Effects of phytoestrogen a-zal and mechanical stimulation on proliferation, osteoblastic differentiation, and opg/rankl expression in MC3T3-E1 pre-osteoblasts

Cellular and Molecular Bioengineering

Volumn 5, Issue 4, 2012, Pages 427-439

  Liu, Lu ^a,b   Guo, Yong ^a   Wan, Zongming ^a   Shi, Caihong ^a   Li, Jianyu ^a   Li, Ruixin ^a,b   Hao, Qingxin ^a   Li, Hao ^a   Zhang, Xizheng ^a  

^a BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

^b MEDICAL COLLEGE OF CHINESE PEOPLE'S ARMED POLICE FORCES   (China)

Author keywords

Bone formation; Mechanical strain; Osteoporosis; Zearalanol

Indexed keywords

ALKALINE PHOSPHATASE; ALPHA ZERANOL; MESSENGER RNA; OSTEOCLAST DIFFERENTIATION FACTOR; OSTEOPROTEGERIN; TRANSCRIPTION FACTOR RUNX2; UNCLASSIFIED DRUG; ZERANOL;

ALKALINE PHOSPHATASE GENE; ANIMAL CELL; ARTICLE; BONE DEVELOPMENT; CELL ASSAY; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL STIMULATION; CONTROLLED STUDY; DNA SEQUENCE; DRUG EFFECT; DRUG MECHANISM; ENZYME ACTIVITY; FLOW CYTOMETRY; GENE; GENE EXPRESSION; GENETIC MARKER; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; OSTEOBLAST; OSTEOCLAST; OSTEOPROTEGERIN GENE; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RUNT RELATED TRANSCRIPTIONAL FACTOR 2 GENE; SPECTROPHOTOMETRY; UPREGULATION; WESTERN BLOTTING;

EID: 84874107432     PISSN: 18655025     EISSN: 18655033     Source Type: Journal    
DOI: 10.1007/s12195-012-0244-9     Document Type: Article

References (48)

1. Astrid, L., W. Liane, S. Lothar, et al. Estrogen receptor and Wnt signaling interact to regulate early gene expression in response to mechanical strain in osteoblastic cells. Biochem. Biophys. Res. Commun. 394:755-759, 2010.
2. Boyle, W. J., W. S. Simonet, and D. L. Lacey. Osteoclast differentiation and activation. Nature 423:337-342, 2003.
3. Chi, H. K., Y. Lidan, and E. Clare. Oscillatory fluid flowinduced shear stress decreases osteoclastogenesis. Bone 39:1043-1047, 2006.
4. Cotter, A., and K. D. Cashman. Genistein appears to prevent early postmenopausal bone loss as effectively as hormone replacement therapy. Nutr. Rev. 61(10):346-351, 2003.
5. Dai, S. L., J. Duan, Y. Lu, et al. A new phytoestrogen a-Zearalanol markedly inhibits progression of atherogenesis in ovariectomized cholesterol-fed rabbits. Mol. Cell Cardiol. 34:24-27, 2002.
6. Donna, C. Prevention of osteoporosis: from infancy through older adulthood. Hong Kong Physiother. J. 30: 6-12, 2012.
7. Drissi, H., Q. Luc, I. R. Shakoor, et al. Transcriptional autoregulation of the bone related CBFa1/RUNX2 gene. Cell Physiol. 184:341-350, 2000.
8. Ducy, P., R. Zhang, V. Geoffroy, et al. Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell 89:747-754, 1997.
9. Elisabetta, L., P. Letizia, T. Elisa, et al. Human estrogen receptor a gene is a target of Runx2 transcription factor in osteoblasts. Exp. Cell Res. 313:1548-1560, 2007.
10. Fujita, M., T. Urano, K. Horie, et al. Estrogen activates cyclin-dependent kinases 4 and 6 through induction of cyclin D in rat primary osteoblasts. Biochem. Biophys. Res. Commun. 299:222-228, 2002.
11. Gohel, A., M. B. McCarthy, and G. Gronowicz. Estrogen prevents gulcocorticoid-induced apoptosis in osteoblasts in vivo and in vitro. Endocrinology 140:5339-5347, 1999.
12. Iacovino, J. R. Mortality outcomes after osteoporotic fractures in men and women. Insur. Med. 33:316-320, 2001.
13. Kaneuji, T., S. Nogami, W. Ariyoshi, et al. Regulatory effect on osteoclastogenesis of mechanical strain-loaded osteoblasts. Int. J. Oral Maxillofac. Surg. 40(10):1215, 2011.
14. Lacey, D. L., E. Timms, H. L. Tan, et al. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell 93:165-176, 1998.
15. Lanyon, L., V. Armstrong, D. Ong, et al. Is estrogen receptor alpha key to controlling bones' resistance to fracture Endocrinology 182:183-191, 2004.
16. Lee, K. C., H. Jessop, R. Suswillo, et al. The adaptive response of bone to mechanical loading in female transgenic mice is deficient in the absence of estrogen receptoralpha and-beta. Endocrinology 182:193-201, 2004.
17. Lee, M. H., Y. J. Kim, H. J. Kim, et al. BMP-2-induced Runx2 expression is mediated by Dlx5, and TGF-b1 opposes the BMP-2-induced osteoblast differentiation by suppression of Dlx5 expression. Biol. Chem. 278:34387-34394, 2003.
18. Livak, K. J., and T. D. Schmittgen. Analysis of relative gene expression data using realtime quantitative PCR and the 2-DDCT method. Methods 25:402-408, 2001.
19. Lorraine, A. F. Estrogen therapy for postmenopausal osteoporosis. Arq. Bras. Endocrinol. Metab. 50(4):705-719, 2006.
20. Ludwika, K., L. Astrid, H. Sofia, et al. Mechanical regulation of osteoclastic genes in human osteoblasts. Biochem. Biophys. Res. Commun. 368:582-587, 2008.
21. Marcus, R. Antiresorptive treatment of postmenopausal osteoporosis: comparison of study designs and outcomes in large clinical trials with fracture as an endpoint. Endocr. Rev. 23:16-37, 2002.
22. Martin, R. B. Toward a unifying theory of bone remodeling. Bone 26:1-6, 2000.
23. Meilin, W., H. Eric, M. Frederic, et al. Zfp521 antagonizes Runx2, delays osteoblast differentiation in vitro, and promotes bone formation in vivo. Bone 44:528-536, 2009.
24. Okazaki, R., D. Inoue, M. Shibata, et al. Estrogen promotes early osteoblast differentiation and inhibits adipocyte differentiation in mouse bone marrow stromal cell lines the express estrogen receptor(ER) alpha or beta. Endocrinology 143:2349-2356, 2002.
25. Papachroni, K. K., D. N. Karatzas, K. A. Papavassiliou, et al. Mechanotransduction in osteoblast regulation and bone disease. Trends Mol. Med. 15:208-216, 2009.
26. Pérez-Casellas, L. A., X. Wang, et al. Nuclear Factor I transcription factors regulate IGF binding protein 5 gene transcription in human osteoblasts. Biochim. Biophys. Acta 1789(2):78-87, 2009.
27. Reid, I. R. Anti-resorptive therapies for osteoporosis. Semin. Cell Dev. Biol. 19:473-478, 2008.
28. Resca, E., G. Carnevale, A. Benelli, et al. Influence of ferutinin on bone metabolism in ovariectomized rats. II: role in recovering osteoporosis. J. Anat. 217(1):48-56, 2010.
29. Ruimerman, R., P. Hilbers, B. van Rietbergen, and R. Huiskes. A theoretical framework for strain-related trabecular bone maintenance and adaptation. Biomechanics 38:931-941, 2005.
30. Rumney, R. M. H., A. Sunters, G. C. Reilly, et al. Application of multiple forms of mechanical loading to human osteoblasts reveals increased ATP release in response to fluid flow in 3D cultures and differential regulation of immediate early genes. J. Biomech. 45(3):549-554, 2012.
31. Sanaa, E., K. Gamal, A. M. Fayda, et al. Real-time PCR hTERT mRNA pattern in tumor core, edge, resection margin, and lymph nodes in laryngeal tumors: relation to proliferative index and impact on prognosis. Clin. Biochem. 38:873-878, 2005.
32. Sanchez, C., O. Gabay, C. Salvat, et al. Mechanical loading highly increases IL-6 production and decreases OPG expression by osteoblasts. Osteoarthr. Cartil. 17:473-481, 2009.
33. Schoppet, M., K. T. Preissner, L. C. Hofbauer, et al. RANK ligand and osteoprotegerin: paracrine regulators of bone metabolism and vascular function. Arterioscler. Thromb. Vasc. Biol. 22:549-553, 2002.
34. Seelig, M. S., B. M. Altura, and B. T. Altura. Benefits and risks of sex hormone replacement in postmenopausal women. Am. Coll. Nutr. 23:482S-496S, 2004.
35. Shen, R., M. Chen, Y. J. Wang, et al. Smad6 interacts with Runx2 and mediates Smad ubiquitin regulatory factor 1-induced Runx2 degradation. Biol. Chem. 281:3569-3576, 2006.
36. Shireen, K., P. H. V. Calvin, G. Yuguang, et al. Collagen, type V, a1 (COL5A1) is regulated by TGF-b in osteoblasts. Matrix Biol. 23(7):445-455, 2004.
37. Syed, F., and S. Khosla. Mechanisms of sex steroid effects on bone. Biochem. Biophys. Res. Commun. 328:688-696, 2005.
38. Tang, D. Z., W. Hou, Q. Zhou, et al. Osthole stimulates osteoblast differentiation and bone formation by activation of beta-catenin-BMP signaling. J. Bone Miner. Res. 25:1234-1245, 2010.
39. Tang, L. L., Y. L. Wang, J. Pan, and S. X. Cai. The effect of step-wise increased stretching on rat calvarial osteoblast collagen production. Biomechanics 37:157-161, 2004.
40. Tang, L., H. Zhao, P. Shao, et al. Preliminary investigation of signal transduction pathway on expression levels of OPG/RANKL that mediated cellular responses to mechanical strain in MC3T3-E1 cells. Clin. Stomatol. 26:202-204, 2010.
41. Tatsuyama, K., Y. Maezawa, H. Baba, et al. Expression of various growth factors for cell proliferation and cytodifferentiation during fracture repair of bone. Eur. J. Histochem. 44(3):269-278, 2000.
42. Theoleyre, S., Y. Wittrant, S. K. Tat, et al. The molecular triad OPG/RANK/RANKL: involvement in the orchestration of pathophysiological bone remodeling. Cytokine Growth Factor Rev. 15:457-475, 2004.
43. Wang, L., X. Zhang, Y. Guo, et al. Involvement of BMPs/Smad signaling pathway in mechanical response in osteoblasts. Cell. Physiol. Biochem. 26:1093-1102, 2010.
44. Xiao, Z. S., S. G. Liu, T. K. Hinson, et al. Characterization of the upstream mouse Cbfa1/Runx2 Promoter. Cell Biochem. 82:647-659, 2001.
45. Yan, Y., Y. Gong, Y. Guo, et al. Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation. PLoS ONE 7(4):35709, 2012.
46. Yan, Y., M. Song, C. Guo, et al. The effects of substratestretching strain on the BMP-2 mRNA expression in three kinds of mouse cell lines. Chin. J. Gerontol. 30:3092-3095, 2010.
47. Young, D. W., M. Q. Hassan, X. Q. Yang, et al. Mitotic retention of gene expression patterns by the cell fatedetermining transcription factor Runx2. Proc. Nat. Acad. Sci. USA 104:3189-3194, 2007.
48. Zaman, G., M. Z. Cheng, and H. L. Jessop. Mechanical strain activates estrogen response elements in bone cells. Bone 27:233-239, 2000.