Effective production and kinetic characterization of ultra-high-molecular- weight poly[(R)-3-hydroxybutyrate] in recombinant Escherichia coli

Polymer Degradation and Stability

Volumn 87, Issue 1, 2005, Pages 161-169

  Kahar, Prihardi ^a   Agus, Jumiarti ^a   Kikkawa, Yoshihiro ^b   Taguchi, Kazunori ^b   Doi, Yoshiharu ^a,b   Tsuge, Takeharu ^a  

^a JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^b RIKEN   (Japan)

Author keywords

Bioengineering; Biopolyester; Chain transfer reaction; High cell density culture; Molecular weight; Polyhydroxyalkanoate

Indexed keywords

BIOMEDICAL ENGINEERING; BIOSYNTHESIS; CELL CULTURE; DNA; ESCHERICHIA COLI; GENES; GLUCOSE; MOLECULAR WEIGHT; POLYESTERS; PROTEINS; PYROLYSIS;

BIOPOLYESTERS; CHAIN TRANSFER REACTION; HIGH CELL DENSITY CULTURE; POLYHYDROXYALKANOATE;

BIOPOLYMERS;

EID: 9444222531     PISSN: 01413910     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.polymdegradstab.2004.08.002     Document Type: Article

References (24)

1. Doi Y. In: Microbial Polyesters. New York: VCH Publisher; 1990.
2. Steinbüchel A. Perspectives for biotechnological production and utilization of biopolymers: metabolic engineering of polyhydroxy-alkanoate biosynthesis pathway as a successful example. Macromol Biosci 2001;1:1-24.
3. Akiyama M, Tsuge T, Doi Y. Environmental life cycle comparison of polyhydroxyalkanoates produced from renewable carbon resources by bacterial fermentation. Polym Degrad Stab 2003;80:183-94.
4. Kahar P, Tsuge T, Taguchi K, Doi Y. High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain. Polym Degrad Stab 2004;83:79-86.
5. Sudesh K, Abe H, Doi Y. Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters. Prog Polym Sci 2000;25:1503-55.
6. Lee SY, Lee KM, Chang HN, Steinbüchel A. Comparison of Escherichia coli strains for synthesis and accumulation of poly-(3-hydroxybutyric acid), and morphological changes. Biotechnol Bioeng 1994;44:1337-47.
7. Lee SY. Suppression of filamentation in recombinant Escherichia coli by amplified FtsZ activity. Biotechnol Lett 1994;1247-52.
8. Wang F, Lee SY. Production of poly(3-hydroxybutyrate) by fed-batch culture of filamentation-suppressed recombinant Escherichia coli. Appl Environ Microbiol 1997;63:4765-9.
9. Kusaka S, Abe H, Lee SY, Doi Y. Molecular mass of poly[(R)-3- hydroxybutyric acid] produced in a recombinant Escherichta coli. Appl Microbiol Biotechnol 1997;47:140-3.
10. Aoyagi A, Doi Y, Iwata T. Mechanical properties and highly ordered structure of ultra-high-molecular-weight poly[(R)-3-hydroxybutyrate] films: effects of annealing and two-step drawing. Polym Degrad Stab 2003;79:209-16.
11. Iwata T, Aoyagi Y, Fujita M, Yamane H, Doi Y, Suzuki Y, et al. Processing of a strong biodegradable poly([R]-3-hydroxybutyrate) fiber and a new fiber structure revealed by micro-beam X-ray diffraction with synchrotron radiation. Macromol Rapid Commun 2004;25:1100-4.
12. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory; 1989.
13. Amann E, Ochs B, Abel K.J. Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene 1988;69:301-15.
14. Matsusaki H, Abe H, Taguchi K, Fukui T, Doi Y. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by recombinant bacteria expressing the PHA synthase gene phaC1 from Pseudomonas sp. 61-3. Appl Microbiol Biotechnol 2000;53:401-9.
15. Taguchi K, Aoyagi Y, Matsusaki H, Fukui T, Doi Y. Coexpression of 3-ketoacyl-ACP reductase and polyhydroxyalkanoate synthase genes induces PHA production in Escherichia coli HB101 strain. FEMS Microbiol Lett 1999;176:183-90.
16. Riesenberg D. High-cell-density cultivation of Escherichia coli. Curr Opin Biotechnol 1991;2:380-4.
17. Kusaka S, Iwata T, Doi Y. Microbial synthesis and physical properties of ultra-high-molecular-weight poly[(R)-3-hydroxybutyrate]. J Macromol Sci Pure Appl Chem 1998;A35:319-35.
18. Balbas P, Bolivar F. Design and construction of expression plasmid vectors in Escherichia coli. Methods Enzymol 1990; 185: 14-37.
19. Davison J, Heusterspreute M, Chevalier N, Ha-Thi V, Brunel F. Vectors with restriction site banks. V. pJRD215, a wide-hostrange cosmid vector with multiple cloning sites. Gene 1987;51: 275-80.
20. Nagahari K, Tanaka T, Hishinuma F, Kuroda M, Sakaguchi K. Control of tryptophan synthetase amplified by varying the numbers of composite plasmids in Escherichia coli cells. Gene 1977;1:141-52.
21. Yamane T. Yield of poly-D(-)-3-hydroxybutyrate from various carbon sources: a theoretical study. Biotechnol Bioeng 1993;41: 165-70.
22. Kawaguchi Y, Doi Y. Kinetics and mechanism of synthesis and degradation of poly(3-hydroxybutyrate) in Alcaligenes eutrophus. Macromolecules 1992;25:2324-9.
23. Jossek R, Reichelt R, Steinbüchel A. In vitro biosynthesis of poly(3-hydroxybutyric acid) by using purified poly(hydroxyalkanoic acid) synthase of Chromatium vinosum. Appl Microbiol Biotechnol 1998;49:258-66.
24. Choi J, Lee SY. High level production of supra molecular weight poly(3-hydroxybutyrate) by metabolically engineered Escherichia coli. Biotechnol Bioproc Eng 2004;9:196-200.