Application of multiple forms of mechanical loading to human osteoblasts reveals increased ATP release in response to fluid flow in 3D cultures and differential regulation of immediate early genes

Journal of Biomechanics

Volumn 45, Issue 3, 2012, Pages 549-554

  Rumney, R M H ^a   Sunters, A ^b   Reilly, G C ^c   Gartland, A ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b ROYAL VETERINARY COLLEGE   (United Kingdom)

^c UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

ATP; Immediate early gene; Mechanical stimuli; Mechanostat; Osteoblast

Indexed keywords

3D CULTURE; BONE REMODELLING; CELL TYPES; COMPRESSIVE LOADING; DIFFERENTIAL REGULATION; EXTRACELLULAR ENVIRONMENTS; GENE ACTIVATION; HUMAN OSTEOBLAST; LAMINAR FLUID FLOW; MECHANICAL LOADING; MECHANICAL STIMULUS; MECHANOSTAT; MONOLAYER CULTURE; OSTEOBLAST CELLS; OSTEOBLAST CULTURES; REAL-TIME PCR; REPORTER CELL LINE; SUBSTRATE STRAIN; TURBULENT FLUID FLOW;

ADENOSINETRIPHOSPHATE; BONE; CELL CULTURE; FLUIDS; GENES; LOADING; MONOLAYERS; OSTEOBLASTS; THREE DIMENSIONAL;

FLOW OF FLUIDS;

ADENOSINE TRIPHOSPHATE; CYCLOOXYGENASE 2; MESSENGER RNA; PROTEIN C FOS;

ARTICLE; CELL ACTIVATION; CELL TYPE; CONTROLLED STUDY; FLOW RATE; FLUID FLOW; GENE ACTIVATION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMEDIATE EARLY GENE; LOADING TEST; MECHANICAL STIMULATION; MOLECULAR DYNAMICS; OSTEOBLAST; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; THREE DIMENSIONAL IMAGING; TRANSCRIPTION REGULATION; TURBULENT FLOW;

ADENOSINE TRIPHOSPHATE; CELL LINE; GENE EXPRESSION REGULATION; GENES, FOS; GENES, IMMEDIATE-EARLY; HUMANS; OSTEOBLASTS; RNA, MESSENGER; SIGNAL TRANSDUCTION; STRESS, MECHANICAL;

EID: 84856054091     PISSN: 00219290     EISSN: 18732380     Source Type: Journal    
DOI: 10.1016/j.jbiomech.2011.11.036     Document Type: Article

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