.
34. El-Amin SM, Valkonen JPT, Bremer R, Pehu E, 1994. Elimination of viruses and hypersensitivity to Potato virus Y (PVY) in an important Sudanese potato stock (Zalinge). Am Potato J 71: 267-272.
35. Faccioli G, 2001. Control of potato viruses using meristem and stem-cuttings cultures, thermotherapy and chemotherapy. In: Virus and virus-like diseases of potato and production of seed-potatoes (Loebenstein G, Berger PH, Brunt AA, Lawson RH, eds). Kluwer Acad Publ. Dordrecht (Netherlands), pp: 365-390.
36. Faccioli G, Colombarini A, 1991. PVS and PVM elimination by meristem culture, chemoand thermotherapy. Phytoparasitica 19: 3.
37. Faccioli G, Colombarini A, 1996. Correlation of Potato Virus S and Virus M contents of potato meristem tips with the percentage of virus-free plantlets produced in vitro. Potato Res 39: 129-140.
38. Faccioli G, Zoffoli R, 1998. Fast elimination of Potato virus X (PVX) and Potato virus S (PVS) from virus-infected potato stem-cuttings by chemotherapy. Phytopath Medit 37: 9-12.
39. Fajardo TVM, Torres AC, Buso JA, Ávila AC, Resende RO, 2002. Produçao e qualidade de bulbos de alho livre de suas principais viroses. Summa Phytopathologica 28: 207-2010.
40. Fang Y, Dhital SP, Li K, Khu D, Kim H, Song Y, Lim H, 2005. Utilization of single nodal cuttings and therapies for eradicating double-infected potato virus (PLRV, PVY) from in vitro plantlets of potato (Solanum tuberosum). J K Soc Hort Sci 48: 119-125.
41. Fitch MMM, Lehrer AT, Komor E, Moore PH, 2001. Elimination of Sugarcane yellow leaf virus from infected sugarcane plants by meristem tip culture visualized by tissue blot immunoassay. Plant Pathol 50:676-680.
42. Fletcher PJ, Fletcher JD, Lewthwaite SL 1998. In vitro elimination of onion yellow dwarf and shallot latent viruses in shallot (Allium cepa var. ascalonicum L.). New Zeal J Crop Hort 26: 23-26.
43. Fletcher PJ, Fletcher JD, 2001. In vitro virus elimination in three Andean root crops: oca (Oxalis tuberosa), ulluco (Ullucus tuberosus) and arracacha (Arracacia xanthorrhiza). New Zeal J Crop Hort 29: 23-27.
44. Fraga M, Alonso M, Borja M, 2004. Shoot regeneration rates of perennial phlox are dependant on cultivar and explants type. HortSci 39: 1373-1377.
45. Franchetti P, Cappellacci L, Grifantini M, 1996. IMP dehydrogenase as a target of antitumoral and chemotherapy. Il Farmaco 51: 457-469.
46. Freitas AJ, Rezende JAM, 1998. Odontoglossum ringspot virus-free Cymbidium obtained through meristem tip chemotherapy. Fitopatol Bras 23: 158-160.
47. Freitas AJ, Novacs QS, Betti JA, Rezende JAM, Kuniyuki H, Yuki VA, 2004. Identity of Apple stem pitting virus in Sao Paulo, Brazil, confirmed by RT-PCR. Fitopatol Bras 29: 691.
48. Gabova R, 1995. Chemotherapy treatment of Prunus spp. genotypes infected by plum pox virus. Rasteniev Dni-N 32: 16-18.
49. Gambino G, Bondaz J, Gribaudo I, 2006. Detection and elimination of viruses in callus, somatic embryos and regenerated plantlets of grapevine. Eur J Plant Pathol 114: 397-404.
50. Gambino G, Vallania R, Gribaudo I, 2010. In situ localization of Grapevine fanleaf virus and phloem-restricted viruses in embryogenic callus of Vitis vinifera. Eur J Plant Pathol 127: 557-570.
51. Gella R, Errea P, 1998. Application of in vitro therapy for Ilarvirus elimination in three Prunus species. J Phytopathol 146: 445-449.
52. Ghosh DK, Ahlawat YS, Gupta MD, 1997. Production of virus free garlic (Allium sativum) plants by thermotherapy and meristem tip culture. Ind J Agr Sci 67: 391-393.
53. Golino DA, Sim ST, Lee J., Rowhani A, 2007. Elimination of rose mosaic viruses using microshoot tip tissue culture. Acta Hort 751: 237-240.
54. Goussard PG, Wiid J, Kasdorf GGF, 1991. The effectiveness of in vitro somatic embryogenesis in eliminating fanleaf virus and leafroll associated viruses from grapevines. S Afr J Enol Vitic 12: 77-81.
55. Goussard PG, Wiid J, 1992. The elimination of fanleaf virus virus from grapevine using in vitro somatic embryogenesis combined with heat therapy. S Afr J Enol Vitic 13: 81-83.
56. Green SK, Luo CY, Wu SF, 1992. Elimination of leafcurl virus of sweet potato by meristem tip culture, heat and ribavirin. Plant Prot Bull Taipei 34: 1-7.
57. Gribaudo I, Lisa A, Lenzi R, Mannini F, 1999. Comportamento in vitro di un biotipo di Vitis vinifera cv. Albarola affetto da virosi (GLRaV 1 e 3, GVA) e risanato. Riv Vitic Enol 2: 3-7.
58. Gribaudo I, Gambino G, Cuozzo D, Mannini F, 2006. Attempts to eliminate Grapevine rupestris stem pittingassociated virus from grapevine clones. J Plant Pathol 88: 293-299.
59. Grout BWW, 1990. Meristem-tip culture for propagation and virus elimination. In: Plant cell culture protocols (Hall RD, ed). Humana Press Inc. Totowa (USA). pp: 115-125.
60. Gubareva LV, 2004. Molecular mechanisms of influenza virus resistance to neuraminidase inhbitors. Virus Res 103: 199-203.
61. Guidoni S, Mannini F, Ferrandino A, Argamante N, Di Stefano R, 1997. The effect of grapevine leafroll and rugose wood sanitation on agronomic performance and berry and leaf phenolic content of a Nebbiolo clone (Vitis vinifera). Am J Enol Viticult 48: 438-442.
62. Guta IC, Buciumeanu EC, Gheorghe RN, Teodorescu A, 2010. Solutions to eliminate Grapevine leafroll associated virus serotype 1+3 from V. vinifera L. cv. Ranai Magaraci. Rom Biotechnol Lett 15: 72-78.
63. th Meeting Int Council for the study of Virus and Virus-like Diseases of the Grapevine. Montreux (Switzerland), September 5-10. pp: 172.
64. Helliot B, Panis B, Poumay Y, Swennen R, Lepoivre P, Frison E, 2002. Cryopreservation for the elimination of Cucumber mosaic and Banana streak viruses from banana (Musa spp.). Plant Cell Rep 20: 1117-1122.
65. Helliot B, Panis B, Hernández R, Swennen R, Lepoivre P, Frison E, 2004. Development of in vitro techniques for the elimination of Cucumber mosaic virus from banana (Musa spp.). In: Banana improvement. Cellular, molecular biology, and induced mutations (Mohan JS, Swennen R, eds). Sci Publ Inc. Enfield (USA). pp: 183-191.
66. Hollings M, 1965. Disease control through virus-free stock. Ann Rev Phytopath 3: 367-396.
67. Horackova V, 1998. Potato virus S elimination by chemotherapy in vitro using ribavirin. Rostlinna Vyroba 44: 539-544.
68. Horackova V, Konrad J, Krpalkova A, 1999. Methods of production of virus-free material in maintenance breeding of potato variety Kerkovske rohlicky. Czech J Genet Plant 35: 25-28.
69. Huffman JH, Sidweell RW, Khare GP, Witkowski JT, Allen LB, Robins RK, 1973. In vitro effect of 1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide (Virazole, ICN 1229) on deoxyribonucleic acid and ribonucleic acid viruses. Antimicrob Agents Ch 3: 235-241.
70. Iwanami T, Ieki H, 1994. Elimination of citrus latter virus from shoots of potted citrus plants by ribavirin. J Phytopathol Soc Japan 60: 595-599.
71. James D, Trytten PA, Mackenzie DJ, Towers GHN, French CJ, 1997. Elimination of apple stem grooving virus by chemotherapy and development of an immunocapture RTPCR for rapid sensitive screening. Ann Appl Biol 131: 459-470.
72. Janeckova M, 1993. Elimination of a virus complex (PPV, PNRSV, PDV) from plum varieties using a combination of in vivo and in vitro cultures. Vedecke Prace Ovocnrske 13: 51-64.
73. Jayaram HN, Grusch M, Cooney DA, Krupitza G, 1999. Consequences of IMP dehydrogenase inhibition and its relationship to cancer and apoptosis. Curr Med Chem 6: 561-574.
74. Jayasinghe U, Salazar LF, 1997. Virus eradication: tissue culture of meristem, thermotherapy and chemotherapy. In: Techniques in plant virology (Jayasinghe U, Salazar LF, eds). International Potato Center. La Molina (Peru). pp: 1-8.
75. Jeeva ML, Balakrishnan S, Edison S, Rajmohan K, 2004. Meristem culture and thermotherapy in the management of Sweet potato feathery mottle virus (SPFMV). J Root Crops 30: 135-142.
76. Jiang L, Zhang MT, Chen ZX, Ma GH, 2005. Elimination of CymMv from tissue cultures of Cymbidium sinense with chemotherapy. Acta Hortic Sinica 32: 1056-1060.
77. Kabir AH, Pal SP, Sarker KK, Sharmin SA, Alam MF, 2010. Virus elimination and pathogen-free plantlets regeneration in Cucurbita pepo L. Arch Phytopathol PFL 43: 527-537.
78. Karesova R, Janeckova M, Paprstein F, 2002. Elimination of Raspberry bushy dwarf virus from raspberry cultivar 'Gatineau'. Acta Hort 585: 359-362.
79. Kassanis B, 1949. Potato tuber freed from Leaf roll virus by heat. Nature 164: 881.
80. Kassanis B, 1957. Some effects of varying temperature on the quality and quantity of Tobacco mosaic virus in infected plants. Virology 4: 187-199.
81. Kenganal MY, Amudai JA, Patil FS, Kulikarni UG, 2008. Feasibility of meristem culture for management of Banana streak virus (BSV) through micropropagation. Res Crop 9: 605-609.
82. Kim JY, Lee SY, Kim HR, Choi JK, 1994. Effect of virazole on elimination of viruses (LSV and CMV) from infected lily plants. RDA J Agric Sci Crop Protect 36: 370-374.
83. Kim HS, Jeon JH, Choi KH, Joung YH, Park SW, Joung H, 1996. Elimination of PVS (Potato virus S) by thermotherapy and chemotherapy in potato tissue culture. J Korean Soc Hortic Sci 37: 533-536.
84. Knapp E, Hanzer V, Weiss HH, Da Camara Machado A, Weiss B, Wang Q, Katinger H, Da Camara Machado ML, 1995. New aspects of virus elimination in fruit trees. Acta Hort 386: 409-418.
85. Kolbanova EV, Kukharchik NV, Barai VN, Zinchenko AI, 2004. Production of black currant free from raspberry ringspot virus using virazole in in vitro culture. Vestsi Natsyyanal nai Akademii Navuk Belarusi Seryya Biyalagichnykh Navuk 2: 90-93.
86. Komar V, Vigne E, Demangeat G, Fuchs M, 2007. Beneficial effect of selective virus elimination on the performance of Vitis vinifera cv. Chardonnay. Am J Enol Vitic 58: 202-210.
87. Koruza B, Jelaska S, 1993. Influence of meristem culture and virus elimination on phenotypical modifications of grapevine (Vitis vinifera L), cv. Refosk. Vitis 32: 59-60.
88. Koubouris GC, Maliogka VI, Efthimiou K, Katis NI, Vasilakakis MD, 2007. Elimination of Plum pox virus through in vitro thermotherapy and shoot tip culture compared to conventional heat treatment in apricot cultivar Bebecou. J Gen Plant Pathol 73: 370-373.
89. Kryszczuk A, 1999. Heat therapy and meristem tip culturesone of the methods of producing virus-free potato plants. Biuletyn Instytutu Hodowli I Aklimatyzacji Roslin 212: 151-157.
90. Kumar S, Khan MS, Raj SK, Sharma AK, 2009. Elimination of mixed infection of Cucumber mosaic and Tomato aspermy virus from Chrysanthemum morifolium Ramat. cv. Pooja by shoot meristem culture. Sci Hortic 119: 108-112.
91. Kuniyuki H, Betti JA, Costa AS, 1994. Termoterapia prolongada dificulta a obtençao de material propagativo livre de virus de videira. Fitopatol Bras 19: 209-213.
92. Laimer M, Hanzer V, Kriston V, Toth EK, Mendonca D, Kirilla Z, Balla I, 2006. Elimination and detection of pathogens from tissue cultures of Prunus sp. Acta Hort 725: 319-323.
93. Le CL, Pelet CLLF, Perko J, 1991. Assainissement de l'échalote (Allium ascalonicum L.): thermothèrapie et culture de méristèmes. Revue Suisse Vitic Arboric Hortic 23: 329-332.
94. Leonhardt W, Wawrosch C, Auer A, Kopp B, 1998. Monitoring of virus diseases in Austrian grapevine varieties and virus elimination using in vitro thermotherapy. Plant Cell Tiss Org 52: 71-74.
95. Lim ST, Wong SM, Goh CJ, 1993. Elimination of cymbidium mosaic virus and odontoglossum ringspot virus from orchids by meristem culture and thin section culture with chemotherapy. Ann Appl Biol 122: 289-297.
96. Ling J, Mingtao Z, Zexiong C, Guohua M, 2003. Eradication of CymMv from tissue cultures of Cymbidium sinense with chemotherapy. Acta Hortic Sin 2: 1056-1060.
97. Ling KS, 2010. Effectiveness of chemoand thermotherapeutic treatments on Pepino mosaic virus in tomato seed. Plant Dis 94: 325-328.
98. Li YW, Xu C, Chen JS, 2002. Establishment of virus-free taro (Colocasia esculenta cv Fenghuayunaitou) by meristemtip culture combined with thermotherapy. Pak J Plant Pathol 1: 40-43.
99. Loi JS, Lee SM, Lam Chan LT, Fan S, Yiang WH, 1991. Elimination of orchid viruses in Dendrobium Sonia using virazole. Singapore J Primary Indus 19: 16-22.
100. López-Delgado H, Mora-Herrera ME, Zavaleta-Mancera HA, Cadena-Hinojosa M, Scott IM, 2004. Salicylic acid enhances heat tolerance and Potato virus X (PVX) elimination during thermotherapy of potato microplants. Am J Potato Res 81: 171-176.
101. Luvisi A, Rinaldelli E, Panattoni A, Triolo E, 2012a. Membrane transport of antiviral drugs in plants: an electrophysiological study in grapevine explants infected by Grapevine leafroll associated virus 1. Acta Physiol Plant 34: 2115-2123.
102. Luvisi A, Panattoni A, Triolo E, 2012b. Eradication trials of Tobacco mosaic virus using chemical drugs. Acta Virol 56: 155-158.
103. Mahmoud SYM, Hosseny MH, Abdel-Ghaffar MH, 2009. Evaluation of some therapies to eliminate Potato Y potyvirus from potato plants. Int J Virol 5: 64-76.
104. Malaurie B, Trouslot MF, Berthaud J, Bouselem M, Pinel A, Duberm J, 1998. Medium-term and long-term in vitro conservation and safe international exchange of yam (Discorea spp.) germoplasm. Electron J Biotechn 1: 1-8.
105. Mangal M, Bhardwaj SV, Mangal AK, Kaur R, 2002. Production of Carnation latent virus-free stock of carnations. Phytomorphology 52: 1-6.
106. Manganaris GA, Economous AS, Boubourakas I, Katis N, 2003a. Production of virus-free plant propagation material from infected nectarine trees. Acta Hort 616: 501-505.
107. Manganaris GA, Economou AS, Bouborakas IN, Katis NI, 2003b. Elimination of PPV and PNRSV through thermotherapy and meristem-tip culture in nectarine. Plant Cell Rep 22: 195-200.
108. Mannini F, Argamante N, Credi R, 1996. Improvements in the quality of grapevine 'Nebbiolo' clones obtained by sanitation. Acta Hort 427: 106-111.
109. McClellan K, Perry CM, 2001. Oseltamivir. Drugs 61: 263-283.
110. th Int Council of Virus and Virus-like of Grapevine. Adelaide (Australia), March 12-17. pp: 174.
111. Mink GI, Wample R, Howell WE, 1998. Heat treatment of perennial plants to eliminate phytoplasmas, viruses and viroids while maintaining plant survival. In: Plant virus disease control (Hadidi A, Khetarpal RH, Koganezawa H, eds). APS Press, St Paul, (USA). pp: 332-345.
112. Mori K, Hosokawa D, 1977. Localization of viruses in apical meristem and production of virus-free plants by means of meristem and tissue culture. Acta Hort 78: 389-396.
113. Morris JB, Dunn S, Pinnow DL, Hopkins MS, Pittman RN, 1997. Meristem culture for virus elimination and peanut interspecific hybrid preservation. Crop Sci 37: 591-594.
114. Mourrain P, Beclin C, Elmayan T, Feuerbach F, Godon C, Morel J, Jouette D, Lacombe AM, Nikic S, Picault N, Remoue K, Sanial M, Vo TA, Vaucheret H, 2000. Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance. Cell 101: 533-542.
115. Nascimento LC, Pio Ribeiro G, Willadino L, Andrade GP, 2003. Stock indexing and Potato virus Y elimination from potato plants cultivated in vitro. Sci Agr 60: 525-530.
116. Navacchi O, Zuccherelli G, Zuccherelli S, 2005. In vitro multiplication of artichoke and virus elimination by thermotherapy and chemotherapy. Acta Hort 681: 397-401.
117. Nesi B, Trinchello D, Lazzereschi S, Grassotti A, Ruffoni B, 2009. Production of lily symptomless virus-free plants by shoot meristem tip culture and in vitro thermotherapy. Hortscience 44: 217-219.
118. Nukari A, Uosukainen M, Rokka VM, 2009. Cryopreservation techniques and their application in vegetatively propagated crop plants in Finland. Agr Food Sci 18: 117-128.
119. O'Herlihy EA, Croke JT, Cassells AC, 2003. Influence of in vitro factors on titre and elimination of model fruit tree viruses. Plant Cell Tiss Org 72: 33-42.
120. Pace B, Calabrese N, Bottalico G, Campanale A, Papanice MA, Frabco A, Finetti-Sialer M, Gallone P, Nigro F, Sumerano P, Vanadia S, Corrado A, Gallitelli D, 2008. Protocol for the sanitation and propagation under protected conditions of artichoke "Brindisino" clones. Giornate Fitopatologiche. Cervia (Italy), March 12-14. pp: 621-622
121. Panattoni A, D'Anna F, Triolo E, 2005. Differential antiviral activity of some IMPDH inhibitors. J Plant Pathol 87: 270-280.
122. th Meeting Int Council for the study of Virus and virus-like Diseases of the Grapevine. Stellenbosh (South Africa), April 3-7. pp: 139-140.
123. Panattoni A, D'Anna F, Triolo E, 2007a. Antiviral activity of tiazofurin and mycophenolic acid against Grapevine leafroll associated virus 3 in Vitis vinifera explants. Antivir Res 73: 206-211.
124. Panattoni A, D'Anna F, Cristani C, Triolo E, 2007b. Grapevine vitivirus A elimination in Vitis vinifera explants by antiviral drugs and thermotherapy. J Virol Methods 146: 129-135.
125. Panattoni A, Triolo E, 2010. Susceptibility of grapevine viruses to thermotherapy on in vitro collection of Kober 5BB. Sci Hortic 125: 63-67.
126. Paprstein F, Sedlak J, Polak J, Svobodova L, Hassan M, Bryxiova M, 2008. Results of in vitro thermotherapy of apple cultivars. Plant Cell Tiss Org 94: 347-352.
127. Park SW, Jeon JH, Kim HS, Joung H, 1994. Effects of antiviral chemical on elimination of Potato virus S in potato (Solanum tuberosum L.) shoot-tip culture. J Korean Soc Hortic Sci 35: 32-35.
128. Parmessur Y, Aljanabi S, Saumtally S, Dookun-Saumtally A, 2002. Sugarcane yellow leaf virus and Sugarcane yellow phytoplasma: elimination by tissue culture. Plant Pathol 51: 561-566.
129. Patena LF, Dolores LM, Bariring AL, Alcachupas AL, Laude NP, Barg E, Green SK, Barba RC, 2005. Improved technique for virus elimination in and production of certified planting materials of garlic (Allium sativum L). Acta Hort 694: 271-276.
130. Paunovic S, Jevremovic D, 2006. Elimination of viruses from the infected apple cv. Fuji Naga-Fu 2 by thermotherapy. Vocarstvo 40: 189-197.
131. Paunovic S, Ruzic D, Vujovic T, Milenkovic S, Jevremovic D, 2007. In vitro production of Plum pox virus-free plums by chemotherapy with ribavirin. Biotechnol Biotech Eq 21: 417-421.
132. Pennazio S, 1995. Effetto dell'energia termica sui virus delle piante superiori. Informatore Fitopatologico 9: 46-50.
133. Petrzik K, Svoboda P, 1997. Screening of Apple mosaic virus in hop cultivars in the Czech Republic by reverse transcription-polymerase chain reaction. Acta Virol 41: 101-103.
134. Polak J, Hauptmanova A, 2009. Preliminary results of in vivo thermotherapy of plum, apricot and peach cultivars artificially infected with PPV-M and PPV-D strains of Plum pox virus. Hort Sci 36: 92-96.
135. th Meeting Int Council for the Study of Virus and virus-like Diseases of the Grapevine. Bari (Italy), September 12-17. pp: 243.
136. Popescu CF, Visoiu E, Buciumeanu E, Teodorescu A, Gheorghe RN, Tanasescu C, Ciocirlan CN, 2010. From plant tissue culture to modern biotechnology at the National Research and Development Institute for Biotechnology in Horticulture Stefanesti: achievements and prospects. Romanian Biotechnol Lett 15(2): 78-87.
137. Postman JD, 1997. Blueberry scorch carlavirus eliminated from infected blueberry (Vaccinium corymbosum) by heat therapy and apical meristem culture. Plant Dis 81: 111.
138. Postman JD, Sugar D, 2002. Elimination of viruses from the USDA Pyrus germplams collection. Acta Hort 596: 529-530.
139. Pozzer L, Dusi AN, Silva JBC, Kitajma EW, 1994. Avaliaçao da taxa de reinfecçao de plantas de batata-doce livres de virus pelo "Sweet potato feathery mottle virus" em condiçoes de campo. Fitopatol Bras 19: 231-234.
140. Previati A, Benelli C, Da Re F, Ozudogru A, Lambardi M, 2008. Micropropagation and in vitro conservation of virusfree rose germoplasm. Propag Ornam Plants 8: 93-98.
141. Qu F, Ye X, Hou G, Sato G, Clemente TE, Morris TJ, 2005. RDR6 has a broad-spectrum but temperature-dependent antiviral defense role in Nicotiana benthamiana. J Virol 9: 15209-15217.
142. Quainoo AK, Wetten AC, Allainguillaume J, 2008. The effectiveness of somatic embryogenesis in eliminating the Cocoa swollen shoot virus from infected cocoa trees. J Virol Methods 149: 91-96.
143. Ram R, Verma N, Singh AK, Singh L, Hallan V, Zaidi AA, 2005. Indexing and production of virus-free chrysanthemums. Biol Plantarum 49: 149-152.
144. Ram R, Verma N, Singh AK, Zaidi AA, 2009. Virus-free chrysanthemums: production and quality management. Arch Phytopathol PFL 42: 940-949.
145. Ramgareeb S, Snyman SJ, Van Antwerpen T, Rutherford RS, 2010. Elimination of virus and rapid propagation of diseasefree sugarcane (Saccharum spp. cultivar NCo376) using apical meristem culture. Plant Cell Tiss Org 100: 175-181.
146. Ramírez-Malagón R, Pérez-Moreno L, Borodanenko A, Salinas-González GJ, Ochoa-Alejo N, 2006. Differential organ infection studies, potyvirus elimination, and field performance of virus-free garlic plants produced by tissue culture. Plant Cell Tiss Org 86:103-110.
147. Ramos CS, Zamora AB, 1999. Isolation of banana plantlets cv. señorita freed of Banana bract mosaic virus. Philipp Agric Sci 82: 25-34.
148. Refatti E, Carraro L, Osler R, Soligo S, 1999. Alcuni dati sul risanamento mediante calore di materiale di moltiplicazione per pomacee affetto da agenti virali. Rivista di Frutticoltura e di Ortofloricoltura 61: 91-95.
149. Rinaldelli E, Panattoni A, Luvisi A, Triolo E, 2012. Effect of mycophenolic acid on trans-plasma membrane electron transport and electric potential in virus-infected plant tissue. Plant Physiol Bioch 60:137-140.
150. Robert U, Zel J, Ravnikar M, 1998. Thermotherapy in virus elimination from garlic: influences on shoot multiplication from meristems and bulb formation in vitro. Sci Hortic 73: 193-202.
151. Ruitz MT, Voinnet O, Baulcombe DC, 1998. Initiation and maintenance of virus-induced gene silencing. Plant Cell 10: 937-946.
152. Salami SA, Ebadi A, Zamani Z, Habibi MK, 2009. Incidence of Grapevine fanleaf virus in Iran: a survey study and production of virus-free material using meristem culture and thermotherapy. Europ J Hort Sci 74: 42-46.
153. Saponari M, Bottalico G, Savino V, 1999. In vitro propagation of Prunus mahaleb and its sanitation from Prune dwarf virus. Adv Hort Sci 13: 56-60.
154. Saponari M, Bottalico G, Loconsole G, Mondelli G, Campanale A, Savino V, Martelli GP, 2007. Olive viruses and strategies for producing virus-free plants. Bulletin OILB/SROP 30: 9-217.
155. Schulze S, Kluge S, 1994. The mode of inhibition of TMVand PVX-induced RNA-dependent RNA polymerase by some antiphytoviral drugs. J Phytopathol 141: 77-85.
156. Sedivá J, Nova P, Laxa J, KaAka J, 2006. Micropropagation, detection and elimination of DMV in the Czech collection of Dahlia. Acta Hort 725: 495-498.
157. Sedlak J, Paprstein F, Svobodova V, Polak J, Hassan M, Bryxiova M, 2007. Preliminary results of in vitro thermotherapy of apple and pear. Vedecke Prace Ovocnarske 20: 73-79.
158. Senula A, Keller ERJ, Leseman DE, 2000. Elimination of viruses through meristem culture and thermotherapy for the establishment of an in vitro collection of garlic (Allium sativum L.). Acta Hortic 530: 121-128.
159. Sharma S, Singh B, Rani G, Zaidi AA, Hallan VK, Nagpal AK, Virk GS, 2008. In vitro production of Indian citrus ringspot virus (ICRSV) free Kinnow plants employing thermotherapy coupled with shoot tip grafting. Plant Cell Tiss Org 92: 85-92.
160. Shiboleth YM, Gal-On A, Koch M, Rabinowitch D, Salomon R, 2001. Molecular characterization of Onion yellow dwarf virus (OYDV) infecting garlic (Allium sativum L.) in Israel: thermotherapy inhibits virus elimination by meristem tip culture. Ann Appl Biol 138: 187-195.
161. Sidwell RW, Huffman JH, Share GP, Allen LB, Witkowski JT., Robins RK, 1972. Broad spectrum antiviral activity of virazole 1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide. Science 177: 705-706.
162. Singh BR, Dubey VK, Aminuddin, 2007. Inhibition of mosaic disease of gladiolus caused by Bean yellow mosaicand Cucumber mosaic viruses by virazole. Sci Hortic 114: 54-58.
163. Skiada FG, Grigoriadou K, Maliogka VI, Katis NI, Eleftheriou EP, 2009a. Elimination of Grapevine leafroll-associated virus 1 and Grapevine rupestris stem pitting-associated virus from grapevine cv. Agiorgitiko, and a micropropagation protocol for mass production of virus-free plantlets. J Plant Pathol 91: 177-184.
164. Skiada FG, Maligka VI, Eleftheriou EP, Katis NI, 2009b. Advances on the elimination of Grapevine rupestris stem pitting associated virus (GRSPaV) from Vitis vinifera explants. Proc XVI Meeting Int Council for the Study of Virus and Virus-like diseases of the Grapevine. Dijon (France), August 31-September 3. pp: 262-263.
165. Spiegel S, Frison EA, Converse RH, 1993. Recent development in therapy and virus-detection procedures for international movements of clonal plant germ plasm. Plant Dis 77: 176-1180.
166. Spiegel S, Stein A, Tam Y, 1995. In vitro thermotherapy of rosaceous fruit trees. Acta Hort 386: 419-420.
167. Stein A, Spiegel S, Faingersh G, Levy S, 1991. Responses of micropropagated peach cultivars to thermotherapy for the elimination of Prunus necrotic ringspot virus. Ann Appl Biol 119: 265-271.
168. Stein DS, Creticos CM, Jackson GG, 1987. Oral ribavirin treatment of influenza A and B. Antimicrob Agents Ch 31: 285-1287.
169. Streeter DG, Witkowski JT, Khare GP, Sidwell RW, Bauer RJ, Robins RK, Simon LN, 1973. Mechanism of action of 1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide (Ribavirin) a new broad-spectrum antiviral agent. Proc Natl Acad Sci 70: 1174-1178.
170. Szittya G, Silhavy D, Moinar A, Havelda Z, Lovas A, Lakatos L, Banfalvi Z, Burgyan J, 2003. Low temperature inhibits RNA silencing-mediated defence by the control of siRNA generation. EMBO J 22: 633-640.
171. Talacko L, Sedlak J, Paprestein F, 2007. Testing of apple and pear by quick diagnostic methods (PCR, RT-PCR, DASELISA) in the course of sanitation process: first results. Vedecke Prace Ovocnarske 20: 91-97.
172. Tan RR, Wang LP, Hong N, Wang GP, 2010. Enhanced efficiency of virus elimination following thermotherapy of shoot-tip cultures of pear. Plant Cell Tiss Org 101: 229-235.
173. Tomassoli L, Di Lernia G, Tiberini A, Chiota G, Catenaro E, Barba M, 2008. In vitro thermotherapy and shoot-tip culture to eliminate Caper latent virus in Capparis spinosa. Phytopathol Mediterr 47: 115-121.
174. Toussaint A, Kummert J, Maroquin C, Lebrun A, Roggemans J, 1993. Use of virazole to eradicate Odontoglossum ringspot virus from in vitro cultures of Cymbidium Sw. Plant Cell Tiss Org 32: 303-309.
175. Truskinov EV, Rogozina EV, 1997. Elimination of viruses from a potato clone collection by tissue culture. Russ J Plant Phys 44: 374-380.
176. Uchanski M, Skirvin RM, Norton MA, 2002. The use of in vitro thermotherapy to obtain Turnip mosaic virus-free horseradish plants. Acta Hort 631: 175-179.
177. Valero M, Ibanez A, Morte A, 2003. Effects of high vineyard temperatures on the Grapevine leafroll associated virus elimination from Vitis vinifera L. cv. Napoleon tissue cultures. Sci Hortic 97: 289-296.
178. Vance V, Vaucheret H, 2001. RNA silencing in plants-defense and counterdefense. Science 292: 2277-2280.
179. Verma N, Ram R, Zaidi AA, 2005. In vitro production of Prunus necrotic ringspot virus-free begonias trough chemoand thermotherapy. Sci Hort 103: 239-247.
180. th ISSCT. New Delhi (India), February 22-26. pp: 390-397.
181. Voinnet O, 2001. RNA silencing as a plant immune system against viruses. Trends Genet 17: 449-459.
182. Wang LP, Wang GP, Hong N, Tang RR, Deng XY, Zhang H, 2006. Effect of thermotherapy on elimination of Apple stem grooving virus and Apple chlorotic leaf spot virus for in vitro-cultured pear shoot tips. Hortscience 41: 729-732.
183. Wang LP, Hong N, Wang GP, Xu WX, Michelutti R, Wang AM, 2010. Distribution of Apple stem growing and Apple chlorotic leaf spot virus in infected in vitro shoots. Crop Prot 29: 1447-1451.
184. Wang K, Hu Y, Liu Y, Mi N, Fan Z, Liu Y, Wang Q, Design, 2010a. Synthesis and antiviral evaluation of phenanthrene-based tylophorine derivatives as potential antiviral agents. J Agric Food Chem 58(23): 12337-12342.
185. Wang K, Su B, Wang Z, Wu M, Li Z, Hu Y, Fan Z, Mi N, Wang Q, 2010b. Synthesis and antiviral activities of phenanthroindolizidine alkaloids and their derivatives. J Agric Food Chem 58 (5): 2703-2709.
186. Wang Q, Cuellar WJ, Rajamaki ML, Hirata Y, Valkonen JPT, 2008. Combined thermotherapy and cryotherapy for efficient virus elimination: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Mol Plant Pathol 9: 237-250.
187. Wang Q, Valkonen JPT, 2009. Improved recovery of cryotherapy-treated shoot tips following thermotherapy of in vitrogrown stock shoots of raspberry (Rubus idaeus L.). Cryoletters 30: 171-182.
188. Wang Q, Panis B, Engelmann F, Lambardi M, Valkonen JPT, 2009. Cryotherapy of shoot tips: a technique for pathogen elimination to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation. Ann Appl Biol 154: 351-363.
189. Wangai AW, Bock KR, 1996. Elimination of Ornithogalum mosaic virus from chincherinchee (Ornithogalum thyrsoides) by meristem tip culture, and field trials of reinfection. Plant Pathol 45:767-768.
190. Wasswa P, Alicai T, Mukasa SB, 2010. Optimisation of in vitro techniques for Cassava brown streak virus elimination from infected cassava clones. Afr Crop Sci J 18: 235-241.
191. Weiland CM, Cantos M, Troncoso A, Perez-Camacho F, 2004. Regeneration of virus-free plants by in vitro chemotherapy of GFLV (Grapevine fanleaf virus) infected explants of Vitis vinifera L. cv "Zalema". Acta Hortic 652: 463-466.
192. Xi Z, Zhang R, Yu Z, Ouyang D, 2006. The interaction between tylophorine B and TMV next term RNA. Bioorg Med Chem Lett 16(16): 4300-4304.
193. Xia Y, Fan Z, Yao J, Liao Q, Li W, Qua F, Peng L, 2006. Discovery of bitriazolyl compounds as novel antiviral candidates for combating the Tobacco mosaic virus. Bioorg Med Chem Lett 16: 2693-2698.
194. Xu PS, Niimi Y, 1999. Evaluation of virus free bulblets production by antiviral and/or heat treatment in in vitro scale cultures of Lilium longiflorum 'Georgia' and L.x 'Casablanca'. J Jpn Soc Hortic Sc 68: 640-647.
195. Xu PS, Niimi Y, Araki H, 2000. Production of virus free bulblets from callus induced from scale culture of Lilium longiflorum 'Georgia'. J Jpn Soc Hortic Sc 69: 97-102.
196. Xu WY, Chen ZY, Liao HX, 2004. Effects of sulphates on ribavirin inhibition to tomato virus disease. J Fujian Agric Forest 33: 162-165.
197. Yamaga H, Munakata T, 1991. Production of virus-free apple planting stock by meristem culture. Techn Bull Food Fertil Technol Cent 126: 10-17.
198. Yap ML, Lam-Chan LT, Yik CP, Kueh J, Lee SM, Loh HM, 1999. Elimination of viruses from commercial orchids. Singapore J Primary Indus 27: 11-19.
199. Youssef SA, Al-Dhaher MMA, Shalaby AA, 2009. Elimination of Grapevine fanleaf virus (GFLV) and Grapevine leaf roll-associated virus-1 (GLRaV-1) from infected grapevine plants using meristem tip culture. Int J Virol 5: 89-99.
200. Zhang ZH, Xiao M, Yang HY, Li H, Gao XY, Du GD, 2006. Evaluation and comparison on methods of virus elimination from the strawberry plants. J Fruit Sci 23: 720-723.
201. Zhao WG, Wang JG, Li ZM, Yang Z, 2006. Synthesis and antiviral activity against Tobacco mosaic virus and 3DQSAR of α-substituted-1,2,3-thiadiazoleacetamides. Bioorg Med Chem Lett 16: 6107-6111.
202. Zilkah S, Faingersh G, Rotbaum A, David I, Spiegel S, Tam Y, Rieger-Stein A, 2001. Field performance of in vitro propagated virus-free 'Hermosa'peach. Acta Hortic 560: 551-554.
203. Zilka S, Faingersh G, Rotbaum A, Malca N, Tam Y, Spiegel S, 2002. In vitro production of virus-free pear plants. Acta Hort 596: 477-479.