Modular exchange of substrate-binding loops alters both substrate and cofactor specificity in a member of the aldo-keto reductase superfamily

Protein Engineering, Design and Selection

Volumn 26, Issue 3, 2013, Pages 181-186

  Campbell, Elliot ^a   Chuang, Sara ^a   Banta, Scott ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

aldo keto reductase; enzyme catalysis; oxidoreductases; protein stability; substrate specificity

Indexed keywords

ALDO-KETO REDUCTASE; ENZYME CATALYSIS; OXIDO-REDUCTASES; PROTEIN STABILITY; SUBSTRATE SPECIFICITY;

ENZYME ACTIVITY; GRAFTING (CHEMICAL);

SUBSTRATES;

ALCOHOL DEHYDROGENASE; ALCOHOL DEHYDROGENASE D; ALDEHYDE REDUCTASE; ALDO KETO REDUCTASE; GLYCERALDEHYDE; OXIDOREDUCTASE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CHIMERA; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MODIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; HUMAN; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PYROCOCCUS FURIOSUS; SPECIES DIFFERENCE; TURNOVER TIME;

ALCOHOL DEHYDROGENASE; ALDEHYDE REDUCTASE; AMINO ACID SEQUENCE; GLYCERALDEHYDE; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN STABILITY; PYROCOCCUS FURIOSUS; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84874223993     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs095     Document Type: Article

References (27)

1. Askonas,L.J., Ricigliano,J.W. and Penning,T.M. (1991) Biochem. J, 278, 835-841
2. Banta,S., Swanson,B.A., Wu,S., Jarnagin,A. and Anderson,S. (2002) Protein Eng., 15, 131-140. DOI 10.1093/protein/15.2.131
3. Bohren,K., Page,J., Shankar,R., Henry,S. and Gabbay,K. (1991) J. Biol. Chem., 266, 24031-24037
4. Campbell,E., Wheeldon,I.R. and Banta,S. (2010) Biotechnol. Bioeng., 107, 763-774. DOI 10.1002/bit.22869
5. Cooper,W.C., Jin,Y. and Penning,T.M. (2007) J. Biol. Chem., 282, 33484-33493. DOI 10.1074/jbc.M703414200
6. Crosas,B., Hyndman,D.J., Gallego,O., Martras,S., Pares,X., Flynn,T.G. and Farres,J. (2003) Biochem. J., 373, 973-979. DOI 10.1042/bj20021818
7. Di Luccio,E., Elling,R.A. and Wilson,D.K. (2006) Biochem. J., 400, 105-114. DOI 10.1042/bj20060660
8. Ellis,E.M. (2002) FEMS Microbiol. Lett., 216, 123-131. DOI 10.1111/ j.1574-6968.2002.tb11425.x.
9. Fersht,A. (1985) Enzyme structure and mechanism, 2nd edn. W.H. Freeman, New York
10. Grimshaw,C.E., Bohren,K.M., Lai,C.J. and Gabbay,K.H. (1995 Biochemistry, 34, 14366-14373
11. Jackman,M., Parry,M., Hofsteenge,J. and Stone,S. (1992) J. Biol. Chem., 267, 15375-15383
12. Jez,J.M. and Penning,T.M. (2001) Chem. Biol. Interact., 130-132, 499-525
13. Jez,J.M., Bennett,M.J., Schlegel,B.P., Lewis,M. and Penning,T.M. (1997 Biochem. J., 326, 625-636
14. Kubiseski,T.J. and Flynn,T.G. (1995) J. Biol. Chem., 270, 16911-16917. DOI 10.1074/jbc.270.28.16911
15. Kubiseski,T.J., Hyndman,D.J., Morjana,N.A. and Flynn,T.G. (1992) J. Biol. Chem., 267, 6510-6517
16. Leitgeb,S., Petschacher,B., Wilson,D.K. and Nidetzky,B. (2005) FEBS Lett., 579, 763-767
17. Ma,H. and Penning,T.M. (1999) Proc. Natl Acad. Sci. USA, 96, 11161-11166
18. Machielsen,R., Uria,A.R., Kengen,S.W.M. and van der Oost,J. (2006) Appl. Environ. Microbiol., 72, 233-238. DOI 10.1128/aem.72.1.233-238.2006
19. Penning,T.M. (1999) J. Steroid Biochem. Mol. Biol., 69, 211-225
20. Ratnam,K., Ma,H. and Penning,T.M. (1999) Biochemistry, 38, 7856-7864
21. Sanli,G. and Blaber,M. (2001) J. Mol. Biol., 309, 1209-1218
22. Sanli,G., Dudley,J.I. and Blaber,M. (2003) Cell Biochem. Biophys., 38, 79-101
23. Scoble,J., McAlister,A.D., Fulton,Z., et al. (2010) J. Mol. Biol., 398, 26-39. DOI DOI: 10.1016/j.jmb.2010.02.021
24. Segel,I.H. (1993) Enzyme kinetics : Behavior and analysis of rapid equilibrium and steady state enzyme systems. Wiley Classics Library edn, Wiley, New York
25. Stone,S.R. and Le Bonniec,B.F. (1997) J. Mol. Biol., 265, 344-362
26. Tarle,I., Borhani,D.W., Wilson,D.K., Quiocho,F.A. and Petrash,J.M. (1993 J. Biol. Chem., 268, 25687-25693
27. Wheeldon,I.R., Campbell,E. and Banta,S. (2009) J. Mol. Biol., 392, 129-142