Development and validation of a modified broad-range 16S rDNA PCR for diagnostic purposes in clinical microbiology

Journal of Microbiological Methods

Volumn 79, Issue 2, 2009, Pages 227-231

  Cherkaoui, Abdessalam ^a   Emonet, Stéphane ^a   Ceroni, Dimitri ^a   Candolfi, Bruno ^a   Hibbs, Jonathan ^a   Francois, Patrice ^a   Schrenzel, Jacques ^a  

^a GENEVA UNIVERSITY HOSPITALS   (Switzerland)

Author keywords

Broad range PCR; Contaminating DNA; Exonuclease III; Molecular methods; Taq DNA polymerase

Indexed keywords

ANTIBIOTIC AGENT; BACTERIAL DNA; DNA 16S; EXODEOXYRIBONUCLEASE III;

ADOLESCENT; ADULT; AGED; ARTICLE; BACTERIAL INFECTION; BACTERIUM IDENTIFICATION; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC TEST; DNA SEQUENCE; FALSE POSITIVE RESULT; FEMALE; GENE AMPLIFICATION; HUMAN; HUMAN TISSUE; INFECTION RISK; MALE; MICROBIOLOGY; NONHUMAN; PATHOGENESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VALIDATION PROCESS;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BACTERIA; BACTERIAL INFECTIONS; BACTERIOLOGICAL TECHNIQUES; CHILD; CHILD, PRESCHOOL; DNA PRIMERS; DNA, BACTERIAL; DNA, RIBOSOMAL; FEMALE; HUMANS; INFANT; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; YOUNG ADULT;

BACTERIA (MICROORGANISMS);

EID: 70349958003     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2009.09.014     Document Type: Article

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