Efficient production of active polyhydroxyalkanoate synthase in Escherichia coli by coexpression of molecular chaperones

Applied and Environmental Microbiology

Volumn 79, Issue 6, 2013, Pages 1948-1955

  Thomson, Nicholas M ^a   Saika, Azusa ^b   Ushimaru, Kazunori ^b   Sangiambut, Smith ^a   Tsuge, Takeharu ^b   Summers, David K ^c   Sivaniah, Easan ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CO-EXPRESSION; COEXPRESSED; EFFICIENT PRODUCTION; GROEL/GROES; MOLECULAR CHAPERONES; OVER-EXPRESSION; POLY-HYDROXYALKANOATE; POLYHYDROXYALKANOATE SYNTHASE; PRODUCTION OF; PURIFIED ENZYME; SOLUBLE FRACTION; SPECIFIC ACTIVITY; SYNTHASES;

ESCHERICHIA COLI; POLYMERS;

ENZYME ACTIVITY;

ACYLTRANSFERASE; BACTERIAL PROTEIN; CHAPERONIN; GROESL PROTEIN, BACTERIA; POLY(3-HYDROXYALKANOIC ACID) SYNTHASE; POLYHYDROXYALKANOATE SYNTHASE; RECOMBINANT PROTEIN;

COLIFORM BACTERIUM; ENZYME ACTIVITY; MOLECULAR ANALYSIS; POLYMER; PROTEIN;

ARTICLE; BIOSYNTHESIS; CUPRIAVIDUS NECATOR; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM;

ACYLTRANSFERASES; BACTERIAL PROTEINS; CHAPERONINS; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; GENE EXPRESSION; RECOMBINANT PROTEINS;

EID: 84874791440     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02881-12     Document Type: Article

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