Elimination of viruses through meristem culture and thermotherapy for the establishment of an in vitro collection of garlic (ALLIUM SATIVUM)

Acta Horticulturae

Volumn 530, Issue , 2000, Pages 121-128

  Senula, A ^a   Keller, E R J ^a   Leseman, D E ^b  

^a LEIBNIZ INSTITUTE OF PLANT GENETICS AND CROP PLANT RESEARCH IPK   (Germany)

^b INSTITUTE FOR EPIDEMIOLOGY AND PATHOGEN DIAGNOSTICS   (Germany)

Author keywords

Apices; Bulbils; Garlic common latent virus; Leek yellow stripe virus; Mite borne filamentous virus; Onion yellow dwarf virus; Ribavirin; Shallot latent virus

Indexed keywords

ACARI; ALLIUM ASCALONICUM; ALLIUM CEPA; ALLIUM PORRUM; ALLIUM SATIVUM; GARLIC COMMON LATENT VIRUS; LEEK YELLOW STRIPE VIRUS; ONION YELLOW DWARF VIRUS; SHALLOT LATENT VIRUS;

EID: 33645614451     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/ActaHortic.2000.530.12     Document Type: Conference Paper

References (18)

1. Barg, E., Lesemann, D.-E., and Vetten, H.J., 1994. Identification, partial characterization, and distribution of viruses infecting Allium crops in south and southeast asia. Acta Hortic. 358:251-258.
2. Bhojwani, S. S., 1980. In vitro propagation of garlic by shoot proliferation. Sci. Hortic. 13:47-52.
3. Cassells, A., and Long, R.D., 1980. The regeneration of virus-free plants from cucumber mosaic virus-and potato virus Y-infected tobacco expiants cultured in the presence of virazole. Z. Naturforsch. 35c: 350-351.
4. Clark, M.F., and Adams, A.N., 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for detection of plant viruses. J. Gen. Virol. 34: 205-217.
5. Conci, V.C., and Nome, S.F., 1991. Virus free garlic (Allium sativum L.) plants obtained by thermotherapy and meristem tip culture. J. Phytopathology. 132: 186-192.
6. Dunstan, D.I., and Short, K.S., 1977. Improved growth of tissue cultures of the onion, Allium cepa. Physiol. Plant. 41: 70-72.
7. Kim, J.Y., Choi, S.T., Roh, M.S., and Ko, T.S., 1996. Production and detection of virus-free Lily plants by shoot tip culture and virazole treatment of bulbils. J. Kor. Soc. Hortic. Sci. 37: 64-69.
8. Milne, R.G., and Lesemann, D.-E., 1984. Immunosorbent electron microscopy in plant virus studies. Methods in Virology. 8: 85-101.
9. Moriconi, D. N., Conci, V.C., and Nome, S.F., 1990. Rapid multiplication of garlic (Allium sativum L.) in vitro. Phyton. 51: 145-151.
10. Murashige, T., and Skoog, F., 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15: 473-487.
11. Raj, S. K., Aminuddin, M. A., and Singh, B. P., 1991. Elimination of eggplant mottled crinkle virus using virazole in expiant cultures of Solanum melongena L. Indian J. Exp. Biol. 29: 594-595.
12. Szyndel, M. S., Kozera, B., and Misterek, E., 1994. The elimination of viruses from garlic (Allium sativum L.) plants by thermotherapy and meristem tip cultures. Acta Agro-botanica 47: 83-88.
13. Ucman, R., Zel, J., and Ravnikar, M., 1998. Thermotherapy in virus elimination from garlic: influences on shoot multiplication from meristems and bulb formation in vitro. Sci. Hortic. 73: 193-202.
14. Van Dijk, P., 1994. Virus diseases of Allium species and prospects for their control. Acta Hortic. 358: 299-306.
15. Verbeek, M., Van Dijk, P., and Van Well, P. M. A., 1995. Efficiency of eradication of four viruses from garlic (Allium sativum) by meristem-tip culture. European J. Plant Pathol. 101:231-239.
16. Vetten, H. J., Lesemann, D.-E., and Maiss, E., 1992. Serotype A and B isolates of bean common mosaic virus are two distinct potyviruses. Arch. Virol. Suppl. 5: 415-431.
17. Walkey, D. G. A., Webb, M. J., Bolland, C. J., and Miller, A., 1987. Production of virus-free garlic (Allium sativum L.) and shallot (A. ascalonicum L.) by meristem-tip culture. J. Hortic. Sci. 62: 211-220.
18. Walkey, D. G. A., 1990. Virus diseases. In: Onions and Allied Crops (Eds. Rabinowitch, H. D., and Brewster, J.L.). Vol. II: CRC Press, Inc, Boca Raton. Pp. 191-212.