Batch production of a silk-elastin-like protein in E. coli BL21(DE3): Key parameters for optimisation

Microbial Cell Factories

Volumn 12, Issue 1, 2013, Pages

  Collins, Tony ^a   Azevedo Silva, João ^a   da Costa, André ^a   Branca, Fernando ^a   Machado, Raul ^a   Casal, Margarida ^a  

^a UNIVERSITY OF MINHO   (Portugal)

Author keywords

Batch production; Biopolymers; pET E. coli BL21(DE3); Silk elastin like polymers

Indexed keywords

ACETIC ACID; AMPICILLIN; CARBENICILLIN; ELASTIN; SILK ELASTIN LIKE PROTEIN; UNCLASSIFIED DRUG;

AERATION; ARTICLE; BIOPROCESS; DEGRADATION; ESCHERICHIA COLI; HOST CELL; NONHUMAN; PH; PLASMID; PROTEIN EXPRESSION; TEMPERATURE; VOLUMETRY;

BIOMASS; ELASTIN; ESCHERICHIA COLI; GENETIC VECTORS; HYDROGEN-ION CONCENTRATION; PLASMIDS; PROTEINS; RECOMBINANT PROTEINS; SILK; TEMPERATURE;

EID: 84874275407     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-12-21     Document Type: Article

References (49)

1. Chow D, Nunalee ML, Lim DW, Simnick AJ, Chilkoti A. Peptide-based Biopolymers in Biomedicine and Biotechnology. Mater Sci Eng R Rep 2008, 62:125-155. 10.1016/j.mser.2008.04.004, 2575411, 19122836.
2. Omenetto FG, Kaplan DL. New opportunities for an ancient material. Science 2010, 329:528-531. 10.1126/science.1188936, 3136811, 20671180.
3. Rabotyagova OS, Cebe P, Kaplan DL. Protein-based block copolymers. Biomacromolecules 2011, 12:269-289. 10.1021/bm100928x, 3071546, 21235251.
4. Urry DW, Peng SQ, Hayes LC, McPherson D, Xu J, Woods TC, Gowda DC, Pattanaik A. Engineering protein-based machines to emulate key steps of metabolism (biological energy conversion). Biotechnol Bioeng 1998, 58:175-190. 10.1002/(SICI)1097-0290(19980420)58:2/3<175::AID-BIT10>3.0.CO;2-C, 10191388.
5. Cappello J, Crissman J, Dorman M, Mikolajczak M, Textor G, Marquet M, Ferrari F. Genetic engineering of structural protein polymers. Biotechnol Prog 1990, 6:198-202. 10.1021/bp00003a006, 1366613.
6. Megeed Z, Cappello J, Ghandehari H. Genetically engineered silk-elastinlike protein polymers for controlled drug delivery. Adv Drug Deliv Rev 2002, 54:1075-1091. 10.1016/S0169-409X(02)00063-7, 12384308.
7. Guda C, Zhang X, McPherson DT, Xu J. J.H. C, Urry DW, Daniell H: Hyper expression of an environmentally friendly synthetic polymer gene. Biotechnol Lett 1995, 17:745-750.
8. Kemppainen BW, Urry DW, Swaim SF, Sartin EA, Gillette RL, Hinkle SH, Coolman SL, Luan CX, Xu J. Bioelastic membranes for topical application of a thromboxane synthetase inhibitor for protection of skin from pressure injury: a preliminary study. Wound Repair Regen 2004, 12:453-460. 10.1111/j.1067-1927.2004.12410.x, 15260811.
9. Kluge JA, Rabotyagova O, Leisk GG, Kaplan DL. Spider silks and their applications. Trends Biotechnol 2008, 26:244-251. 10.1016/j.tibtech.2008.02.006, 18367277.
10. Urry DW, Pattanaik A. Elastic protein-based materials in tissue reconstruction. Ann N Y Acad Sci 1997, 831:32-46.
11. Urry DW, Urry KD, Szaflarski W, Nowicki M. Elastic-contractile model proteins: Physical chemistry, protein function and drug design and delivery. Adv Drug Deliv Rev 2010, 62:1404-1455. 10.1016/j.addr.2010.07.001, 20655344.
12. Gustafson JA, Ghandehari H. Silk-elastinlike protein polymers for matrix-mediated cancer gene therapy. Adv Drug Deliv Rev 2010, 62:1509-1523. 10.1016/j.addr.2010.04.006, 20430059.
13. Teng W, Cappello J, Wu X. Recombinant silk-elastinlike protein polymer displays elasticity comparable to elastin. Biomacromolecules 2009, 10:3028-3036. 10.1021/bm900651g, 19788307.
14. Qiu W, Huang Y, Teng W, Cohn CM, Cappello J, Wu X. Complete recombinant silk-elastinlike protein-based tissue scaffold. Biomacromolecules 2010, 11:3219-3227. 10.1021/bm100469w, 3006068, 21058633.
15. Teng W, Cappello J, Wu X. Physical crosslinking modulates sustained drug release from recombinant silk-elastinlike protein polymer for ophthalmic applications. J Control Release 2011, 156:186-194. 10.1016/j.jconrel.2011.07.036, 3220799, 21839125.
16. Machado R, Azevedo Silva J, Correia C, Collins T, Arias J, Rodriguez-Cabello JC, Casal M. High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures. AMB Express 2013, 3:11. 10.1186/2191-0855-3-11, 3599559, 23384239.
17. Qiu W, Teng W, Cappello J, Wu X. Wet-spinning of recombinant silk-elastin-like protein polymer fibers with high tensile strength and high deformability. Biomacromolecules 2009, 10:602-608. 10.1021/bm801296r, 19186950.
18. Teule F, Cooper AR, Furin WA, Bittencourt D, Rech EL, Brooks A, Lewis RV. A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning. Nat Protoc 2009, 4:341-355. 2720753, 19229199.
19. Vendrely C, Scheibel T. Biotechnological production of spider-silk proteins enables new applications. Macromol Biosci 2007, 7:401-409. 10.1002/mabi.200600255, 17429812.
20. Collins T, Meuwis MA, Stals I, Claeyssens M, Feller G, Gerday C. A novel family 8 xylanase, functional and physicochemical characterization. J Biol Chem 2002, 277:35133-35139. 10.1074/jbc.M204517200, 12089151.
21. Babaeipour V, Abbas MP, Sahebnazar Z, Alizadeh R. Enhancement of human granulocyte-colony stimulating factor production in recombinant E. coli using batch cultivation. Bioprocess Biosyst Eng 2010, 33:591-598. 10.1007/s00449-009-0380-3, 19859744.
22. Sambrook J, Russell DW. Molecular Cloning: A laboratory manual 2001, New York: Cold Spring Harbor Laboratory Press, 3.
23. Aristidou AA, San KY, Bennett GN. Improvement of biomass yield and recombinant gene expression in Escherichia coli by using fructose as the primary carbon source. Biotechnol Prog 1999, 15:140-145. 10.1021/bp980115v, 9933525.
24. Studier FW. Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 2005, 41:207-234. 10.1016/j.pep.2005.01.016, 15915565.
25. Trabbic-Carlson K, Liu L, Kim B, Chilkoti A. Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion. Protein Sci 2004, 13:3274-3284. 2287301, 15557268.
26. Chow DC, Dreher MR, Trabbic-Carlson K, Chilkoti A. Ultra-high expression of a thermally responsive recombinant fusion protein in E. coli. Biotechnol Prog 2006, 22:638-646. 10.1021/bp0503742, 2562540, 16739944.
27. Haider M, Leung V, Ferrari F, Crissman J, Powell J, Cappello J, Ghandehari H. Molecular engineering of silk-elastinlike polymers for matrix-mediated gene delivery: biosynthesis and characterization. Mol Pharm 2005, 2:139-150. 10.1021/mp049906s, 15804188.
28. Nagarsekar A, Crissman J, Crissman M, Ferrari F, Cappello J, Ghandehari H. Genetic synthesis and characterization of pH- and temperature-sensitive silk-elastinlike protein block copolymers. J Biomed Mater Res 2002, 62:195-203. 10.1002/jbm.10272, 12209939.
29. Losen M, Frolich B, Pohl M, Buchs J. Effect of oxygen limitation and medium composition on Escherichia coli fermentation in shake-flask cultures. Biotechnol Prog 2004, 20:1062-1068. 10.1021/bp034282t, 15296430.
30. Blommel PG, Becker KJ, Duvnjak P, Fox BG. Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium composition. Biotechnol Prog 2007, 23:585-598. 2747370, 17506520.
31. Holms WH. The central metabolic pathways of Escherichia coli: relationship between flux and control at a branch point, efficiency of conversion to biomass, and excretion of acetate. Curr Top Cell Regul 1986, 28:69-105.
32. Wang H, Wang F, Wei D. Impact of oxygen supply on rtPA expression in Escherichia coli BL21 (DE3): ammonia effects. Appl Microbiol Biotechnol 2009, 82:249-259. 10.1007/s00253-008-1756-z, 19015848.
33. Tuite NL, Fraser KR, O'Byrne CP. Homocysteine toxicity in Escherichia coli is caused by a perturbation of branched-chain amino acid biosynthesis. J Bacteriol 2005, 187:4362-4371. 10.1128/JB.187.13.4362-4371.2005, 1151774, 15968045.
34. Manderson D, Dempster R, Chisti Y. A recombinant vaccine against hydatidosis: production of the antigen in Escherichia coli. J Ind Microbiol Biotechnol 2006, 33:173-182. 10.1007/s10295-005-0046-3, 16195870.
35. Shin CS, Hong MS, Bae CS, Lee J. Enhanced production of human mini-proinsulin in fed-batch cultures at high cell density of Escherichia coli BL21(DE3)[pET-3aT2M2]. Biotechnol Prog 1997, 13:249-257. 10.1021/bp970018m, 9190075.
36. Kelley KD, Olive LQ, Hadziselimovic A, Sanders CR. Look and see if it is time to induce protein expression in Escherichia coli cultures. Biochemistry 2010, 49:5405-5407. 10.1021/bi1007194, 2896908, 20540494.
37. Flickinger MC, Rouse MP. Sustaining protein synthesis in the absence of rapid cell division: an investigation of plasmid-encoded protein expression in Escherichia coli during very slow growth. Biotechnol Prog 1993, 9:555-572. 10.1021/bp00024a001, 7764344.
38. Huber R, Roth S, Rahmen N, Buchs J. Utilizing high-throughput experimentation to enhance specific productivity of an E.coli T7 expression system by phosphate limitation. BMC Biotechnol 2011, 11:22. 10.1186/1472-6750-11-22, 3068942, 21414195.
39. Rowe DC, Summers DK. The quiescent-cell expression system for protein synthesis in Escherichia coli. Appl Environ Microbiol 1999, 65:2710-2715. 91400, 10347065.
40. Korpimaki T, Kurittu J, Karp M. Surprisingly fast disappearance of beta-lactam selection pressure in cultivation as detected with novel biosensing approaches. J Microbiol Methods 2003, 53:37-42. 10.1016/S0167-7012(02)00213-0, 12609721.
41. Nair R, Salvi P, Banerjee S, Raiker VA, Bandyopadhyay S, Soorapaneni S, Kotwal P, Padmanabhan S. Yeast extract mediated autoinduction of lacUV5 promoter: an insight. N Biotechnol 2009, 26:282-288. 10.1016/j.nbt.2009.08.002, 19666150.
42. Wolfe AJ. The acetate switch. Microbiol Mol Biol Rev 2005, 69:12-50. 10.1128/MMBR.69.1.12-50.2005, 1082793, 15755952.
43. Eiteman MA, Altman E. Overcoming acetate in Escherichia coli recombinant protein fermentations. Trends Biotechnol 2006, 24:530-536. 10.1016/j.tibtech.2006.09.001, 16971006.
44. Roe AJ, O'Byrne C, McLaggan D, Booth IR. Inhibition of Escherichia coli growth by acetic acid: a problem with methionine biosynthesis and homocysteine toxicity. Microbiology 2002, 148:2215-2222.
45. Arnold CN, McElhanon J, Lee A, Leonhart R, Siegele DA. Global analysis of Escherichia coli gene expression during the acetate-induced acid tolerance response. J Bacteriol 2001, 183:2178-2186. 10.1128/JB.183.7.2178-2186.2001, 95122, 11244055.
46. Fong BA, Wood DW. Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation. Microb Cell Fact 2010, 9:77. 10.1186/1475-2859-9-77, 2978133, 20959011.
47. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685. 10.1038/227680a0, 5432063.
48. Chen PS, Toribara TY, Warner H. Microdetermination of phosphorus. AnalChem 1956, 28:1756-1758.
49. Weatherburn MW. Phenol-hypochlorite reaction for determination of ammonia. AnalChem 1967, 39:971-974.