Impact of oxygen supply on rtPA expression in Escherichia coli BL21 (DE3): Ammonia effects

Applied Microbiology and Biotechnology

Volumn 82, Issue 2, 2009, Pages 249-259

  Wang, Hengwei ^a   Wang, Fengqing ^a   Wei, Dongzhi ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Ammonia; Escherichia coli; Fed batch cultivation; Recombinant protein expression; RtPA; Scale up

Indexed keywords

AMMONIUM IONS; E. COLI; EXPRESSION LEVELS; FED-BATCH CULTIVATION; FERMENTOR; FERMENTORS; GLUTAMATE DEHYDROGENASE; NITROGEN SOURCES; NITROGEN STARVATIONS; ORGANIC NITROGEN SOURCES; OXYGEN LIMITATIONS; PROTEIN EXPRESSIONS; RECOMBINANT PROTEIN EXPRESSION; RESPIRATORY ACTIVITIES; RTPA; SCALE-UP; SHAKE FLASKS; SIGNIFICANT IMPACTS; SYNTHASE; TRYPTONE; YEAST EXTRACTS;

AMINO ACIDS; AMMONIA; AMMONIUM COMPOUNDS; BOTTLES; CELL CULTURE; CELL GROWTH; ENZYMES; ESCHERICHIA COLI; GROWTH (MATERIALS); GROWTH KINETICS; IONS; NITROGEN; NONMETALS; OXYGEN; PROTEINS;

OXYGEN SUPPLY;

ACETATE; AMMONIA; AMMONIUM; COLIFORM BACTERIUM; ENZYME ACTIVITY; EXPERIMENTAL STUDY; FERMENTATION; GENE EXPRESSION; OXYGEN; PEPTIDE; RECOMBINATION; YEAST;

AMMONIA; BIOREACTORS; CULTURE MEDIA; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; HUMANS; NITROGEN; OXYGEN; RECOMBINANT PROTEINS; TISSUE PLASMINOGEN ACTIVATOR;

ESCHERICHIA COLI;

EID: 59449109606     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1756-z     Document Type: Article

References (45)

1. MR Atkinson AJ Ninfa 1998 Role of the glnK gene in nitrogen-regulation of gene transcription in Escherichia coli Mol Microbiol 29 431 447
2. MR Atkinson TA Blauwkamp V Bondarenko V Studitsky AJ Ninfa 2002 Activation of the glnA, glnK, and nac promoters as Escherichia coli undergoes the transition from nitrogen excess growth to nitrogen starvation J Bacteriol 184 5358 5363
3. DD Axe JE Bailey 1995 Transport of lactate and acetate through the energized cytoplasmic membrane of Escherichia coli Biotechnol Bioeng 47 8 19
4. AT Bockman CA Reeve A Matin 1986 Stabilization of glucose-starved Escherichia coli K-12 and Salmonella typhimurium LT2 by peptidase-deficient mutants J Gen Microbiol 132 231 235
5. FC Boogerd P Bos JG Kuenen JJ Heijnen RG van der Lans 1990 Oxygen and carbon dioxide mass transfer and the aerobic, autotrophic cultivation of moderate and extreme thermophiles: A case study related to the microbial desulfurization of coal Biotechnol Bioeng 35 1111 1119
6. BR Ernsting JW Denninger RM Blumenthal RG Matthews 1993 Regulation of the gltBDF operon of Escherichia coli: how is a leucine-insensitive operon regulated by the leucine-responsive regulatory protein? J Bacteriol 175 7160 7169
7. P Fossati L Prencipe 1982 Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide Clin Chem 28 2077 2080
8. R Grabherr E Nilsson G Striedner K Bayer 2002 Stabilizing plasmid copy number to improve recombinant protein production Biotechnol Bioeng 77 142 147
9. RG Groat JE Schultz E Zychlinsky A Bockman A Matin 1986 Starvation proteins in Escherichia coli: kinetics of synthesis and role in starvation survival J Bacteriol 168 486 493
10. JA Imlay 2003 Pathways of oxidative damage Annu Rev Microbiol 57 395 418
11. A Javelle E Severi J Thornton M Merrick 2004 Ammonium sensing in Escherichia coli: Role of the ammonium transporter AmtB and AmtB-GlnK complex formation J Biol Chem 279 8530 8538
12. RP Jeanette LO Dale 1968 Amino acid transport systems in Escherichia coli K12 J Biol Chem 243 5914 5920
13. EB Jensen S Carlsen 1990 Production of recombinant human growth hormone in Escherichia coli: expression of different precursors and physiological effects of glucose, acetate, and salts Biotechnol Bioeng 36 1 11
14. D Kleiner 1985 Bacterial ammonium transport FEMS Microbiol Rev 32 87 100
15. GL Kleman WR Strohl 1994 Acetate metabolism by Escherichia coli in high-cell-density fermentation Appl Environ Microbiol 60 3952 3958
16. UK Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680 685
17. DR Lasko N Zamboni U Sauer 2000 Bacterial response to acetate challenge: a comparion of tolerance among species Appl Microbiol Biotechnol 54 243 247
18. SY Lee 1996 High cell-density culture of Escherichia coli Trends Biotechol 14 98 105
19. JA Leigh JA Dodsworth 2007 Nitrogen regulation in bacteria and archaea Annu Rev Microbiol 61 349 77
20. M Losen B Frölich M Pohl J Büchs 2004 Effect of oxygen limitation and medium composition on Escherichia coli fermentation in shake-flask cultures Biotechnol Prog 20 1062 1068
21. OH Lowry NJ Rosebrough AL Farr RJ Randall 1951 Protein measurement with the Folin phenol reagent J Biol Chem 193 265 275
22. C Lu WE Bentley G Rao 2003 Comparisons of oxidative stress response genes in aerobic Escherichia coli fermentations Biotechnol Bioeng 83 864 870
23. SC Makrides 1996 Strategies for achieving high-level expression of genes in Escherichia coli Microbiol Rev 60 512 538
24. W Margareta H Erik A Anneli K Christina L Bjorn R Benny H Lennart P Gunnar 1991 Synthesis and secretion of a fibrinolytically active tissue-type plasminogen activator variant in Escherichia coli Gene 99 243 248
25. MR Maurizi F Rasulova 2002 Degradation of L-glutamate dehydrogenase from Escherichia coli: allosteric regulation of enzyme stability Arch Biochem Biophys 397 206 216
26. E McFall EB Newman 1996
27. MJ Merrick RA Edwards 1995 Nitrogen control in bacteria Microbiol Rev 59 604 622
28. RE Miller ER Stadtman 1972 Glutamate synthase from Escherichia coli. An iron-sulfide flavoprotein J Biol Chem 247 7407 7419
29. F Naider JM Becker 1975 Multiplicity of oligopeptide transport systems in Escherichia coli J Bacteriol 122 1208 1215
30. P Neubauer HY Lin B Mathiszik 2003 Metabolic load of recombinant protein production: Inhibition of cellular capacities for glucose uptake and respiration after induction of a heterologous gene in Escherichia coli Biotechnol Bioeng 83 53 64
31. AJ Ninfa MR Atkinson 2000 PII signal transduction proteins Trends Microbiol 8 172 179
32. JW Payne G Bell 1979 Direct determination of the properties of peptide transport systems in Escherichia coli, using a fluorescent-labeling procedure J Bacteriol 137 447 455
33. MW Qoronfleh 1999 Dissolved oxygen concentration affects the accumulation of HIV-1 recombinant proteins in Escherichia coli Appl Biochem Biotechnol 80 107 120
34. L Reitzer 2003 Nitrogen assimilation and global regulation in Escherichia coli Annu Rev Microbiol 57 155 176
35. 54-dependent genes in Escherichia coli Microbiol Mol Biol Rev 65 422 444
36. W Ryan SJ Parulekar BC Stark 1989 Expression of β-lactamase by recombinant Escherichia coli strains containing plasmids of different sizes - effects of pH, phosphate, and dissolved oxygen Biotechnol Bioeng 34 309 319
37. Y Saito Y Ishii H Sasaki M Hayashi T Fujimura Y Imai S Nakamura S Suzuki J Notani T Asada H Horiai K Masakazu N Mineo 1994 Production and characterization of a novel tissue-type plasminogen activator derivative in Escherichia coli Biotechnol Prog 10 472 479
38. PJ Senior 1975 Regulation of nitrogen metabolism in Escherichia coli and Klebsiella aerogenes: studies with the continuous-culture technique J Bacteriol 123 407 418
39. L a) using the dynamic "gas out-gas in" method: analysis of errors caused by dissoved oxygen probes Biotechnol Bioeng 46 388 392
40. JH Verheijen E Mullaart GT Chang C Kluft G Wijngaards 1982 A simple, sensitive spectrophotometric assay for extrinsic (tissue-type) plasminogen activator applicable to measurements in plasma Thromb Haemost 48 266 269
41. MW Weatherburn 1967 Phenol-hypochlorite reaction for determination of ammonia Anal Chem. 39 971 974
42. CA Woolfolk B Shapiro ER Stadtman 1966 Regulation of glutamine synthetase. I. Purification and properties of glutamine synthetase from Escherichia coli Arch Biochem Biophys 116 177 192
43. YC Zhao G Wang Y Kong CK Zhang 2003 Cloning, expression and renaturation studies of retaplase J Microbiol Biotechnol 13 989 992
44. L Zheng D Kostrewa S Bernèche FK Winkler XD Li 2004 The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli Proc Natl Acad Sci U S A 101 17090 17095
45. DP Zimmer E Soupene HL Lee VF Wendisch AB Khodursky BJ Peter RA Bender S Kustu 2000 Nitrogen regulatory protein C-controlled genes of Escherichia coli: scavenging as a defense against nitrogen limitation Proc Natl Acad Sci U S A 97 14674 14679