Continuous-culture studies on the stability and expression of recombinant streptokinase in Escherichia coli

Bioprocess and Biosystems Engineering

Volumn 24, Issue 6, 2002, Pages 341-346

  Yazdani, S ^a   Mukherjee, K ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; PLASMIN; RECOMBINANT ENZYME; STREPTOKINASE;

ARTICLE; CELL GROWTH; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONTINUOUS CULTURE; CONTROLLED STUDY; CYTOPLASM; CYTOTOXICITY; DILUTION; ENZYME STABILITY; ESCHERICHIA COLI; HEAT TREATMENT; NONHUMAN; PLASMID; PRESSURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN SYNTHESIS;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0035723632     PISSN: 16157591     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004490100221     Document Type: Article

References (19)

1. Investigation of the instability of plasmids directing the expression of Met-prochymotrypsin in Escherichia coli
2. Plasmid-encoded protein: The principal factor in the 'metabolic burden' associated with recombinant bacteria
3. Metabolic load and heterologous gene expression
4. A perspective on the application of genetic engineering: Stability of recombinant plasmid
5. Effect of growth environment on the stability of a recombinant shuttle plasmid, pCPPS-31, in Escherichia coli
6. Effect of growth rate on stability and gene expression of recombinant plasmids during continuous and high cell density cultivation of Escherichia coli TG1
7. Plasmid maintenance in Escherichia coli recombinant cultures is dramatically, steadily and specifically influenced by features of the encoded proteins
8. Effect of the selection marker on the viability and plasmid stability of two human proteins with neurotrophic action expressed in Escherichia coli
9. Streptokinase: Cloning, expression, and excretion by Escherichia coli
10. Streptokinase mutations relieving Escherichia coli K-12 (prlA4) of detriments caused by the wild-type skc gene
11. Overexpression of streptokinase using a fed-batch strategy
12. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes
13. L promoter
14. Use of dinitrosalicylic acid reagent for determination of reducing sugars
15. Effect of glucose supply strategy on acetate accumulation, growth, and recombinant protein production by Escherichia coli BL21 (λDE3) and Escherichia coli JM109
16. Effect of growth rate on the production of Pseudomonas fluorescens lipase during the fed-batch cultivation of Escherichia coli
17. Kinetic study of instability of recombinant plasmid pPLc23trpA1 in E. coli using two-stage continuous-culture system
18. Effect of preinduction specific growth rate on recombinant alpha consensus interferon synthesis in Escherichia coli
19. The effects of plasmid content, transcription efficiency on the productivity of a cloned gene protein in Escherichia coli