Recovery of active medium-chain-length-poly-3-hydroxyalkanoate polymerase from inactive inclusion bodies using ion-exchange resin

Biochemical Journal

Volumn 349, Issue 2, 2000, Pages 599-604

  Ren, Qun ^a   De Roo, Guy ^a   Kessler, Birgit ^a   Witholt, Bernard ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

In vitro synthesis of polymer; Protein inclusion bodies; Pseudomonas; Recovery of enzyme activity

Indexed keywords

BACTERIAL ENZYME; DITHIOTHREITOL; GLYCEROL; ION EXCHANGE RESIN; SEPHAROSE;

ARTICLE; CELL INCLUSION; ENZYME PURIFICATION; ENZYME SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS OLEOVORANS;

ACYLTRANSFERASES; BACTERIAL PROTEINS; CHROMATOGRAPHY, ION EXCHANGE; INCLUSION BODIES; PSEUDOMONAS; RECOMBINANT PROTEINS; SOLUBILITY;

EID: 0034662156     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3490599     Document Type: Article

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